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Bacillus polymyxa high-yield exopolysaccharide culture medium and application method thereof

A technology of Bacillus polymyxa and exopolysaccharide, which is applied in the field of microorganisms to achieve the effects of promoting secretion, shortening the period of fermentation, and improving the efficiency of exopolysaccharide

Active Publication Date: 2021-09-17
南宁汉和生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the development of society, the demand for microbial exopolysaccharides is constantly increasing, and the current production technology and output of exopolysaccharides cannot meet people's needs for microbial exopolysaccharides. Therefore, it is important to increase the production of microbial exopolysaccharides by fermentation. significance

Method used

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  • Bacillus polymyxa high-yield exopolysaccharide culture medium and application method thereof
  • Bacillus polymyxa high-yield exopolysaccharide culture medium and application method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Medium sample 1: including seed medium and fermentation medium;

[0029] Seed medium (according to mass concentration): yeast powder 30g / L, glucose 25g / L, peptone 13g / L, potassium dihydrogen phosphate 4g / L, ammonium nitrate 1g / L, sodium chloride 0.08g / L, heptahydrate Magnesium sulfate 15g / L, ferric chloride 0.07g / L, the rest is water;

[0030] Fermentation medium (by mass concentration): sucrose 60g / L, fermentation regulator 0.55g / L, peptone 35g / L, yeast powder 40g / L, potassium dihydrogen phosphate 4g / L, magnesium sulfate heptahydrate 15g / L, Ammonium nitrate 1g / L, sodium chloride 0.08g / L, ammonium nitrate 1g / L, ferric chloride 0.07g / L, the rest is water;

[0031] The fermentation regulator is formed by mixing two components, A and B, wherein the component is palmitoyl tripeptide-1, component B is asiaticoside, and the mass ratio of components A and B is 3:2.

[0032] Comparative medium 1-1: the difference from medium sample 1 is that there is no fermentation regulator...

Embodiment 2

[0043] Medium sample 2: including seed medium and fermentation medium;

[0044] Seed medium (according to mass concentration): yeast powder 20g / L, glucose 30g / L, peptone 10g / L, potassium dihydrogen phosphate 0.1g / L, ammonium nitrate 0.2g / L, sodium chloride 0.05g / L, Magnesium sulfate heptahydrate 5g / L, ferric chloride 0.05g / L, the rest is water;

[0045] Fermentation medium (according to mass concentration): sucrose 50g / L, fermentation regulator 0.5g / L, peptone 20g / L, yeast powder 35g / L, potassium dihydrogen phosphate 0.5g / L, magnesium sulfate heptahydrate 5g / L , ammonium nitrate 0.2g / L, sodium chloride 0.05g / L, ammonium nitrate 0.2g / L, ferric chloride 0.05g / L, and the rest is water;

[0046] The fermentation regulator is formed by mixing two components, A and B, wherein component A is palmitoyl tripeptide-7, component B is selected from asiaticoside, and the mass ratio of components A and B is 4:1.

[0047] Comparative culture medium 2-1: different from culture medium sample...

Embodiment 3

[0059] Medium sample 3: including seed medium and fermentation medium;

[0060] Seed medium (according to mass concentration): yeast powder 40g / L, glucose 40g / L, peptone 15g / L, potassium dihydrogen phosphate 8g / L, ammonium nitrate 2g / L, sodium chloride 0.1g / L, heptahydrate Magnesium sulfate 10g / L, ferric chloride 0.1g / L, the rest is water;

[0061] Fermentation medium (by mass concentration): sucrose 80g / L, fermentation regulator 0.6g / L, peptone 45g / L, yeast powder 45g / L, potassium dihydrogen phosphate 10g / L, magnesium sulfate heptahydrate 10g / L, Ammonium nitrate 2g / L, sodium chloride 0.1g / L, ammonium nitrate 2g / L, ferric chloride 0.1g / L, the rest is water;

[0062] The fermentation regulator is a mixture of two components A and B, wherein component A is a mixture of palmitoyl tripeptide-1 and palmitoyl tripeptide-7, wherein palmitoyl tripeptide-1 and palmitoyl tripeptide- 7 The mass ratio is 1:1, component B is madecassoside, and the mass ratio of components A and B is 1:2....

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Abstract

The invention relates to the technical field of microorganisms, and particularly discloses a bacillus polymyxa high-yield exopolysaccharide culture medium and a fermentation method. The culture medium comprises a seed culture medium and a fermentation culture medium, wherein the fermentation culture medium comprises a fermentation regulator; the fermentation regulator is formed by mixing a component A and a component B, wherein the component A is one or a mixture of palmitoyl tripeptide-1 and palmitoyl tripeptide-7, and the component B is selected from one or a mixture of asiaticoside and madecassoside, and the mass ratio of the component A to the component B is (4: 1)-(1: 2). The culture medium is applied to bacillus polymyxa fermentation exopolysaccharide, the yield of the exopolysaccharide can be effectively increased, the production period of the exopolysaccharide is short, and the production efficiency is high.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a high-yield extracellular polysaccharide culture medium for bacillus polymyxa and an application method thereof. Background technique [0002] Microbial exopolysaccharides are a type of carbohydrate compound secreted by microorganisms outside the cell wall during growth and metabolism. Some of them attach to the cell wall of microorganisms to form capsules, which are called capsular polysaccharides; some enter the medium to form mucus, which is called mucus. Polysaccharides are products of microbial adaptation to the environment. The structure of microbial exopolysaccharides is more complex than that of plant polysaccharides, and has unique physical and chemical properties and biological activities. Therefore, it has been widely used in various industries. However, with the development of society, the demand for microbial exopolysaccharides is constantly increasing...

Claims

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Application Information

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IPC IPC(8): C12N1/38C12N1/20C12P19/04C12R1/01
CPCC12N1/38C12N1/20C12P19/04
Inventor 苏善高梁承陈成邹林君余义发吴宁邹仕刚闭革林吴志雄
Owner 南宁汉和生物科技股份有限公司
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