The invention discloses a composition for treating and relieving acne, a preparation method and an application. The composition comprises coated yeast fermentation product filtrate, ammonium glycyrrhetate, salicylic acid, frankincense essential oil, prinsepia utilis royle extracts, coated saccharomycetes fermentation product filtrate, Indian chrysanthemum flowers, wild honeysuckle immature flowers, common selfheal spikes, Baikal skullcap roots, deltoid goldthread rhizomes, Mongolian dandelion herbs, weeping forsythia fruits, spreading hedyotis herbs, red-rooted salvia roots, lime fruit peels,thymeleaf rhododendron herbs, Spilanthes oleracea, subshrubby peony flowers, ural licorice roots, bal loonflower roots, Chinese dodder seeds, wormwoodlike motherwort herbs, radices trichosanthis, sessile stemona roots, Chinese trumpetcreeper flowers, Tokyo violet herbs, uniflower swisscentaury roots, rugose rose flowers, Chinese rose flowers, common peony roots, cablin potchouli herbs, divaricatesaposhnikovia roots and common lophatherum herbs. The component has the functions of whitening skin, moisturizing, resisting inflammation and restraining sebum secretion, propionibacterium acnes increase can be restrained, so that the acne is treated and relieved, adverse reaction of glucocorticoid medicines is omitted, quick and long-lasting stimulation is low, and recurrence rate is low.