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Microbial compound with effect of relieving irritable bowel syndrome as well as preparation method and application thereof

A technology for irritable bowel syndrome and microbiology, which is applied in the field of microbiological technology and can solve problems such as interference with daily life of patients

Inactive Publication Date: 2021-09-10
JIANGSU WECARE BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

IBS generally does not threaten the patient's life, but it can seriously interfere with the patient's daily life

Method used

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  • Microbial compound with effect of relieving irritable bowel syndrome as well as preparation method and application thereof
  • Microbial compound with effect of relieving irritable bowel syndrome as well as preparation method and application thereof
  • Microbial compound with effect of relieving irritable bowel syndrome as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example 1

[0050] (1) Preparation of BLa80 freeze-dried powder:

[0051] Inoculate the BLa80 strain into the medium according to the inoculum amount accounting for 3% of the total mass of the medium, and culture it at 37°C for 18 hours to obtain a culture medium; centrifuge the culture medium to obtain bacteria; resuspend the bacteria with a lyoprotectant (The mass ratio of the lyoprotectant to the bacteria is 2:1) to obtain a resuspension; the resuspension is lyophilized by a vacuum freezing method to obtain a BLa80 lyophilized powder.

[0052] (2) Preparation of LA85 freeze-dried powder:

[0053] Inoculate the LA85 strain into the medium according to the inoculum amount accounting for 3% of the total mass of the medium, and culture it at 37°C for 18 hours to obtain a culture medium; centrifuge the culture medium to obtain bacteria; resuspend the bacteria with a lyoprotectant (The mass ratio of the lyoprotectant to the bacteria is 2:1) to obtain a resuspension; the resuspension is lyop...

Embodiment 1

[0061] Effects of microbial complexes on colon function in rats with irritable bowel syndrome:

[0062] (1) Grouping situation:

[0063] 50 male SD rats (6 weeks old, body weight between 170g-190g), 3-4 per cage, the feeding temperature was strictly controlled at 22±2°C, the light / dark cycle was 12h, and the rats had free access to food and water. After 2 weeks of adaptive feeding, the rats were randomly divided into 5 groups, 10 rats in each group, which were normal control group, model group, probiotics intervention group I, probiotics intervention group II, and probiotics intervention group III.

[0064] (2) Establish a rat model of irritable bowel syndrome:

[0065] The D-IBS rat model was established by senna leaf gavage combined with restraint stress method. In the third week, all rats were fasted for 12 hours, and the rats in the other groups except the normal group were restrained every morning, and the rats were placed on their backs. Place them on a wooden frame, f...

Embodiment 2

[0074] Effects of microbial complexes on visceral sensitivity in rats with irritable bowel syndrome:

[0075] The grouping situation, the establishment of the irritable bowel syndrome rat model and the experimental process were the same as those in Example 1.

[0076] The minimum volume threshold of the abdominal withdrawal reflex (AWR) induced by balloon colorectal dilatation was evaluated to reflect its visceral sensitivity. Under chloral hydrate anesthesia in rats, a catheter lubricated with paraffin oil was inserted through the anus, and the end of the balloon was 1 cm away from the anus. The catheter and the base of the tail of the rat were wrapped together with adhesive tape to fix the balloon. After waking up, the rats were placed in a special transparent plastic cage, and the rats could only move back and forth in the cage. After a 30-minute adaptation period, 26°C normal saline was injected into the balloon through the external port of the catheter to expand the inte...

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Abstract

The invention relates to a microbial compound with an effect of relieving irritable bowel syndrome as well as a preparation method and application thereof. The microbial compound with the effect of relieving irritable bowel syndrome comprises animal bifidobacterium subsp. Lactis and lactobacillus acidophilus. The invention creatively develops a new strategy for preventing, relieving or treating the irritable bowel syndrome. The microbial compound can obviously improve the intestinal running function of a patient with the diarrhea-type irritable bowel syndrome, obviously reduce the visceral high sensitivity, obviously reduce the levels of serum inflammatory factors including TNF-alpha, IL-6 and IL-17, obviously improve the level of a serum inflammatory factor IL-10, obviously reduce the levels of serum 5-HT and SP, and obviously improve the colon SERT level. The microbial compound has a great application prospect in the preparation of medicines, foods or health-care products for preventing, treating and relieving the diarrhea-type irritable bowel syndrome.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and relates to a microbial compound with the effect of relieving irritable bowel syndrome and its preparation method and application, in particular to a microbial compound with the effect of alleviating irritable bowel syndrome, its preparation method and Application in preparation of medicine for preventing and / or treating irritable bowel syndrome, health food for alleviating irritable bowel syndrome and solid drink for alleviating irritable bowel syndrome. Background technique [0002] Irritable bowel syndrome (IBS) is one of the most common functional gastrointestinal disorders, characterized by abdominal pain or discomfort accompanied by changes in bowel habits and / or abnormal stool consistency. Epidemiological studies show that it affects about 15-20% of adults worldwide. IBS generally does not threaten the patient's life, but it can seriously interfere with the patient's daily life....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/747A61P1/00A61P1/12A23L33/135A23L2/39C12N1/20A61K35/745C12R1/23C12R1/01
CPCA61K35/745A61K35/747A61P1/00A61P1/12A23L33/135A23L2/39C12N1/20A23V2002/00A61K2300/00A23V2200/3204
Inventor 方曙光盖忠辉朱建国王桂龙张秋玉
Owner JIANGSU WECARE BIOTECHNOLOGY CO LTD
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