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Shampoo for treating less hair and alopecia

A shampoo and hair loss technology, applied in the field of medicine or cosmetics, can solve the problems of insufficient types of medicines and insufficient types of 5α reductase inhibitors, and achieve good application value and the effect of promoting hair growth

Pending Publication Date: 2021-10-15
宜春希宇生物制品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] But at present, there are not enough types of inhibitors for 5α reductase, and the types of drugs to choose from are not rich enough, so it is worth further research

Method used

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  • Shampoo for treating less hair and alopecia
  • Shampoo for treating less hair and alopecia
  • Shampoo for treating less hair and alopecia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Screening of Type II 5α Reductase High Immunogens

[0039] According to the bioinformatics analysis of the amino acid sequence of human and mouse type II 5α reductase, the secondary structure, antigenicity, hydrophilicity, accessibility and flexibility of PCT protein were comprehensively analyzed, and each segment was analyzed and scored. The high-scoring region was used as the B-cell epitope region. Specifically, using technical parameters such as prediction of β-turn angle by Chou&Fasman method, prediction of antigen surface accessibility by Emini method, prediction of protein flexibility by Karplus&Schulz method, protein antigenicity analysis by Kolaskar&Tongaonkar method, protein hydrophobicity analysis by Parker method and Bepipred linear epitope prediction and other technical parameters, type II was obtained. The epitope peptide of 5α reductase; the chemically synthesized epitope peptide is: N-cvsganflgeiiew-C (SEQ ID NO: 1), and cysteine ​​is introduced...

Embodiment 2

[0040] Example 2 Preparation of Type II 5α Reductase Monoclonal Antibody

[0041] For the first immunization, the immunogen synthesized in Example 1 was diluted to 1 mg / ml with 0.01M pH7.4 phosphate buffer, emulsified and mixed with complete Freund's adjuvant, 50 μg of immunogen per mouse. Inoculate by subcutaneous injection in abdomen and back of mice. Every 2 to 3 weeks, the same amount of immunogen was emulsified and mixed with Freund's incomplete adjuvant for booster immunization, a total of 4 times of immunization, and 1 week after the last immunization, blood was collected from the orbit of the immunized mice, and the serum was separated by centrifugation. In the indirect ELISA method, the immunogen is used to coat the microtiter plate, and the immune titer is detected respectively. The mice with relatively high immune titer were screened out, and 100 μg immunogen was injected intraperitoneally on the 10th day of the last immunization for shock immunization, and routine...

Embodiment 3

[0045] Example 3 Antibody identification

[0046] (1) Affinity identification

[0047] Antibody affinity refers to the tightness of the binding of the antibody to the antigenic determinant. The stronger the affinity, the stronger the binding. It is one of the most important indicators for evaluating the properties of antibodies. Using the FortebioOctetK2 instrument of PALL Company, the affinity constant of the antibody was determined by a kinetic method. The result is that the affinity constant of the antibody is 7.2×10-10, which has a good affinity effect.

[0048] (2) Monoclonal antibody specific detection

[0049] The specificity of monoclonal antibody was detected by ELISA indirect assay method, and the immunogenic peptide was used as positive control, and the specificity of monoclonal antibody was identified with 2 μg / ml BSA, type II 5α reductase, and type I 5α reductase. The result is as figure 1 shown.

[0050] From figure 1 It can be seen from the results that th...

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Abstract

The invention provides the shampoo for treating less hair and alopecia, the shampoo contains the monoclonal antibody specifically aiming at 5 alpha reductase and hesperidin, and experiments prove that the shampoo can well promote mouse hair hyperplasia and has a relatively good application value.

Description

technical field [0001] The invention relates to the field of medicine or cosmetics, and more specifically, to a shampoo for treating hair loss and hair loss. Background technique [0002] Alopecia accounts for more and more in clinical proportion, and the form is various. The etiology of hair loss mainly includes androgenetic alopecia and alopecia areata. The hair follicles of patients with androgenetic alopecia are inherently sensitive to androgen, and the metabolism of 5α-dihydrotestosterone can be inhibited due to the accumulation of 5α-dihydrotestosterone in the hair follicles. Alopecia areata is also considered to be related to immune disorders in addition to nervous system disorders, and is an organ-specific disease mediated by inflammatory cells. The treatment of hair loss includes systemic and local treatments. Oral finasteride and external application of minoxidil tincture have achieved definite curative effects, and high-concentration corticosteroids also have de...

Claims

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Application Information

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IPC IPC(8): A61K39/395A61K31/7048A61K9/08A61K47/10A61K9/06A61K47/60A61K47/69A61P17/14A61K8/64A61K8/60A61K8/86A61K8/14A61K8/02A61Q7/00
CPCA61K39/3955A61K31/7048A61K9/08A61K47/10A61K9/06A61K9/0014A61K47/60A61K47/6913A61P17/14A61K8/64A61K8/602A61K8/86A61K8/14A61K8/0216A61Q7/00A61K2800/782A61K2800/82A61K2300/00
Inventor 余兵生杨洋
Owner 宜春希宇生物制品有限公司
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