Mutant chlorella strain and application thereof in sewage treatment
A chlorella, sewage technology, applied in biological water/sewage treatment, water/sludge/sewage treatment, grey water treatment, etc., can solve problems such as limiting application, affecting algal photosynthetic system, affecting pollutant removal effect, etc. , to achieve the effect of small volume, high processing efficiency and strong ammonia nitrogen resistance
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Embodiment 1
[0047]Example 1: Acquisition, Identification and Preservation of Chlorella
[0048] 1. Use Chlorella vulgaris CK-1# as the initial strain. The CK-1# was purchased from the Freshwater Algae Culture Collection at the Institute of Hydrobiology (FACHB) of the Type Culture Collection Committee of the Institute of Hydrobiology, Chinese Academy of Sciences, and the number is FACHB-36.
[0049] Inoculate CK-1# in CT medium for enrichment culture, culture to the logarithmic growth phase (cultivation for more than 7d), put the uniform suspended algal liquid in 12 C 6+ Under ion beam (80MeV / u), radiation mutagenesis was carried out at normal temperature and pressure. The chlorella after radiation mutagenesis was inoculated on the improved CT screening medium. The improved CT screening medium is: on the basis of CT medium, ammonium chloride (2.03g / L) is replaced by potassium nitrate, and dipotassium hydrogen phosphate (1.19g / L) and glucose (5.0g / L) are additionally added. ) Prepare hi...
Embodiment 2
[0060] Example 2: Laboratory effect display of chlorella on sewage treatment
[0061] 1. Application demonstration in the treatment of livestock and poultry breeding simulated sewage.
[0062] (1) The main source of livestock and poultry breeding simulated sewage comes from domestic sewage, adding 1.19g / L KH 2 PO 4 After that, adjust the pH value to 7.80~7.90, after high temperature sterilization (121°C, 20min), add NH 4 Cl (2.03g / L) adjusted the concentration of ammonia nitrogen, and the indicators of simulated sewage from livestock and poultry breeding are shown in Table 3.
[0063] Table 3 Initial values of various indicators of livestock and poultry breeding simulated sewage
[0064]
[0065] Bottle the simulated sewage and the diluted biogas slurry under sterile conditions, 150mL per bottle. Each group was inoculated with equal amounts of the initial algae strain CK-1# and the mutant algae strain HPN-3# according to the dry weight content of 200 mg / L, and culture...
Embodiment 3
[0082] Example 3: Demonstration of the effect of chlorella in treating sewage in an integrated photobioreactor
[0083] The invention also provides the application of the chlorella in sewage treatment. Specifically, such as Figure 18 As shown, an integrated photobioreactor is provided. The reactor includes a closed outer chamber 10, an inner chamber 20 is arranged in the outer chamber 10, a stirring device 23 is arranged in the inner chamber 20, and the stirring end (such as a stirring blade) of the stirring device 23 goes deep into the inner chamber 20 for Stir the reactants in the inner chamber 20 evenly, and the other end of the stirring device 23 extends out of the inner chamber 20, and is externally connected with a driving device, such as a motor (not shown in the figure).
[0084] An aeration device 13 is also arranged in the outer chamber 10 and / or the inner chamber 20, and the aeration port of the aeration device 13 is located in the outer chamber 10 or in the inne...
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