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Halobacteriovorax vibrionivorans microbial agent and preparation method and application thereof

A technology of phagocytic Vibrio and Vibrio, applied in the cultivation of Penaeus vannamei, Vibrio halophilic phage Vibrio agent and its preparation field, can solve the problem of high cost, time-consuming, strain production process cumbersome and other issues, to achieve the effect of safety cost and low cost

Pending Publication Date: 2021-10-29
HUAQIAO UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, the Bdellovibrio bacteria agents on the market have the following technical shortcomings and deficiencies: the production process of the selected strains is cumbersome and time-consuming; the medium used is rich in nutrients and high in cost; Escherichia coli or Vibrio bacteria are used as production The host has application ecological risks; the storage period is short and the number of viable bacteria is low, so the effect of production and application is far from the theoretical effect

Method used

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  • Halobacteriovorax vibrionivorans microbial agent and preparation method and application thereof
  • Halobacteriovorax vibrionivorans microbial agent and preparation method and application thereof
  • Halobacteriovorax vibrionivorans microbial agent and preparation method and application thereof

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Embodiment 1

[0044] In this embodiment, a vibrio halophilic phage vibrio agent is prepared by the following method:

[0045] In host bacteria containing Bacillus subtilis (10 8-10 cfu / mL) artificial seawater liquid medium was inserted into Vibrio halophilus Vibrio BL9 (10 5 pfu / mL), cultivated at 20-35°C for 24h, added the host again, continued to cultivate for 0.5-1h, added sterilized xanthan gum with a final concentration of 0.05-0.3% (w / v), mixed evenly and divided to obtain Vibrio halophilic phage vibrio agent, the amount of live bacteria reaches (10 8-9 pfu / mL).

[0046] 1. Predation characteristics and lysis spectrum of Vibrio halophilus Vibrio

[0047] The predation characteristics and cleavage spectrum of Vibrio halophilicus were determined by transmission electron microscope observation, dissociation curve method and seawater double-layer plate filter paper method.

[0048] Transmission electron microscope observation method: 100mL Vibrio halophilus Vibrio BL9 and host bacteri...

Embodiment 2

[0062] This example provides the application of a vibrio halophilic vibrio agent in the cultivation of Penaeus vannamei.

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Abstract

The invention discloses a halobacteriovorax vibrionivorans microbial agent and a preparation method and application thereof. According to the halobacteriovorax vibrionivorans microbial agent and the preparation method and application thereof, Bacillus subtilis is used as a prey host, and seawater or artificial seawater is used as a culture medium, the rapid, simple, convenient, safe and low-cost production method of the halobacteriovorax vibrionivorans bacterial agent is provided. The selected host has no environmental risk, the whole production process can be quickly completed within 48 hours, the liquid microbial agent can be preserved for more than 12 months at normal temperature, and the microbial agent can promote growth of penaeus vannamei, reduce the content of vibrio in the intestinal tract of the penaeus vannamei and keep micro-ecological balance in the intestinal tract of the penaeus vannamei in the culture of the penaeus vannamei.

Description

technical field [0001] The invention belongs to the technical field of biotechnology, and in particular relates to a vibrio halophilic phage vibrio agent, a preparation method thereof, and an application in culture of Penaeus vannamei. Background technique [0002] Bdellovibrio-and-like organisms (BALOs) refer to a class of small obligate predatory parasitic Gram-negative bacteria that attack and lyse large bacteria (mainly Gram-negative bacteria), and have phage-like It is widely used in various environments, such as water body, sediment, root system, soil, animal skin surface or intestinal tract, etc. The unique "parasitic" and "bacteriophage" biological characteristics of this type of bacteria have attracted the attention of microbiology scholars, and they also have broad application prospects in aquaculture, water purification, agriculture, and livestock and poultry industries. [0003] In aquaculture, BALOs can be used as "active antibiotics" and have great application...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/04A61K35/74A61P1/10A61P1/12A61P31/04C12R1/01
CPCC12N1/20C12N1/04A61K35/74A61P31/04A61P1/12A61P1/10
Inventor 陈明霞赵炎何小玉叶晓声
Owner HUAQIAO UNIVERSITY
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