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Photodynamic nano antibacterial material as well as preparation method and application thereof

A nano-antibacterial material and photodynamic technology, applied in antibacterial drugs, nanotechnology, nano-optics and other directions, can solve the problems of inability to ensure the stability of composite materials, inability to meet broad-spectrum antibacterial requirements, and inability of materials to exert antibacterial effects. The effect of aggravating oxidative damage, shortening the action distance, and enhancing the antibacterial effect

Active Publication Date: 2021-10-29
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Current research has found that MoS 2 Combined with organic antibacterial materials by mixing, stirring and other adsorption methods, although the antibacterial activity can be improved to a certain extent, but the stability of the composite material cannot be guaranteed, and the contact between the composite material and bacteria can be reduced, so that the material cannot exert an effective antibacterial effect
In addition, due to the difference in cell structure, chitosan has antibacterial advantages against Gram-negative bacteria, but has weak antibacterial properties against Gram-positive bacteria with thicker cell walls. This difference makes it unable to meet the needs of broad-spectrum antibacterial

Method used

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  • Photodynamic nano antibacterial material as well as preparation method and application thereof
  • Photodynamic nano antibacterial material as well as preparation method and application thereof
  • Photodynamic nano antibacterial material as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A kind of M-CS-Ce6 photodynamic nano antibacterial material, its preparation comprises the steps:

[0040] 50mg MoS 2 Disperse in 25 mL of ultrapure water, sonicate at 20 °C for 6 h, add thioglycolic acid in a volume of 200 μL to the above MoS 2 In the dispersion, sonicate at 25 °C for 24 h to realize the mercapto group in MoS 2 surface modification. Then 65 mg of chitosan was added to the mixture and ultrasonicated for 20 min to form a homogeneous colloidal suspension.

[0041] 150mg EDC and 65mg NHS were gradually added to the mixture, ultrasonicated at 25°C for 15min, stirred at 600rpm at 25°C for 1h to activate the carboxyl group for amide reaction,

[0042] Then the reaction mixture was transferred to a dialysis bag (8 ~ 14KDa), respectively with 10mM acetic acid (1.5 × 10 3 mL) dialyzed for 2 days, ultrapure water (1.5×10 3 mL) was dialyzed for 2 days. Dilute the mixture in 1.2×10 4 Centrifuge at rpm for 30 min, freeze-dry in vacuum at -60°C for 48 h to obt...

Embodiment 2

[0045] A kind of M-CS-Ce6 photodynamic nanometer material, its preparation comprises the steps:

[0046] 35mg MoS 2 Disperse in 25 mL ultrapure water, sonicate at 25 °C for 5 h, add 250 μL volume of thioglycolic acid to MoS 2 In the dispersion, sonicate at 20°C for 20 h to realize the sulfhydryl group in MoS 2 surface modification. Then 70 mg of chitosan was added to the mixture and ultrasonicated for 15 min to form a homogeneous colloidal suspension.

[0047] 70mg EDC and 55mg NHS were gradually added to the mixture, ultrasonicated at 20°C for 12min, and stirred at 660rpm for 50min at 20°C to activate the carboxyl group for amide reaction.

[0048] Afterwards, the reaction mixture was transferred to a dialysis bag (8 ~ 14KDa), and 5mM acetic acid (1.2×10 3 mL) dialyzed for 1.4 days, ultrapure water (1.2×10 3 mL) was dialyzed for 1.4 days. Dilute the mixture at 1.0×10 4 Centrifuge at rpm for 20 min, freeze-dry in vacuum at -70°C for 32 h to obtain M-CS powder, then add ...

Embodiment 3

[0051] A kind of M-CS-Ce6 photodynamic nanometer material, its preparation comprises the steps:

[0052] 25mg MoS 2 Dispersed in 25 mL of ultrapure water, sonicated for 4 h at 22 °C, 167 μL volume of thioglycolic acid was added to the above MoS 2 In the dispersion liquid, sonicate at 22°C for 16h to achieve the 2 surface modification. Then 25 mg of chitosan was added to the mixture and ultrasonicated for 10 min to form a homogeneous colloidal suspension.

[0053] Gradually add 60mg EDC and 25mg NHS to the mixture, sonicate at 22°C for 10min, stir and react at 800rpm for 40min at 22°C to activate the carboxyl group,

[0054] Then the reaction mixture was transferred to a dialysis bag (8 ~ 14KDa), respectively with 5mM acetic acid (1.0 × 10 3 mL) dialyzed for 1 day, ultrapure water (1.0×10 3 mL) was dialyzed for 1 day. Dilute the mixture to 1.1×10 4 Centrifuge at rpm for 15 min, freeze-dry in vacuum at -65°C for 24 h to obtain M-CS powder, then add water and ultrasonicall...

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Abstract

The invention discloses a photodynamic nano antibacterial material as well as a preparation method and application thereof. The photodynamic nano antibacterial material is prepared by loading a photosensitive material on chitosan chemical functionalized MoS2. According to the preparation method disclosed by the invention, after chitosan and MoS2 are covalently bound, a photosensitizer Ce6 is physically adsorbed to the surface of M-CS, and the prepared photodynamic nano antibacterial material shows a broad-spectrum and efficient antibacterial effect and has a wide application prospect in the antibacterial field.

Description

technical field [0001] The invention relates to the field of nanometer materials and microorganisms, in particular to a photodynamic nanometer antibacterial material and its preparation method and application. Background technique [0002] Pathogenic bacteria are an important problem that endangers human life and health. With the abuse of antibiotics, bacteria develop drug resistance, the antibacterial efficiency of drugs decreases, and pathogenic bacteria infections aggravate, causing a series of hazards. The problem of pathogenic bacteria increases the need for new antimicrobial agents. Due to their special size effect and chemical reaction sites, nanomaterials have a unique antibacterial mechanism and are not easy to cause drug resistance, so they have become a research hotspot for new antibacterial agents. Molybdenum disulfide (MoS 2 ), compared with metals and photocatalytic nanomaterials, it has higher specific surface area, loading rate, and good biocompatibility; c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K47/55A61K47/61A61P31/04B82Y5/00B82Y20/00B82Y30/00B82Y40/00A61K33/24
CPCA61K41/0071A61K47/61A61K33/24B82Y5/00B82Y20/00B82Y40/00B82Y30/00A61P31/04A61K47/55A61K2300/00Y02A50/30
Inventor 王周平曹文博乐琳
Owner JIANGNAN UNIV
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