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Melittin lipid nanoparticle as well as preparation method and application thereof

A lipid nanoparticle and melittin technology, which is applied in the fields of peptide/protein components, pharmaceutical formulations, and non-active ingredients medical preparations, can solve the problem that nanoparticles cannot achieve targeted drug delivery, and disease treatment is inconvenient and stable. Insufficient sex and other problems, to achieve the effect of convenient treatment, reducing toxic and side effects, and improving specificity

Pending Publication Date: 2021-10-29
上海市宝山区中西医结合医院
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Problems solved by technology

[0004] However, the current melittin drug-loading system still has the following defects: first, many nanoparticles loaded with melittin are not stable enough, and there may be problems of melittin leakage and potential hemolysis; secondly, the existing melittin-loaded nanoparticles Nanoparticles of melittin cannot achieve targeted drug delivery, which brings great inconvenience to the treatment of related diseases

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  • Melittin lipid nanoparticle as well as preparation method and application thereof
  • Melittin lipid nanoparticle as well as preparation method and application thereof
  • Melittin lipid nanoparticle as well as preparation method and application thereof

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Embodiment Construction

[0035] In order to make the technical means, creative features, goals and effects achieved by the present invention easy to understand, the present invention will be further described below in conjunction with specific illustrations.

[0036] A melittin lipid nanoparticle, the melittin lipid nanoparticle is composed of 1,2-dimyristoyl-sn-glycero-3-phosphocholine, cholesterol oleic acid, organic solvent, melittin and inorganic prepared from solvents;

[0037] The percentage mass ratio of above-mentioned each component is:

[0038] 1,2-Dimyristoyl-sn-glycero-3-phosphocholine 10.7-55.5%

[0039] Cholesterol Oleic Acid 1.8-78.7%

[0040] Melittin 0.091-0.7%

[0041] The rest are inorganic solvents.

[0042] Organic solvents are DMSO, DMF, CHCl 3 Any one of them, the inorganic solvent is any one of water and PBS.

[0043] The invention also discloses a method for preparing melittin lipid nanoparticles, comprising the following steps:

[0044] Step 1: Add melittin in a specif...

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Abstract

The invention discloses a melittin lipid nanoparticle. The melittin lipid nanoparticle is characterized by being prepared from 1,2-dimyristoyl-sn-glycerol-3-phosphorylcholine, cholesterol oleic acid, an organic solvent, melittin and an inorganic solvent. According to the melittin lipid nanoparticle, the melittin is effectively carried by adopting the solid lipid nanoparticle, so that the treatment effect of the melittin can be effectively enhanced, the specificity is improved, the drug loading capacity is increased, and the membrane rupture property and hemolytic property of the melittin are lowered; and in addition, targeted drug delivery can be achieved, the bioavailability is improved, and adverse reactions are reduced.

Description

technical field [0001] The invention relates to the technical field of preparation of biomedical materials, in particular to a melittin lipid nanoparticle and its preparation method and application. Background technique [0002] Melittin is a cytolytic polypeptide derived from bee venom. A large number of studies have shown that melittin has strong pharmacological effects and biological activities, and has potential clinical application value in many fields such as anti-inflammation, analgesia, anti-microbial, anti-virus and anti-tumor. Especially in cancer treatment, compared with traditional chemotherapy drugs, melittin is less likely to make cancer cells develop drug resistance. However, melittin can cause severe hemolytic reaction after intravenous injection and damage normal tissues, so the application of melittin in vivo has been greatly limited. [0003] In recent years, with the continuous development of nanotechnology, many nanomaterials, including polymer nanopar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/17A61K9/51A61K47/28A61K47/24A61P11/00
CPCA61K38/1767A61K9/5123A61K9/5192A61P11/00
Inventor 李莉陈瑜丁任杨柳朱融融钱叶长刘志丹李秋红张素芳危蕾芮兴黄婧怡向卉楠
Owner 上海市宝山区中西医结合医院
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