Application of aspergillus flavus pathogenic gene cdc48 in screening medicines for preventing and treating aspergillus flavus pollution

A disease-causing gene, the technology of Aspergillus flavus, applied in the field of genetic engineering, can solve the problems of unknown pathogenicity and toxicity of Aspergillus flavus, and achieve the effects of shortening the experimental cycle and time, reducing toxicity, and reducing use

Pending Publication Date: 2021-10-15
XUZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no research report on the cdc48 gene in Aspergillus flavus, and its effect on the pathogenicity and toxicity of Aspergillus flavus is currently unknown

Method used

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  • Application of aspergillus flavus pathogenic gene cdc48 in screening medicines for preventing and treating aspergillus flavus pollution
  • Application of aspergillus flavus pathogenic gene cdc48 in screening medicines for preventing and treating aspergillus flavus pollution
  • Application of aspergillus flavus pathogenic gene cdc48 in screening medicines for preventing and treating aspergillus flavus pollution

Examples

Experimental program
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Effect test

Embodiment

[0025] 1. Knockout of cdc48 gene in Aspergillus flavus

[0026] Search the NCBI database (https: / / www.ncbi.nlm.nih.gov / gene / ), and use bioinformatics methods to identify a new gene with unknown function numbered AFLA_002370 (Gene ID: 238502409) in the genome of Aspergillus flavus. The gene is homologous to the cdc48 (YDL126C) gene of Saccharomyces cerevisiae, so it is named cdc48 gene (SEQ ID NO.1), and the Cdc48 protein has the amino acid sequence shown in SEQ ID NO.2. In order to study the function of the cdc48 gene in Aspergillus flavus in the growth, development and toxicity of Aspergillus flavus, the cdc48 gene knockout fusion fragment was constructed in vitro, and the 2466bp DNA homologous fragment in the Aspergillus flavus genome was used by homologous recombination The argB gene fragment of Aspergillus flavus was replaced to knock out the cdc48 gene in the genome of Aspergillus flavus. figure 1 A shows the principle of this gene knockout.

[0027] The specific method...

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Abstract

The invention relates to application of an aspergillus flavus pathogenic gene cdc48 in screening medicines for preventing and treating aspergillus flavus pollution, which comprises the following steps: constructing an aspergillus flavus culture system of a detection group and an aspergillus flavus culture system of a control group, wherein medicines are added to be screened into the detection group; after the detection group and the control group are cultured for a period of time under the same condition, comparing the expression levels of cdc48 genes or Cdc48 proteins in the aspergillus flavus in the detection group and the control group, and if the expression levels of the cdc48 genes or the Cdc48 proteins in the aspergillus flavus in the detection group are lower than the expression levels of the cdc48 genes or the Cdc48 proteins in the control group to a certain extent, taking the medicine to be screened as the potential medicine for inhibiting the pathogenicity of the aspergillus flavus. According to the screening method disclosed by the invention, the inhibition effect of the medicine on the aspergillus flavus only needs to be detected and identified at a transcriptional level or a protein level, and the content of aflatoxin does not need to be detected, so that the experimental period and time are shortened, the consumption of various organic chemical reagents is reduced, the pollution to the environment is reduced, meanwhile, possible poison to experiment operators is also greatly reduced.

Description

technical field [0001] The invention relates to genetic engineering, in particular to the application of the aspergillus flavus pathogenic gene cdc48 in screening and preventing the aspergillus flavus pollution medicine. Background technique [0002] Aspergillus flavus is a filamentous fungus that widely exists in our living environment, and the conidia and sclerotia produced by it are dispersed in the soil. Aspergillus flavus is an important pathogenic fungus, which can infect the lungs and other organs of humans and animals, cause allergic symptoms and invasive aspergillosis, and pose a serious threat to human health. Aspergillus flavus can infect many important crops, such as peanuts, corn, cotton, etc., which can pollute crops before and after harvest, causing huge economic losses to agricultural production around the world. my country is the hardest-hit area for aflatoxin pollution. Aflatoxin contamination has been detected in corn and peanuts stored in many provinces ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895G01N33/68
CPCC12Q1/6895G01N33/68
Inventor 杨坤龙高晓庆罗越耿青如贺晓娜孙文吴佳雯田俊
Owner XUZHOU NORMAL UNIVERSITY
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