Glutathione-rich yeast extract and preparation method thereof as well as application

A yeast extract and glutathione technology, applied in the field of yeast extract, can solve the problem of poor whitening and antioxidant effect, low free radical scavenging ability and tyrosinase activity inhibition strength, and low glutathione content And other issues

Pending Publication Date: 2021-09-07
ANGELYEAST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The technical problem to be solved by the present invention: the yeast extract produced by the prior art has low glutathione content, low free radical scavenging ability and tyrosinase activity inhibition intensity, and whitening and anti-oxidation effect when used in cosmetics poor

Method used

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  • Glutathione-rich yeast extract and preparation method thereof as well as application
  • Glutathione-rich yeast extract and preparation method thereof as well as application
  • Glutathione-rich yeast extract and preparation method thereof as well as application

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preparation example Construction

[0031] In the preparation method of glutathione-rich yeast extract of the present invention, the reduced glutathione can be quickly released from the cells through high-pressure homogeneous wall breaking first, because the reduced glutathione is easily absorbed by the body. Source enzyme decomposition, compared with autolytic wall breaking, the use of high-pressure homogeneous wall breaking can retain as much reduced glutathione as possible; Yeast cell wall contains mannan, β-glucan and protein, etc. The inventors found through experiments that two or more of cellulase, glucanase and mannosidase can be used to make mannan and β-glucan degradation to separate glutathione from the yeast cell wall. Since glutathione is easily degraded by protease, compared with protease degradation, the compound enzyme of the present invention can retain more reduced glutathione in yeast and improve the anti-oxidation and whitening effect of yeast extract.

[0032] The present invention also pro...

Embodiment 1

[0054] Yeast preparation method:

[0055] The yeast strain is a yeast strain (saccharomyces cerevisiae) rich in reduced glutathione, and the preservation number is CCTCC M 205130. The nutrient solution is 1% of yeast extract, 2% of peptone and 2% of glucose. For glutathione yeast strains, the normal culture temperature is 30°C. During the fermentation process, the nutrient solution is added in a fed-batch manner, and the first stage is cultivated for 6 hours; the flow rate of the nutrient solution added in the second stage is 20% of the flow rate added in the first stage. Cultivate for 8h. After the cultivation, the fermentation broth is separated to obtain yeast.

[0056] In the batching tank, the yeast is prepared into 10% (yeast dry matter content) yeast milk, the pH is adjusted to 3.0, the temperature is raised to 30° C., and then the homogeneous wall breaking treatment is carried out, and the homogeneous pressure is 50 MPa. Put the above solution into a reaction tank, a...

Embodiment 2

[0058] Yeast preparation method is the same as embodiment 1.

[0059] In the batching tank, the yeast prepared above was formulated into 15% (yeast dry matter content) yeast milk, adjusted to pH = 7.0, cooled to 4°C, and then subjected to homogeneous wall breaking treatment with a homogeneous pressure of 5 MPa. Put the above solution into a reaction tank, adjust the pH=1.0, raise the temperature to 10° C., add 0.05% cellulase and 0.05% mannosidase, and perform enzymatic hydrolysis for 15 hours. Then heat up to 70°C, keep warm for 3 hours to inactivate the enzyme, then centrifuge the enzymolysis solution to take the supernatant, and then use the multi-effect evaporation process to concentrate the above supernatant to a dry matter concentration of 60% to obtain a paste rich in glutathione Glycerin yeast extract, the evaporation temperature is 65°C.

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Abstract

The invention relates to a glutathione-rich yeast extract and a preparation method thereof as well as application. A glutathione-rich yeast raw material is subjected to high-pressure homogenization wall breaking to obtain yeast cell wall milk, then composite enzymatic hydrolysis, enzyme deactivation treatment and centrifugal separation are conducted, and supernate is taken to obtain the glutathione-rich yeast extract. The content of reduced glutathione reaches 5% or above, the content of protein reaches 35% or above, the DPPH free radical clearance rate is 15-20%, the tyrosinase median inhibitory concentration IC50 is smaller than or equal to 0.3 mg/L, and the product has medium antioxidant activity, and can inhibit the dark pigment state and free radical formation caused by tyrosinase overexpression in a body, so that skin color is lightened and the whitening and anti-oxidation effects are achieved.

Description

technical field [0001] The invention relates to the field of yeast extract, in particular to a glutathione-rich yeast extract and its preparation method and application. Background technique [0002] Yeast extract is rich in functional components such as glutathione, other oligopeptides and amino acids, and is widely used in food seasoning, beverages and other industries. Among them, glutathione containing cysteine ​​residues of sulfhydryl groups is the biological major intracellular antioxidants. [0003] At present, the production process of yeast extract can be roughly divided into: autolysis, enzymatic hydrolysis, solid-liquid separation, clear liquid high-temperature concentration and other processes, to obtain extract products with special flavor and color, containing a certain amount of peptides and amino acids . The yeast extract prepared by the prior art can promote the formation of Maillard reaction in the production process to improve the flavor of the product, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/9728A61K8/64A61Q19/02A61Q19/08
CPCA61K8/9728A61K8/64A61Q19/02A61Q19/08A61K2800/782A61K2800/805A61K2800/85
Inventor 肖明华覃先武刘向军李沛李库许琦彭颖李志刚
Owner ANGELYEAST CO LTD
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