Unlock instant, AI-driven research and patent intelligence for your innovation.

Polypeptide for enhancing osteoblast activity and application of polypeptide in treatment of orthopedic diseases

A technology of host cells and uses, application in the treatment of orthopedic diseases, the field of polypeptides that enhance the activity of osteoblasts

Active Publication Date: 2021-09-14
BEIJING ZEQIN BIOMEDICAL CO LTD
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no non-hormone-based peptide drugs at home and abroad

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Polypeptide for enhancing osteoblast activity and application of polypeptide in treatment of orthopedic diseases
  • Polypeptide for enhancing osteoblast activity and application of polypeptide in treatment of orthopedic diseases
  • Polypeptide for enhancing osteoblast activity and application of polypeptide in treatment of orthopedic diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0094] Embodiment 1 polypeptide preparation

[0095] Carry out synthesis by liquid phase synthesis method, concrete steps are as follows:

[0096] 1. Synthetic raw materials and related reagents / instruments

[0097] 1) Resin: 2-Chlorotrityl Chloride Resin with a substitution degree of 1.03mmol / g (Tianjin Nankai Synthetic Technology Co., Ltd.);

[0098] 2) Amino acid: purchased from Chengdu Chengnuo, >99%;

[0099] 3) Synthetic reagents: DMF (origin South Korea), DCM (origin South Korea), MEOH (origin Japan), DIEA (Xinde Chemical, 99%), HBTU (Haofan Biotechnology, 99%);

[0100] 4) Deprotection reagent: piperidine (Sinopharm Shanghai Chemical Reagent Company, 99%);

[0101] 5) Detection reagents: phenol reagent (self-made), pyridine reagent (self-made), ninhydrin reagent (self-made);

[0102] 6) Lysis reagent: 95% cutting fluid: TFA (J.T.Baker, 99%), TIS (Shanghai Darui Fine Chemicals, 98%), EDT (Shanghai Darui Fine Chemicals, 98%), anhydrous ether (Shanghai Experimental, Me...

Embodiment 2

[0121] Example 2 Activity Detection of Polypeptides and Derivatives thereof

[0122] 1. Activity detection of polypeptides of different lengths

[0123] 1.1 Synthesis of polypeptides of different lengths

[0124] Synthesize according to the method for embodiment 1.

[0125] 1.2 Cell experiments

[0126] The osteoblast cell line MC-3T3E1 cells were cultured, and after incubation with polypeptides of different lengths at a final concentration of 2 μg / ml for 24 hours, the expression level of ALP gene was detected by QPCR.

[0127] 1.3 Results

[0128] The result is as figure 1 As shown, the combination of six amino acids of PGAPGP is a better combination to enhance the activity of osteoblasts. When 2-3 PGAPGPs are connected in series, there is good activity, and when 4 or more PGAPGPs are connected in series, the activity begins to weaken.

[0129] 2. Peptide activity detection after amino acid extension at both ends of the polypeptide

[0130] 2.1 Synthesis of polypeptides...

Embodiment 3

[0171] The effect of embodiment 3 polypeptide on mouse bone formation

[0172] 1. Materials

[0173] Source of wild-type mice: All mice were of SPF grade, purchased from Beijing Weitong Lihua (Charles River) Experimental Animal Technology Co., Ltd.

[0174] 2. Polypeptide solution preparation: Weigh 1.000 g of sodium hydroxide in a beaker, add a small amount of normal saline to dissolve, then pour it into a 1000 ml volumetric flask, wash the beaker for 3 times, pour all the solution into the volumetric flask, and finally use physiological Dilute with saline to the mark. After shaking well, a 1000mg / mL polypeptide stock solution was obtained. Dilute in equal proportions according to the required concentration when using.

[0175] 3. Drug treatment

[0176] The mice were randomly divided into two groups, the control group and the experimental group. The mice in the control group were injected with normal saline once a week, and the mice in the experimental group were injecte...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
The inside diameter ofaaaaaaaaaa
Degree of substitutionaaaaaaaaaa
Login to View More

Abstract

The invention discloses a polypeptide for enhancing osteoblast activity and application of the polypeptide in treatment of orthopedic diseases. The polypeptide can promote bone formation. The polypeptide disclosed by the invention is stable in chemical property, easy to synthesize in a low-cost and large-scale manner, high in biocompatibility and non-toxic, and can be widely applied to prevention and treatment of orthopedic diseases.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to a polypeptide for enhancing osteoblast activity and its application in treating orthopedic diseases. Background technique [0002] Now, with the rapid development of my country's transportation and industry, there are millions of patients with bone defects caused by trauma and disease every year. Its treatment and rehabilitation are difficult, causing different degrees of loss of labor ability of patients, causing extreme physical and mental pain to the families of patients, and bringing great harm to the society. Bringing serious economic burden is an urgent problem to be solved. [0003] The development and therapeutic targets of existing anti-osteoporosis drugs are almost all focused on bone remodeling (the dynamic balance between bone formation and bone resorption), that is, how to inhibit bone resorption or promote bone formation. Clinically, anti-osteoporosis drugs are also divided...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K7/06C07K7/08C07K14/00C12N15/11A61K38/08A61K38/10A61K38/16A61P19/08A61P19/10
CPCC07K7/06C07K7/08C07K14/00A61P19/08A61P19/10A61K38/00C07K5/0823C07K5/1024C07K5/0806C07K5/1008A61P19/00A61P19/02C12N5/0654C12N2501/998
Inventor 魏化伟张伦杨承刚
Owner BEIJING ZEQIN BIOMEDICAL CO LTD
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com