Pepsinogen multiple detection method and detection kit
A pepsinogen and multiple detection technology, which is applied in the field of pepsinogen multiple detection methods and detection kits, can solve the problems of cumbersome detection steps, detection errors, and inaccurate detection results, so as to shorten the detection time, avoid detection errors, The effect of reducing the number of steps
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Embodiment 1
[0032] A method for multiple detection of pepsinogen provided in this embodiment comprises the following steps:
[0033] 1) Synthesis of two magnetically encoded microspheres with different fluorescence intensities:
[0034] Disperse the magnetic porous microspheres into SDS (sodium dodecyl sulfate) solution and ultrasonically treat it to obtain a uniformly dispersed suspension, then add DVB (divinylbenzene) methanol solution and APC (allophycocyanin) dye, After stirring in a three-neck flask, heat, and after the reaction, magnetically encoded microspheres are obtained, washed with deionized water, separated and dried for later use; among them, two kinds of magnetic beads with different fluorescence intensities are obtained by adding different amounts of APC dyes. Encoded microspheres.
[0035] 2) On the two kinds of magnetically encoded microspheres obtained in step 1), the PGⅠ capture antibody that can bind to pepsinogen PGⅠ and the PGII capture antibody that can bind to pe...
Embodiment 2
[0043] A method for multiple detection of pepsinogen provided in this embodiment comprises the following steps:
[0044] 1) Synthesis of two magnetically encoded microspheres with different fluorescence intensities:
[0045] 1-1) Disperse 10 mg of magnetic polystyrene porous microspheres in 10 ml of SDS (0.25 wt%) solution and ultrasonically disperse for 10 min;
[0046] 1-2) Then add 10mg KPS (potassium persulfate), 40ul DVB methanol solution (1 / 100, V / V), APC (allophycocyanin) aqueous solution (1mg / ml) and ultrasonically disperse for 10min;
[0047] 1-3) Put it into a 25ml three-neck flask and stir for 10 minutes, then gradually raise the temperature to 70°C, and react for 8 hours;
[0048] 1-4) Centrifuge and wash with water for three times, and then dissolve and wash with ethanol for three times through magnetic adsorption separation to obtain APC magnetic fluorescence-encoded microspheres;
[0049] In step 2), two kinds of magnetically encoded microspheres with differen...
Embodiment 3
[0070]This example provides a pepsinogen multiple detection kit, including magnetically encoded microspheres, PGⅠ capture antibody, PGII capture antibody, PGⅠ detection antibody, PGⅡ detection antibody and avidinized phycoerythrin as described in Example 2 The detection kit adopts the detection method as described in Example 2 to detect pepsinogen PGⅠ and pepsinogen PGII.
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