Preparation of sulfated mannose glucuronic acid hydrogel and application thereof
A glucuronic acid and sulfation technology, applied in bandages, medical science, etc., can solve the problems of ineffective control of wound infection, long inflammation period at wound, and non-healing of wound, etc. It achieves excellent air permeability, large specific surface area, and no Immunogenic effects
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Embodiment 1
[0023] Example 1, preparation of sulfated mannoglucuronic acid hydrogels with different concentrations.
[0024] (1) Take by weighing 10g of sulfated mannose glucuronic acid, dissolve in pure water, stir to make 10% aqueous solution;
[0025] (2) Weigh a certain amount of polyvinyl alcohol (PVA) 1799 type (alcoholysis degree 98-99%), dissolve in pure water in an oil bath at 95°C, stir evenly to make a 10% aqueous solution; in 100mL 10% PVA solution Add the sulfated mannose glucuronic acid solution prepared in (1) dropwise and stir evenly, then put it in a gradient cooling box and place it in a -80°C refrigerator, and control the reaction time for 8-12 hours; after the reaction, put the gradient cooling box at room temperature , control the reaction time 8-12h; take this as a cycle, repeat 2-5 times.
[0026] Note that the final concentrations of sulfated mannoglucuronic acid are 0.1%, 0.5%, 2%, and 5% samples are FU1, FU2, FU3, and FU4, respectively.
Embodiment 2
[0027] Example 2, Structural Characterization of Sulfated Mannoglucuronic Acid Hydrogel
[0028] The contents of fucose and sulfate groups in the raw material sulfated mannoglucuronic acid in Example 1 were characterized by spectrophotometer. The content of fucose in the sulfated mannoglucuronic acid is 28.86%, the content of sulfate groups is 27.06%, and the molecular weight is 8.7×10 4 . Utilize scanning electron microscope to characterize the microscopic appearance of hydrogel FU2 in embodiment 1, scanning electron microscope photo sees figure 1 .
[0029] The obtained hydrogel has a three-dimensional network structure, and it can be seen through a scanning electron microscope that its microstructure contains a large number of pores, which is beneficial for it to absorb wound exudate and gas to pass through.
Embodiment 3
[0030] Example 3, the physical properties of sulfated mannoglucuronic acid hydrogel.
[0031] This experiment evaluated the physical and chemical properties of sulfated mannoglucuronic acid hydrogel by testing the water retention, swelling, and release of uronic acid in liquid medium.
[0032] Test sample: different uronic acid final concentration hydrogel samples FU1-FU4 in embodiment 1
[0033] experiment method:
[0034] Water retention: Take a certain amount of FU1-FU4 hydrogels, put them in a 37°C incubator, and record their quality at regular intervals, with 7 parallel samples in each group.
[0035] Swellability: Take a certain mass of dried FU1-FU4 hydrogel, add enough water and place it in a 37°C incubator, record its mass after fully swelling, and calculate the swelling rate, with 7 parallels in each group.
[0036] Release of sulfated mannoglucuronic acid: Take a certain amount of FU1-FU4 hydrogel and soak it in a certain amount of physiological saline according t...
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