Tree aging space-time pattern identification, stumping and rejuvenation and cuttage large-scale breeding method
A time-space and tree technology, applied in the directions of biochemical equipment and methods, botanical equipment and methods, vegetative reproduction, etc., can solve the problem that the rooting effect of cuttings is not very significant, and improve the survival rate and rooting efficiency of cuttings. Easy to use effect
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Embodiment 1
[0153] Example 1: Screening and expression pattern identification of genes with temporal and spatial differences in aging in Ginkgo biloba
[0154] 1. Sampling
[0155] (1) Sampling according to the time dimension: select 20, 200, and 600-year-old ginkgo trees with the same site conditions and good growth conditions, and use a planer or chisel to collect samples of 50 mm×50 mm in size from the same orientation at the base of the trunk at a height of 1.3 m. Immediately transfer to liquid nitrogen for quick freezing, then scrape or cryosection to obtain cambium.
[0156] (2) Sampling according to the spatial dimension: select a 40-year-old ginkgo tree with good growth potential, and use tall branch shears to cut off different heights from the base to the top (at about 25%, 55%, and 85% of the tree height) branches ( figure 1 ), collected the leaves, scraped the vascular cambium with a utility knife, and immediately transferred the leaves and vascular cambium samples to liquid...
Embodiment 2
[0169] Example 2: Screening and expression pattern identification of genes with temporal and spatial differences in aging of Pinus tabulaeformis
[0170] 1. Sampling
[0171] (1) Sampling according to the time dimension: select the basal vascular cambium of 2, 3, 4, 7, 13, 20, 46, 55 and 123-year-old Pinus tabulaeformis with the same geographical location, the same tissue orientation, and the same family.
[0172] (2) Sampling according to the spatial dimension: select 3-5%, 28-32%, 48-52%, 68-72%, 93- 97% of the height of the vascular cambium is material ( Figure 6 ).
[0173] , Preliminary screening of temporal and spatial difference genes in Chinese pine aging by transcriptome sequencing and analysis
[0174] The samples were subjected to transcriptome sequencing, assembly of sequencing results, gene co-expression network analysis, and differential gene analysis and screening. The genes containing the AP2 conserved domain in plants such as radish were sequenced by MEGA...
Embodiment 3
[0183] Example 3 Selection and expression pattern identification of temporal and spatial regulation factors of aging in Populus nigra
[0184] 1. Select regulatory factors for temporal and spatial aging differences
[0185] In broadleaf trees, small molecule microRNAs miR156 It is a classic age molecular marker, which is expressed in the model plant Arabidopsis and many woody plants such as Populus canada miR156 The expression level decreases with age, so the miR156 As regulators of spatio-temporal differences in senescence of broad-leaved trees.
[0186] , Identification of expression patterns of temporal and spatial differential regulators of aging in Populus nigra
[0187] (1) Sampling
[0188] Sampling according to the spatial dimension: Select the 23-year-old Black Populus americana in good growth condition, and take samples at the heights of 4 meters (low position), 8 meters (middle position) and 10 meters (high position) from the ground respectively, and collect sam...
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