Surface-enhanced Raman scattering substrate and manufacturing method thereof
A technology of surface-enhanced Raman and manufacturing methods, which is applied in the directions of Raman scattering, instruments, and analytical materials, and can solve problems such as high cost, inapplicability to mass production, and complex manufacturing process of surface-enhanced Raman scattering substrates.
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Embodiment a
[0050] Take a piece of melamine sponge with a size of 5mm×5mm×2mm, soak it in an equal proportion mixed solution of 95% ethanol and ultrapure water, ultrasonically clean it for 60 minutes, take it out, and place it in an oven at 50°C for drying.
[0051] Prepare nano-gold aqueous dispersion: Mix 1mL CTAB solution (0.2M) with 1mL HAuCl4 solution (0.5mM) by magnetic stirring; then add 120μL NaBH4 solution (10mM) and stir for 2min to obtain gold seed solution. Store at 37°C for 30 min; mix 18 mL of CTAB solution (0.2M) and 3.6 mL of HaCl4 solution (5 mM) by magnetic stirring; add 4 μL of AgNO3 (0.1M), 45 μL of HCl (1.2M) and 2.2 mL of ascorbate to the mixed solution Solution (10mM), after the solution is colorless, add 30μL of the previously stored gold seed solution, and stir for 10s, then store at 37°C for at least 12 hours to obtain the gold growth solution; finally, centrifuge the gold growth solution at 10000rpm for 10min , and resuspended with ultrapure water to stop the re...
Embodiment b
[0054] Take a piece of melamine sponge with a size of 5mm×5mm×2mm, soak it in an equal proportion mixed solution of 95% ethanol and ultrapure water, ultrasonically clean it for 60 minutes, take it out, and place it in an oven at 50°C for drying.
[0055] Preparation of nano-gold aqueous dispersion: heat 50 mL HAuCl4 aqueous solution (1 mM) at 125° C., and vigorously stir and reflux. After boiling the solution, quickly add 2ml of sodium citrate solution (wherein the weight percentage of sodium citrate is 1%). The mixture was continuously heated and boiled, stirred for 30 min, and then cooled to room temperature to obtain a nano-gold (nano-gold particle) aqueous dispersion.
[0056] Soak the dried melamine sponge in the nano-gold aqueous dispersion for 10 minutes, take it out, and place it in a 50°C oven to dry to obtain a surface-enhanced Raman scattering substrate (see Figure 4 ); using ultrapure water as a solvent, the configuration concentration is 10 -12 M, 10 -9 M, 10 ...
Embodiment c
[0058] The surface-enhanced Raman scattering substrate obtained in Example a is selected.
[0059] Crush the pepper sample, accurately weigh 2g into a centrifuge tube, add 25ml of methanol-water solution (7:3v / v) and mix in a vortex mixer for 2min, then ultrasonically extract for 30min; The remaining part was dissolved in 2 mL of methanol and filtered through a 0.22 μm aqueous micromembrane to obtain the test solution.
[0060] Pipette 8 μL of the liquid to be tested onto the surface-enhanced Raman scattering substrate, and after drying, perform Raman spectroscopy detection under the aforementioned conditions. Three types of chili powder, chili paste and dried chili were selected as pepper samples, a total of 6 samples were pre-treated and detected by Raman spectrum according to the above method respectively. The results are shown in Figure 8 and Table 1. The comparison of the detection results of six samples by HPLC showed that the rapid qualitative detection results of th...
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