Application of meclofenoxate hydrochloride in preparation of medicine for preventing or treating Parkinson's disease

A technology for meclofen axetil hydrochloride and Parkinson's disease, which can be used in drug combinations, pharmaceutical formulations, nervous system diseases, etc., can solve problems such as incurable and incapable of preventing the development of diseases, reduce mitochondrial membrane damage, improve behavioral symptoms, Produce reliable results

Pending Publication Date: 2021-09-03
SUN YAT SEN MEMORIAL HOSPITAL SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, whether the disease is treated with drugs or surgery, it can only imp...

Method used

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  • Application of meclofenoxate hydrochloride in preparation of medicine for preventing or treating Parkinson's disease
  • Application of meclofenoxate hydrochloride in preparation of medicine for preventing or treating Parkinson's disease
  • Application of meclofenoxate hydrochloride in preparation of medicine for preventing or treating Parkinson's disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Determination of Primary Cell Synapse Morphology

[0021] Purchasing 0-day C57 suckling mice to extract cortical neuron cells, after the neuron cells grow synapses, give MH (1μM) to treat for 2h, then add ROT (400μM) to construct PD cell model, observe the morphology of cell synapses after 24h, apply tubulin Neural synaptic tubulin was visualized, neuron cells were visualized by Neu N, and BX86 was used to observe.

[0022] The result is as figure 1 As shown, there was no significant difference between MH and NC neuronal cells. A large number of neuron cells in the ROT-constructed PD group died, and the number of surviving cells in each visual field was 119.33 less than that in the NC group, p=0.037. The survival of neuron cells in the ROT+MH group was lower than that in the NC group and higher than that in the ROT group. The number of surviving cells in each field of view was 40.33 more than that in the ROT group, p=0.028, which was statistically different....

Embodiment 2

[0023] Embodiment 2 animal experiments and results

[0024] 2.1 Experimental materials and equipment

[0025] 2.1.1 Experimental objects

[0026] The Parkinson's (PD) mouse model, C57BL / 6J mice, was provided by Beijing Weitong Lihua Experimental Animal Technology Co., Ltd. (animal license number: SCXK (Beijing) 2016-0011).

[0027] 2.1.2 Experimental conditions

[0028] Clean grade animal room with a temperature of 22-25°C, a relative humidity of 40% to 60%, a 12 / 12h light and dark cycle, and free access to food and water. During the experiment, the animals were raised and collected in accordance with the relevant regulations on the management and protection of experimental animals.

[0029] 2.1.3 Main experimental equipment

[0030] Roche Modular-ISE9OO-P800 automatic biochemical analyzer; electronic balance BP12lS; LDZ5-2 desktop low-speed automatic balance centrifuge; Spectrumlab22PC spectrophotometer; Nippon Kohden MEK-6318K automatic blood cell analyzer; LEICARM2135 ...

Embodiment 3

[0049] Embodiment 3 statistical analysis

[0050] All GTVPET-AS profiles were compared to GTVPET using DSC calculated by Matlab 2016b (MathWorks Inc., Natick, MA, USA). The mean and standard deviation (SD) of each indicator were also calculated. Results were analyzed using the Kruskal-Wallis test. All values ​​in figures and text are expressed as mean ± standard error of the mean (SEM). Motor performance in behavioral tests is expressed in absolute values. Differences between groups were analyzed by an analysis of variance (ANOVA) using Dunnett and Tukey posttests for comparison. p<0.05 was considered statistically significant (n=number of mice included in each analysis). Statistical analysis was performed using SPSS 19.

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Abstract

The invention relates to the field of biological pharmacy, in particular to application of meclofenoxate hydrochloride (MH) in preparation of a medicine for preventing or treating Parkinson's disease. By integrating multi-omics data to explore a gene co-expression network and applying cell and animal experiments to verify, the meclofenoxate hydrochloride can obviously prevent synaptic injury caused by mitochondrial function decline in the Parkinson's disease, can effectively reduce mitochondrial membrane injury and reduce mitochondrial edema, and can effectively enable the expression of synapsis specific proteins to rise; it is indicated that the Parkinson's disease characterized by neuronal synaptic lesion can be prevented, dopamine activity is improved, Parkinson's disease lesion is prevented, and behavioral symptoms of the Parkinson's disease is improved.

Description

technical field [0001] The invention relates to the field of biopharmaceuticals, in particular to the application of meclofenoxate hydrochloride in the preparation of medicaments for preventing or treating Parkinson's disease. Background technique [0002] Parkinson's disease (PD), the second most common neurodegenerative disease in the world, is a central nervous system degenerative disease characterized by degeneration and necrosis of dopamine neurons in the substantia nigra of the midbrain. At present, no matter drug treatment or surgical treatment of the disease can only improve its symptoms, it cannot prevent the development of the disease, let alone cure it. [0003] Meclofenoxate hydrochloride (MH) is a central stimulant drug, generally used in patients with traumatic brain injury coma, neonatal hypoxia and mental confusion. Contents of the invention [0004] The purpose of the present invention is to overcome the deficiencies of the prior art, and provide a kind o...

Claims

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Application Information

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IPC IPC(8): A61K31/216A61P25/16
CPCA61K31/216A61P25/16
Inventor 赵慧英张华宋范聪刘菲怡
Owner SUN YAT SEN MEMORIAL HOSPITAL SUN YAT SEN UNIV
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