Real-time fluorescent nucleic acid isothermal amplification detection kit for treponema pallidum 16s RNA, and special primer and probe thereof

Abstract

The invention discloses a real-time fluorescent nucleic acid isothermal amplification detection kit for treponema pallidum 16s RNA, and a special primer and a probe thereof, and belongs to the technical field of biomedical detection. The kit provided by the invention comprises a nucleic acid extracting solution, a detection solution a, a detection solution b and an SAT enzyme solution, the primers and the probes which are more suitable for treponema pallidum 16s RNA detection are optimally designed, and the components are added step by step in the detection process to perform step-by-step reaction, so that rapid and accurate detection of treponema pallidum 16s RNA can be realized, and the kit is high in sensitivity and high in sensitivity. And the amplification product RNA is easy to degrade and does not cause sample cross contamination and environmental pollution.

Description

technical field [0001] The invention belongs to the technical field of biomedical detection, in particular to a real-time fluorescent nucleic acid constant temperature amplification detection kit for Treponema pallidum 16s RNA and its special primers and probes, in particular to specific target capture technology and real-time fluorescent nucleic acid constant temperature amplification detection Primers, probes and related kits used in real-time fluorescent nucleic acid constant temperature amplification detection of treponema pallidum (TP) 16s RNA combined with Simultaneous Amplification and Test (SAT). Background technique [0002] Syphilis is an infectious disease caused by Treponema pallidum, which can be transmitted through direct sexual contact, mother-to-child transmission, or through blood transfusion, breast-feeding, and clothing and objects that syphilis patients come into contact with (such as surface attachments, drinking water, food, etc.) Or its saliva, etc., c...

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Problems solved by technology

[0004] However, the methods disclosed in the above-mentioned documents 1 and 2 are all based on the basic principle of fluorescent PCR, and temperature rise and fall and circulation are required during the PCR reaction process, so the required detection time is long and the efficiency is low. At the same time, a fluorescent quantitative PCR instrument is required. Increased detection cost; in addition, the reaction product of PCR is DNA, which is not easy to degrade, and it is easy to cause sample cross-contamination and pollution of the experimental environment; moreover, although the minimum detection sensitivity of Treponema pallidum in the above literature 1 and literature 2 can reach 400 ~500copies / ml, but still cannot meet the requirements of higher detection sensitivity

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