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Strawberry transcription factor for promoting synthesis of polyphenol and triterpene substances and application thereof

A substance, plant polyphenol technology, applied in the fields of genetic engineering and molecular biology, can solve problems such as unclear

Pending Publication Date: 2021-10-29
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, it is difficult to predict and analyze the function of FvMYB17 in strawberry based on the results of FvMYB17 heterologous transformation of Arabidopsis
Whether the FvMYB17 transcription factor can promote anthocyanin synthesis in strawberry or whether the transcription factor can promote the synthesis of other secondary metabolites is still unclear

Method used

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  • Strawberry transcription factor for promoting synthesis of polyphenol and triterpene substances and application thereof
  • Strawberry transcription factor for promoting synthesis of polyphenol and triterpene substances and application thereof
  • Strawberry transcription factor for promoting synthesis of polyphenol and triterpene substances and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1, Obtaining and Phenotype Analysis of FvMYB17 Transgenic Strawberry Plants

[0019] 1.1 Transformation of diploid forest strawberry 'Ruegen'

[0020] (1) Shake bacteria. Pick the correct positive colony and culture it with YEP liquid containing 25 mg / L rifampicin and 100 mg / L kanamycin based on a 28°C constant temperature shaking incubator for 12-16 hours overnight until orange-yellow. The next morning, transfer 1 mL of bacterial liquid to a new 50 mL YEP liquid medium, and continue to cultivate to OD 600 The value is between 0.5-0.6.

[0021] (2) Material selection. Select diploid 'Ruegen' tissue-cultured strawberry seedlings with good growth potential, quickly cut off the periphery of the leaves, leave a 2-4mm wide part, make the cut as flat as possible, and moisten and soak the cut part with co-cultivation liquid medium Save for later.

[0022] (3) Infection. The cut explants were transferred to the bacterial solution, and the infection was started for...

Embodiment 2

[0032] Example 2, Detection of secondary metabolites in the fruit of FvMYB17 transgenic strawberry plants

[0033] The red fruits of the two lines of transgenic plants and wild-type plants were sampled at the same time (at least 6 seedlings for each line), mixed and ground separately, and sent to the company for secondary metabolome testing. Set fold change ≥ 1.5 to perform differential analysis on metabolites, and the results are shown in Table 1. The content of main polyphenolic compounds in the two FvMYB17-overexpressed strawberry transgenic lines was generally significantly higher than that of wild-type plants, and some triterpenoids were found to be significantly higher than that of wild-type plants. It shows that FvMYB17 can increase the content of polyphenols and triterpenoids in strawberry fruit.

[0034] Table 1 Differences in the contents of polyphenols and triterpenoids in the fruits of FvMYB17 transgenic strawberry plants and wild-type control plants

[0035] ...

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Abstract

The invention relates to a strawberry transcription factor for promoting synthesis of polyphenol and triterpene substances and application thereof, belongs to the field of gene engineering in molecular biology, and researches show that the strawberry transcription factor FvMYB17 has the function of promoting synthesis of the polyphenol and triterpene substances in strawberries. The invention provides a basis and a technical means for improving the polyphenol and triterpenoid substances in plants by utilizing a genetic engineering technology, and has a great application value.

Description

technical field [0001] The invention belongs to the technical fields of molecular biology and genetic engineering, and specifically relates to a strawberry transcription factor for promoting the synthesis of polyphenols and triterpenoids and an application thereof. Background technique [0002] Polyphenolic compounds are a complex class of secondary metabolites with multiple phenolic hydroxyl groups. In the whole plant kingdom, there are more than 6,500 kinds of polyphenolic compounds and their derivatives, which are secondary by-products in the process of plant metabolism. . According to the number of aromatic rings in the substance structure and the number of hydroxyl structures connected to it, it can be divided into flavonoid compounds, phenolic acid compounds, stilbene compounds, lignans and polymerized lignin. Flavonoids account for about two-thirds of the total amount of polyphenols ingested by humans, and most of them exist in the form of derivatives, mostly in the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/08A01H6/74
CPCC07K14/415C12N15/8243
Inventor 张俊祥雷莹莹代红艳张志宏
Owner SHENYANG AGRI UNIV