Drug based on LDH nanoparticle co-loaded salinomycin and albumin paclitaxel, and preparation method and application of drug
A nanoparticle and salinomycin technology, which is applied in the directions of drug combination, drug delivery, and pharmaceutical formulation, can solve the problems of limited therapeutic effect of a single drug, and achieve the effects of increasing synergistic efficacy, simple preparation method and high encapsulation efficiency.
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Embodiment 1
[0033] Embodiment 1: The method of co-loading salinomycin and nab-paclitaxel based on LDH nanoparticles specifically includes the following steps:
[0034] (1) Preparation of LDH nanoparticles
[0035] a. Weigh 610mg of magnesium chloride hexahydrate and 241.4mg of aluminum chloride hexahydrate, add them into a centrifuge tube filled with 5mL of deionized water according to the molar ratio of 3:1 (0.6 / 0.2M), and dissolve them completely with the aid of ultrasound to make A solution;
[0036] b. Weigh 320 mg of sodium hydroxide and add it into a glass bottle filled with 20 mL of deionized water (4°C), stir at a constant speed, and react at room temperature for 10 min under the protection of argon to obtain solution B;
[0037] c. Quickly add solution A to the fully dissolved solution B and continue to stir for 20 minutes to make Mg 3 Al-LDH mixed solution;
[0038] d. Mg 3 Transfer the Al-LDH mixed solution into a 50mL centrifuge tube, place it in a high-speed refrigerated ...
Embodiment 2
[0045] Embodiment two: The difference between embodiment two and embodiment one is that the input mass ratio of salinomycin and LDH in step (2) is respectively 0.1:1; 0.5:1 and 1:1, as shown in Table 1, the salt The encapsulation rate of salinomycin is as high as 95.66-98.69%, and the upload rate is 8.46-48.50%. On the one hand, it shows that salinomycin is easy to insert into the LDH carrier layer, and the preparation method is simple; on the other hand, it can improve the utilization rate of salinomycin and reduce the salt The waste of the mycin drug in the preparation process is reduced, and the production cost is reduced.
[0046] Table 1 Effect of input mass ratio of different salinomycin and LDH carrier on Sal / LDH nanoparticles
[0047]
[0048] The encapsulation rate (EE%) expression is EE%=(1-C f / C t )×100%, where C f is the amount of free drug; C t is the total amount of drug in the nanoparticle suspension;
[0049] Loading rate (DL%) refers to loaded drug / (t...
Embodiment 3
[0051] Embodiment 3: The difference between embodiment 3 and embodiment 1 is that the addition amounts of Sal / LDH nanoparticles, nab-paclitaxel Nab-PTX and bovine serum albumin are different.
[0052] Table 2 Effects of different component additions on Sal / LDH@Nab-PTX nanomedicine
[0053]
[0054]
[0055] In the examples in Table 2, the content of Sal / LDH@Nab-PTX nanomedicine components is different by changing the input amount of raw materials, which directly affects the uniformity and potential of the nanoparticle size, thereby affecting the drug delivery effect, for example, when When the amount of serum albumin input is insufficient, Sal / LDH@Nab-PTX (Sal=2.35%; Nab-PTX=2.78%; LDH=25.43%, BSA=69.44%) nanomedicine is easy to aggregate into agglomerates, and the dispersion is uneven, affecting Subsequent dosing. Therefore, after a large number of experiments, the proportions of the components of Sal / LDH@Nab-PTX optimized are: 0.65-3.62% for salinomycin, 0.76-3.71% fo...
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