Cationic liposome nanoparticles co-loaded with HBx-siRNA and schisandra chinensis oil as well as preparation method and application thereof

A cationic liposome and schisandra oil technology, which is applied in liposome delivery, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., can solve the problem that it is difficult to completely eliminate the hidden dangers of cancer caused by viruses

Active Publication Date: 2021-09-03
HEILONGJIANG UNIV OF CHINESE MEDICINE
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Problems solved by technology

Hepatitis B virus can replicate itself and carry out the reverse transcription process, so the viral genome can be integrated into the body, making it difficult for the virus to be completely eliminated in the body and there is a potential carcinogenic hidden danger

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  • Cationic liposome nanoparticles co-loaded with HBx-siRNA and schisandra chinensis oil as well as preparation method and application thereof
  • Cationic liposome nanoparticles co-loaded with HBx-siRNA and schisandra chinensis oil as well as preparation method and application thereof
  • Cationic liposome nanoparticles co-loaded with HBx-siRNA and schisandra chinensis oil as well as preparation method and application thereof

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Embodiment Construction

[0026] The following clearly and completely describes the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, but not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention. The used materials, reagents, etc., unless otherwise specified, are commercially available reagents and materials.

[0027] Experimental Materials

[0028] (1) Schisandra oil batch number: 2018-10-22-1 Jiangxi Senhai Vegetable Oil Co., Ltd.;

[0029] (2) HBx-siRNA was synthesized by Suzhou Gemma Gene Co., Ltd. The HBx-siRNA sequence is: sense strand 5'-GAA UCC UCA CAA UAC CGC ATT-3', antisense strand 5'-UGA GAG UCC AAG AGU CCU CTT-3'.

[0030] Optimization of preparation process conditions of Schisandra oil cationic liposomes ...

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Abstract

The invention relates to the technical field of nano-drugs, in particular to cationic liposome nanoparticles co-loaded with HBx-siRNA and schisandra chinensis oil as well as a preparation method and application thereof. According to the invention, small interfering RNA (Ribonucleic Acid) aiming at HBx is established, schisandra chinensis oil with a liver protection effect is selected, and water-soluble substances and fat-soluble substances are simultaneously loaded in the cationic liposome by utilizing the characteristic that the cationic liposome can simultaneously load the water-soluble substances and the fat-soluble substances, so that the preparation process is optimized; and the cationic liposome nanoparticles co-loaded with the HBx-siRNA and the schisandra chinensis oil, which are prepared by the optimal process, are subjected to pharmacodynamic research on in-vitro inhibition of hepatitis B. Experiments prove that the schisandra chinensis oil in the cationic liposome nanoparticles has a good effect of cooperating with HBx-siRNA to inhibit hepatitis B.

Description

technical field [0001] The application relates to the technical field of nanomedicine, in particular to a cationic liposome nanoparticle co-carrying HBx-siRNA and Schisandra oil, a preparation method and its application. Background technique [0002] Liposomes are composed of natural or synthetic phospholipids and cholesterol and have good biocompatibility. The main function of cholesterol is to adjust the fluidity of the lipid bilayer, making the carrier more stable. Two layers of phospholipid molecules are arranged to form a sphere that can simultaneously wrap water-soluble components and fat-soluble components. The liposome-mediated preparation is simple, the raw materials have no toxic and side effects, and can prevent the target gene from being degraded by ribozymes. The hydrophilic and hydrophobic groups of cationic liposomes are important components of cationic liposomes. DNA / RNA containing negative charges can be transported through the cell membrane with positivel...

Claims

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Application Information

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IPC IPC(8): A61K9/51A61K47/10A61K36/57A61K47/69A61K31/713A61K47/61A61K47/64A61K48/00A61P1/16A61P31/20
CPCA61K31/713A61K47/61A61K47/64A61K9/5146A61K36/57A61K47/6911A61K48/005A61K48/0008A61P1/16A61P31/20A61K2300/00
Inventor 杨婧王锐李娅兰白皓天
Owner HEILONGJIANG UNIV OF CHINESE MEDICINE
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