Pepsinogen multiple detection method and detection kit

Abstract

The invention discloses a pepsinogen multiple detection method and a detection kit. The method comprises the following steps: 1) synthesizing two magnetic coding microspheres with different fluorescence intensities; 2) coupling a PG I capture antibody and a PG II capture antibody on the magnetic coding microspheres; 3) preparing a PG I detection antibody and a PG II detection antibody marked with the same biotin; 4) carrying out immunoreaction; and 5) detecting the magnetic coding microsphere solution obtained in the step 4) by using a flow cytometry, and respectively calculating the concentrations of the pepsinogen PG I and the pepsinogen PG II in the sample to be detected according to the fluorescence intensities of the two magnetic coding microspheres. According to the detection method provided by the invention, the pepsinogen PGI and PGII in a blood sample of a patient can be simultaneously detected, two substances to be detected can be simultaneously analyzed, the operation steps are reduced, the detection error caused by different reagents in an experiment is avoided, the detection time is shortened, and the efficiency and the detection accuracy can be greatly improved.

Description

Technical field [0001] The invention relates to the field of in vitro diagnosis, and in particular to a pepsinogen multiple detection method and detection kit. Background technique [0002] In recent years, gastric cancer has posed a serious threat to human health. With the improvement of people's health care awareness and the advancement of medical methods, although the incidence and mortality of gastric cancer have declined, its incidence rate still ranks at the top of the list of cancer incidence rates in my country. Five, the mortality rate is second only to lung cancer and bronchial cancer. The early clinical symptoms of gastric cancer are not obvious, and most patients with early-stage gastric cancer are not discovered and treated until their disease progresses to an advanced stage. The prognosis of advanced gastric cancer is poor, and the 5-year survival rate of gastric cancer is still low. In Western countries, including Europe and the United States, the 5-year surv...

AI Technical Summary

Problems solved by technology

[0005] Immunoassay is a common detection method for detecting pepsinogen. The current immunoassay technology usually can only detect a single index. Routine laboratories or medical units usually use a single kit to measure the content of pepsinogen in the serum of patients. The detection steps of this method are cumbersome, and there may be various reaction conditions and parameters when the two substances to be detected are not detected at the same time, resulting in inaccurate detection results and detection errors.

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