Bacillus polymyxa high-yield exopolysaccharide culture medium and application method thereof

A technology of Bacillus polymyxa and exopolysaccharide, which is applied in the field of microorganisms to achieve the effects of promoting secretion, shortening the period of fermentation, and improving the efficiency of exopolysaccharide

Active Publication Date: 2021-09-17
南宁汉和生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the development of society, the demand for microbial exopolysaccharides is constantly increasing, and the current production technology and output of exopolysaccharid

Method used

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  • Bacillus polymyxa high-yield exopolysaccharide culture medium and application method thereof
  • Bacillus polymyxa high-yield exopolysaccharide culture medium and application method thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0027] Example 1

[0028] Medium Sample 1: a seed medium and fermentation medium;

[0029] Seed medium (mass basis): yeast extract 30g / L, glucose 25g / L, peptone 13g / L, potassium dihydrogen phosphate 4g / L, ammonium nitrate 1g / L, NaCl 0.08g / L, heptahydrate magnesium sulfate 15g / L, ferric chloride 0.07g / L, remainder water;

[0030] Fermentation medium (concentration by weight): sucrose 60g / L, fermentation regulator 0.55g / L, peptone 35g / L, yeast extract 40g / L, potassium dihydrogen phosphate 4g / L, magnesium sulfate heptahydrate 15g / L, ammonium nitrate 1g / L, NaCl 0.08g / L, ammonium nitrate 1g / L, ferric chloride 0.07g / L, remainder water;

[0031] The fermentation was adjusted by mixing two components A and B together, wherein component Palmitoyl Tripeptide-1, component B is asiaticoside, components A and B mass ratio of 3: 2.

[0032] Contrast medium 1-1: culture medium samples is not different from a fermentation regulators, other media samples with ba...

Example Embodiment

[0042] Example 2

[0043] Medium samples 2: comprising a seed medium and fermentation medium;

[0044] Seed medium (mass basis): yeast extract 20g / L, glucose 30g / L, peptone 10g / L, potassium dihydrogen phosphate 0.1g / L, ammonium nitrate 0.2g / L, NaCl 0.05g / L, magnesium sulfate heptahydrate 5g / L, ferric chloride 0.05g / L, remainder water;

[0045] Fermentation medium (concentration by weight): sucrose 50g / L, the fermentation was adjusted 0.5g / L, peptone 20g / L, yeast extract 35g / L, potassium dihydrogen phosphate 0.5g / L, magnesium sulfate heptahydrate 5g / L , ammonium nitrate 0.2g / L, NaCl 0.05g / L, ammonium nitrate 0.2g / L, ferric chloride 0.05g / L, remainder water;

[0046] Fermentation was adjusted by mixing two components A and B together, wherein component A is a palmitoyl tripeptide -7, component B is selected from asiaticoside, components A and B mass ratio of 4: 1.

[0047] Contrast medium 2-1: culture medium samples and 2 except that no adjustment...

Example Embodiment

[0058] Example 3

[0059] Medium Sample 3: a seed medium and fermentation medium;

[0060] Seed medium (mass basis): yeast extract 40g / L, glucose 40g / L, peptone 15g / L, potassium dihydrogen phosphate 8g / L, ammonium nitrate 2g / L, sodium chloride 0.1g / L, heptahydrate magnesium sulfate 10g / L, ferric chloride 0.1g / L, remainder water;

[0061] Fermentation medium (concentration by weight): sucrose 80g / L, fermentation regulator 0.6g / L, peptone 45g / L, yeast extract 45g / L, potassium phosphate monobasic 10g / L, magnesium sulfate heptahydrate 10g / L, ammonium nitrate 2g / L, sodium chloride 0.1g / L, ammonium nitrate 2g / L, ferric chloride 0.1g / L, remainder water;

[0062] Fermentation was adjusted by mixing two components A and B together, wherein component A is Palmitoyl Tripeptide-1 and palmitoyl tripeptide mixture -7, wherein Palmitoyl Tripeptide-1 and palmitoyl tripeptide - 7 mass ratio of 1: 1, component B is from asiaticoside, components A and B mass ratio...

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Abstract

The invention relates to the technical field of microorganisms, and particularly discloses a bacillus polymyxa high-yield exopolysaccharide culture medium and a fermentation method. The culture medium comprises a seed culture medium and a fermentation culture medium, wherein the fermentation culture medium comprises a fermentation regulator; the fermentation regulator is formed by mixing a component A and a component B, wherein the component A is one or a mixture of palmitoyl tripeptide-1 and palmitoyl tripeptide-7, and the component B is selected from one or a mixture of asiaticoside and madecassoside, and the mass ratio of the component A to the component B is (4: 1)-(1: 2). The culture medium is applied to bacillus polymyxa fermentation exopolysaccharide, the yield of the exopolysaccharide can be effectively increased, the production period of the exopolysaccharide is short, and the production efficiency is high.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a high-yield extracellular polysaccharide culture medium for bacillus polymyxa and an application method thereof. Background technique [0002] Microbial exopolysaccharides are a type of carbohydrate compound secreted by microorganisms outside the cell wall during growth and metabolism. Some of them attach to the cell wall of microorganisms to form capsules, which are called capsular polysaccharides; some enter the medium to form mucus, which is called mucus. Polysaccharides are products of microbial adaptation to the environment. The structure of microbial exopolysaccharides is more complex than that of plant polysaccharides, and has unique physical and chemical properties and biological activities. Therefore, it has been widely used in various industries. However, with the development of society, the demand for microbial exopolysaccharides is constantly increasing...

Claims

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Application Information

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IPC IPC(8): C12N1/38C12N1/20C12P19/04C12R1/01
CPCC12N1/38C12N1/20C12P19/04
Inventor 苏善高梁承陈成邹林君余义发吴宁邹仕刚闭革林吴志雄
Owner 南宁汉和生物科技股份有限公司
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