Edible fungus biocontrol ferment fermentation strain, fermentation method and application of edible fungus biocontrol ferment fermentation strain in disease resistance of rice seedling stage

The technology of enzyme fermentation and fermentation method is applied in the field of utilization of edible fungus biocontrol enzyme fermentation strains, which can solve the problems of long fermentation period, natural fermentation and easy pollution.

Pending Publication Date: 2021-10-29
江苏稼润农业开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

No matter which method is used, it is all natural fermentation; enzyme drinks derived from natural fermentation may have the following problems: (1) The fermentation cycle is long; (2) The process is uncontrollable due to factors such as raw materials, ambient temperature, and ambient humidity (3) Natural fermentation, easy to pollute

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Embodiment 1: Fermentation agent is applied to the production of 3 kinds of edible fungus biocontrol enzymes

[0056] Experimental materials: 6 kinds of edible fungi: Flammulina velutipes, Pleurotus chinensis, and succulent milk mushrooms; the ratio of the above three kinds of edible fungi is 3:3:2, totaling 8kg; molasses 2kg; Clostridium cellulosus 100ml, Lactobacillus plantarum fermentation agent and Lactobacillus casei fermentation agent 50ml each.

[0057] Fermentation process: After mixing Flammulina velutipes, Pleurotus chinensis, and succulent milk mushrooms in a ratio of 3:3:2, take 8kg, and then mix it evenly with 2kg of molasses, first add 100ml of Clostridium thermocellum, this strain can In the early stage of edible fungus fermentation, the cellulose and hemicellulose components in the edible fungi are effectively used to quickly generate soluble sugars. When the soluble sugar content in the fermentation liquid reaches 15g / kg, you can add 100ml of Pichia pa...

Embodiment 2

[0058] Embodiment 2: Fermentation agent is applied to the production of 6 kinds of edible fungus biocontrol enzymes

[0059] Experimental materials: 6 kinds of edible fungi: Pleurotus eryngii, Pleurotus chinensis, and Bailing mushroom; Pleurotus eryngii, Branch coral fungus, and succulent milk mushroom The ratio of the above 6 kinds of edible fungi is 4:3:2:1:1 1, 11kg in total; Molasses 3.0kg; 150ml of Pichia pastoris fermentation agent, Clostridium thermocellum 150ml, each 50ml of the fermentation agent of Lactobacillus plantarum and the fermentation agent of Lactobacillus casei.

[0060] Fermentation method: After mixing the above six kinds of edible fungi, Pleurotus eryngii, Pleurotus chinensis, and Bailing mushroom; Take 11kg and mix it evenly with 3.0kg of molasses. First, add 150ml of Clostridium thermocellum. This strain can effectively utilize the cellulose and hemicellulose components in the edible fungi in the early stage of edible fungus fermentation, and can be de...

Embodiment 3

[0065] Example 3: Application of Edible Fungus Biocontrol Enzyme in Rice Seedling Raising and Disease Prevention-Disinfection and Disease Prevention of Substrate Soil

[0066] The base soil before seedling raising is crushed and sieved for later use. Before raising seedlings, spray the edible fungus biocontrol enzyme and seedling raising substrate soil on the substrate soil at a ratio of 1:100 2 hours in advance. Afterwards, the seedlings were raised according to the conventional seedling raising steps. At the same time, the matrix soil without the edible fungus biocontrol enzyme was used as a control.

[0067] After the seedlings are raised, the seedlings sprayed with edible fungus biocontrol enzymes will reduce the disease rate. After high-throughput analysis of microorganisms in sprayed and non-sprayed samples, it was found that the types of flora were not much different, but the dominant flora was quite different. Compared with normal samples, the bacteria of Proteobact...

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PUM

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Abstract

The invention provides an edible fungus biocontrol ferment fermentation strain (strain) capable of inhibiting and preventing diseases of a rice seedling stage. The edible fungus biocontrol ferment fermentation strain is used for fermentation production of edible fungus biocontrol ferment, so that the purpose that the edible fungus is fermented once to produce the biocontrol ferment can be realized, the disease prevention and inhibition functions of the rice seedling stage can be strengthened, the quality guarantee period of products can be prolonged, and the fermentation cost can be reduced. The invention further provides an application of the ferment fermentation strain in fermentation of the edible fungus biocontrol ferment. The invention further provides a fermentation method of the edible fungus biocontrol ferment. The fermentation method provides optimal data guidance for fermentation production of the biocontrol ferment by using the fermentation strain, so that the edible fungus biocontrol ferment having the disease prevention and inhibition functions of the rice seedling stage can be quickly obtained in a short term.

Description

technical field [0001] The invention relates to the technical field of utilization of edible fungus biocontrol enzyme fermentation strains, in particular to the application of edible fungi biocontrol enzyme fermentation strains, fermentation methods and disease resistance in rice seedling stage. Background technique [0002] Agricultural enzymes generally use plants as the main raw material, with or without supplementary materials, and are naturally fermented enzyme products that contain specific biological activities and are used in planting, breeding, and soil improvement. Various nutritional components of agricultural enzymes, such as probiotics, biological enzymes and various nutrients, have important biological functions: probiotics participate in many important life activities, and at the same time produce some biological enzymes and probiotic factors that are beneficial to life activities through their own metabolism Wait. Therefore, in recent years, agricultural enz...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C12P1/02C12P1/04A01N63/20A01N63/32A01P1/00A01P3/00C12R1/145C12R1/25C12R1/225C12R1/84
CPCC12N1/20C12N1/16C12P1/02C12P1/04A01N63/20A01N63/32
Inventor 袁旭峰黄亚明王友琪刘创崔宗均温博婷郭铭男
Owner 江苏稼润农业开发有限公司
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