Pepsinogen multiple detection method and detection kit

A pepsinogen and multiple detection technology, which is applied in the field of pepsinogen multiple detection methods and detection kits, can solve the problems of cumbersome detection steps, detection errors, and inaccurate detection results, so as to shorten the detection time, avoid detection errors, The effect of reducing the number of steps

Pending Publication Date: 2021-10-26
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Immunoassay is a common detection method for detecting pepsinogen. The current immunoassay technology usually can only detect a single index. Routine laboratories or medical units usually use a single kit to measure the content of pepsinogen in the serum of patients. The detection steps of this method are cumbersome, and there may be various reaction conditions and parameters when the two substances to be detected are not detected at the same time, resulting in inaccurate detection results and detection errors.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Pepsinogen multiple detection method and detection kit
  • Pepsinogen multiple detection method and detection kit
  • Pepsinogen multiple detection method and detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A method for multiple detection of pepsinogen provided in this embodiment comprises the following steps:

[0033] 1) Synthesis of two magnetically encoded microspheres with different fluorescence intensities:

[0034] Disperse the magnetic porous microspheres into SDS (sodium dodecyl sulfate) solution and ultrasonically treat it to obtain a uniformly dispersed suspension, then add DVB (divinylbenzene) methanol solution and APC (allophycocyanin) dye, After stirring in a three-neck flask, heat, and after the reaction, magnetically encoded microspheres are obtained, washed with deionized water, separated and dried for later use; among them, two kinds of magnetic beads with different fluorescence intensities are obtained by adding different amounts of APC dyes. Encoded microspheres.

[0035] 2) On the two kinds of magnetically encoded microspheres obtained in step 1), the PGⅠ capture antibody that can bind to pepsinogen PGⅠ and the PGII capture antibody that can bind to pe...

Embodiment 2

[0043] A method for multiple detection of pepsinogen provided in this embodiment comprises the following steps:

[0044] 1) Synthesis of two magnetically encoded microspheres with different fluorescence intensities:

[0045] 1-1) Disperse 10 mg of magnetic polystyrene porous microspheres in 10 ml of SDS (0.25 wt%) solution and ultrasonically disperse for 10 min;

[0046] 1-2) Then add 10mg KPS (potassium persulfate), 40ul DVB methanol solution (1 / 100, V / V), APC (allophycocyanin) aqueous solution (1mg / ml) and ultrasonically disperse for 10min;

[0047] 1-3) Put it into a 25ml three-neck flask and stir for 10 minutes, then gradually raise the temperature to 70°C, and react for 8 hours;

[0048] 1-4) Centrifuge and wash with water for three times, and then dissolve and wash with ethanol for three times through magnetic adsorption separation to obtain APC magnetic fluorescence-encoded microspheres;

[0049] In step 2), two kinds of magnetically encoded microspheres with differen...

Embodiment 3

[0070]This example provides a pepsinogen multiple detection kit, including magnetically encoded microspheres, PGⅠ capture antibody, PGII capture antibody, PGⅠ detection antibody, PGⅡ detection antibody and avidinized phycoerythrin as described in Example 2 The detection kit adopts the detection method as described in Example 2 to detect pepsinogen PGⅠ and pepsinogen PGII.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a pepsinogen multiple detection method and a detection kit. The method comprises the following steps: 1) synthesizing two magnetic coding microspheres with different fluorescence intensities; 2) coupling a PG I capture antibody and a PG II capture antibody on the magnetic coding microspheres; 3) preparing a PG I detection antibody and a PG II detection antibody marked with the same biotin; 4) carrying out immunoreaction; and 5) detecting the magnetic coding microsphere solution obtained in the step 4) by using a flow cytometry, and respectively calculating the concentrations of the pepsinogen PG I and the pepsinogen PG II in the sample to be detected according to the fluorescence intensities of the two magnetic coding microspheres. According to the detection method provided by the invention, the pepsinogen PGI and PGII in a blood sample of a patient can be simultaneously detected, two substances to be detected can be simultaneously analyzed, the operation steps are reduced, the detection error caused by different reagents in an experiment is avoided, the detection time is shortened, and the efficiency and the detection accuracy can be greatly improved.

Description

technical field [0001] The invention relates to the field of in vitro diagnosis, in particular to a pepsinogen multiple detection method and a detection kit. Background technique [0002] In recent years, gastric cancer has posed a serious threat to human health. With the improvement of people's awareness of health care and the advancement of medical methods, although the incidence and mortality of gastric cancer have declined, its incidence is still at the top of the list of cancer incidence in my country Five, the mortality rate is second only to lung cancer and bronchial cancer. The early clinical symptoms of gastric cancer are not obvious, and most patients with early gastric cancer are discovered and treated when the disease progresses to an advanced stage. The prognosis of advanced gastric cancer is poor, and the 5-year survival rate of gastric cancer is still low. In western countries, including Europe and the United States, the 5-year survival rate of gastric cancer...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/573G01N33/543G01N33/533G01N33/532
CPCG01N33/57446G01N33/57488G01N33/573G01N33/54326G01N33/533G01N33/532G01N2333/96477
Inventor 王彤何良南雪燕刘志周白鹏利
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2023 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap