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Gastrin 17 latex enhanced turbidimetry determination kit and preparation and use method thereof

A latex-enhanced, gastrin technology, applied in the field of immunological assays, can solve problems such as instability, achieve high automation, save detection time, and avoid complex processes

Pending Publication Date: 2021-09-21
ANHUI DAQIAN BIO ENG LIMITED
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, G-17 is very unstable in isolated blood, and it begins to degrade rapidly after blood collection. In theory, the storage time at 2-8°C is only 3 hours, which means that it must be completed within 3 hours from blood collection to detection, but in actual work Many hospitals are unable to test within 3 hours, so there will be many invalid results less than 1pmol / L clinically

Method used

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  • Gastrin 17 latex enhanced turbidimetry determination kit and preparation and use method thereof
  • Gastrin 17 latex enhanced turbidimetry determination kit and preparation and use method thereof

Examples

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Effect test

Embodiment 1

[0033] A gastrin 17 latex-enhanced turbidimetric assay kit of this embodiment includes reagent R1 and reagent R2 two-liquid components that are independent of each other; wherein, reagent R1 includes 10mmol / L Tris-HCL buffer, 0.1g / L ProClin300; Reagent R2 includes 10mmol / L Tris-HCL buffer, 1% w / v G17 antibody microspheres.

[0034] Specifically, in this example, regarding the preparation of G17 antibody microspheres: take the activated carboxyl microspheres and G17 antibody for coupling, and the coupled carboxyl microspheres are covalently bonded to the G17 antibody, and stabilize present in Tris-HCL buffer.

Embodiment 2

[0036] A gastrin 17 latex-enhanced turbidimetric assay kit of this embodiment includes reagent R1 and reagent R2 two-liquid components that are independent of each other; wherein, reagent R1 includes 100mmol / L PBS buffer solution, 1g / L azide Sodium; the reagent R2 includes 100 mmol / L PBS buffer, 1% w / v G17 antibody microspheres.

[0037] Specifically, in this example, regarding the preparation of G17 antibody microspheres: take the activated carboxyl microspheres and G17 antibody for coupling, and the coupled carboxyl microspheres and G17 antibody are covalently bonded and stabilized. present in PBS buffer.

Embodiment 3

[0039] A gastrin 17 latex-enhanced turbidimetric assay kit according to this embodiment includes reagent R1 and reagent R2 two-liquid components that are independent of each other; wherein, reagent R1 includes 50mmol / L MES buffer 0.5g / L ProClin300; The reagent R2 includes 50 mmol / L MES buffer, 1% w / v G17 antibody microspheres.

[0040] Specifically, in this example, regarding the preparation of G17 antibody microspheres: take the activated carboxyl microspheres and G17 antibody for coupling, and the coupled carboxyl microspheres and G17 antibody are covalently bonded and stabilized. present in MES buffer.

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Abstract

The invention provides a latex enhanced turbidimetric assay kit for gastrin 17. The latex enhanced turbidimetric assay kit comprises a reagent R1 and a reagent R2 which are liquid components and are independent from each other, wherein the reagent R1 comprises 10 to 100 mmol / L of a buffer solution and 0.1 to 1 g / L of a preservative; and the reagent R2 comprises 10-100 mmol / L of a buffer solution and 1% w / v of G17 antibody microspheres. The invention also provides a preparation and use method of the gastrin 17 latex enhanced turbidimetry determination kit. The kit disclosed by the invention has the advantages that (1) the kit is used for detecting the content of gastrin in serum in vitro, so that a complicated process of gastroscope detection is avoided, and the symptoms of atrophic gastritis are quantitatively detected and determined; moreover, the method can be used for a full-automatic biochemical analyzer, and is convenient, time-saving and energy-saving; meanwhile, the preparation of the reagent is also simple and convenient; and (2) under the conditions of high stability and high precision, compared with similar products, the method has higher sensitivity and specificity.

Description

technical field [0001] The invention relates to the field of immunological determination, in particular to a gastrin 17 latex enhanced turbidimetric assay kit and a preparation method thereof. Background technique [0002] Gastrin is an important gastrointestinal peptide hormone, which was first discovered and named by British scholar Edkins in 1905. Gastrin is synthesized and secreted by G cells located in the gastric antrum and proximal duodenal mucosa. Mature gastrin is amidated gastrin, and amidated gastrin includes G17, G34, G14, G6, G52, G71, of which G17 is the main form of gastrin in the gastric antrum, is the main form of gastrin in the blood after a meal. [0003] The level of serum G17 in patients with gastric cancer is related to the lesion site, clinical stage, and histopathological type. Due to the massive destruction of the oxyntic glands and vagus nerve endings by the cancer tissue, the secretion of gastric acid is lost, and the level of serum gastrin incre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/74G01N33/543
CPCG01N33/74G01N33/54313G01N2333/595
Inventor 芮双印席瑞
Owner ANHUI DAQIAN BIO ENG LIMITED