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Method for efficiently preparing melanin under photo-induction

A melanin, light-induced technology, applied in chemical instruments and methods, azo dyes, organic dyes, etc., can solve the problems of difficult purification, cumbersome and time-consuming melanin production process, etc.

Active Publication Date: 2021-09-10
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a method to solve the current melanin production process is cumbersome, time-consuming, difficult to purify and other problems

Method used

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  • Method for efficiently preparing melanin under photo-induction
  • Method for efficiently preparing melanin under photo-induction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Take 0.05g L-tyrosine as raw material;

[0032] (2) Using 0.005g terpyridyl ruthenium chloride (Ru(II)) and 0.005g potassium persulfate (KPS) as the photoinitiating system;

[0033] (3) Dissolve the photoinitiating system and L-tyrosine in 100mL deionized water sequentially, sonicate until clear and transparent, and then irradiate with a 50W 465nm blue LED for 10min to obtain a reaction mixture;

[0034] (4) Centrifuge the above reaction mixture at a temperature of 20° C., a rotation speed of 10,000 r / min, and a time of 20 minutes. Remove the centrifuged supernatant, and wash and filter the centrifuged solid precipitate 3 times with absolute ethanol. The filtered product was dried at 60°C to finally obtain melanin with a yield of 50.3%.

Embodiment 2

[0036] (1) Take 2.0g L-phenylalanine and 1.0g L-tryptophan as raw materials;

[0037] (2) With 0.001g (2,2'-bipyridyl)bis[2-(2,4-difluorophenyl)pyridine]iridium(III) hexafluorophosphate (Ir(III)) and 0.01g persulfuric acid Ammonium (APS) is the photoinitiating system;

[0038] (3) Dissolve the photoinitiating system, L-phenylalanine and L-tryptophan in 100mL of deionized water in turn, sonicate until clear and transparent, and irradiate under a 100W 350-800nm ​​xenon lamp (with filter) 10min obtains reaction mixture;

[0039] (4) Centrifuge the above reaction product at a temperature of 20° C., a rotation speed of 10,000 r / min, and a time of 20 minutes. The centrifugation supernatant was removed, and the centrifuged solid precipitate was filtered three times with deionized water. The filtered product was dried at 60°C to finally obtain melanin with a yield of 70.7%.

Embodiment 3

[0041] (1) Take 2.0g L-tryptophan, 0.1g L-tyrosine and 20.0g L-alanine as raw materials;

[0042] (2) With 0.0005g camphorquinone (CQ) and 0.05g 4-dimethylamino-ethyl benzoate (EDMAB) as photoinitiating system;

[0043] (3) Dissolve the photoinitiating system and each amino acid in 200mL of deionized water in turn, after ultrasonication until clear and transparent, irradiate for 10 minutes under a 100W 395nm ultraviolet LED to obtain a reaction mixture;

[0044] (4) Centrifuge the above reaction product at a temperature of 20° C., a rotation speed of 10,000 r / min, and a time of 20 minutes. The centrifugation supernatant was removed, and the centrifuged solid precipitate was filtered three times with deionized water. The filtered product was dried at 60°C to finally obtain melanin with a yield of 73.3%.

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Abstract

The invention discloses a method for efficiently preparing melanin under photo-induction. According to the method, efficient preparation of melanin is realized under photo-induction by taking any one or more of L-tyrosine, L-phenylalanine, L-tryptophan, 3, 4-dihydroxyphenylalanine, L-arginine and L-alanine as reaction precursors and any one of transition metal complex / persulfate, thioxanthone or dye / tertiary amine as a photo-initiation system. A photo-initiation system and an amino acid reaction precursor are mixed, and the mixture is irradiated under light with a specific wavelength to react for a certain time to obtain a reaction product; and the product is further centrifuged, filtered, dried, the residual photoinitiator, unreacted amino acid and the like are removed, and finally a melanin product is obtained. Compared with the prior art, the method is simple in preparation process, convenient to operate, high in efficiency and low in cost.

Description

technical field [0001] The invention belongs to the field of melanin preparation, and more specifically relates to a method for photo-induced and efficient preparation of melanin. Background technique [0002] As a high-molecular protein biopigment, melanin usually exists in the skin or hair of animals, as well as plants and protists in a polymerized form. Its content and distribution directly affect the color of skin, hair and eyes. Melanin is mainly composed of two quinone-configured polymers, namely melanin and pheomelanin. The apparent color of melanin is brown or black; the apparent color of pheomelanin is yellow or reddish brown. Melanin effectively protects us from UV radiation, viruses and toxic metal ions. Melanin has a wide range of applications and economic value in medicine, agriculture, food, cosmetics and other industries, so it is of great significance to research and develop synthetic melanin. [0003] Studies on the biosynthesis of melanin have shown tha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09B59/00
CPCC09B59/00
Inventor 黄益孙广东姚菊明李永强邵建中吕玲玲潘小鹏
Owner ZHEJIANG SCI-TECH UNIV
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