Supercharge Your Innovation With Domain-Expert AI Agents!

Process for production of carotenoid

A technology of carotene and its manufacturing method, applied in skin care preparations, chemical instruments and methods, medical preparations containing active ingredients, etc., capable of solving problems such as low simplicity, inefficiency, and solvent residues

Inactive Publication Date: 2013-08-28
NIPPON OIL CO LTD
View PDF10 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0023] In order to solve the above-mentioned problems, the present inventor studies microbial cultures, and finds that trying to obtain high-purity carotenoids by using the liquid-liquid extraction method of the prior art has the following problems: 1) There are many solutions used in the operation steps, so It is industrially inefficient, which is the same as the conventional high-purification and refining technology in a low-concentration solution state; 2) Solvent recovery including fractional operation of mixed organic solvents must be performed, resulting in low simplicity; 3) The solvent used remains in the product, which is poor in safety

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Process for production of carotenoid
  • Process for production of carotenoid
  • Process for production of carotenoid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0130] [Example 1] Production of high-purity carotenoids with high astaxanthin content 1

[0131] Step 1: Cultivation steps of E-396 strain

[0132] Add 10 ml of medium containing 2 g / L glucose, 3 g / L meat extract, 10 g / L peptone, and 5 g / L sodium chloride into a test tube with a diameter of 18 mm, and steam sterilize at 121° C. for 15 minutes. One platinum inoculation loop amount of E-396 strain (FERM BP-4283) was inoculated therein, and cultured with repeated shaking at 30° C. and 300 rpm for 6 days. 200 tubes (2 L) of the culture solution were centrifuged and then freeze-dried to obtain dried bacterial cells containing 16 mg of astaxanthin per 1 g.

[0133] Step 2: Ethanol extraction step

[0134] 18 kg of ethanol was added to 62 g of the dried cells obtained in Step 1 of this example, stirred at 50° C. for 3 hours, and carotenoids containing astaxanthin were extracted. Then, the thalline was removed by filtration, and the thalline filter cake (ケク) was washed with ethano...

Embodiment 2

[0144] [Example 2] Production of Astaxanthin High-Content High-Purity Carotenoid Using Recovered Solvent 1

[0145] In 31g of the dried thalline obtained in step 1 of embodiment 1, add 9 kg of the distillate (ethanol) obtained in step 3 of embodiment 1, and carry out extraction of carotenoids and filtration of thalline according to the method in step 2 of embodiment 1 to obtain the extract 9kg. After concentrating the extract under reduced pressure according to Step 3 of Example 1, about 40 g of the cooled concentrate was obtained. The precipitate was filtered off from the cooled concentrate as in Example 1, step 4. Then, 10 g of the ethanol recovered in Step 6 of Example 1 was added from above the precipitate filter cake for filtration and washing, and then dried to obtain 0.75 g of dried product. Add water-containing 25% ethanol (by the ethanol 37.5g that embodiment 1 step 6 reclaims and water 12.5g mix and make) in this dry thing, obtain water-containing 25% ethanol washi...

Embodiment 3

[0147] [Example 3] Production of high-purity carotenoids with high astaxanthin content 2

[0148]Bacterial culture, extraction, concentration and precipitation steps are carried out according to the same method as step 1 to step 3 of embodiment 1, and further according to step 4 of embodiment 1, the cooling concentrate obtained in step 3 is filtered under reduced pressure about 80g to obtain carotenoid-containing sediment filter cake. Further, 20 g of ethanol was added from above the filter cake to filter and wash, and then, a part of the dry filter cake was taken to investigate the carotenoid content of the precipitate. Then, the drying step was omitted, and 100 g of ethanol containing 25% water was added from above the filter cake for further filtration and washing. The filtered and washed cake was dried overnight at 35°C under reduced pressure to obtain 1.5 g of a dried product containing water and 25% ethanol. In the vacuum-dried product before washing with 25% ethanol c...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

Disclosed is a process for production of a composition having a carotenoid content of 80% or more, which comprises: extracting a culture of a microorganism, a condensation product of the culture or a dried product of the culture or the condensation product with at least one member selected from the group consisting of a lower alcohol, a water-containing lower alcohol, a lower dialkyl ketone and an ether; condensing the resulting extract to yield a precipitate; and washing the precipitate with at least one member selected from the group consisting of a lower alcohol, a water-containing lower alcohol, a hydrocarbon solvent and a lower dialkyl ketone. Also disclosed is a food, a pharmaceutical composition or a cosmetic comprising the carotenoid-containing composition.

Description

technical field [0001] The invention relates to a method for preparing carotenoids, in particular to a method for preparing astaxanthin suitable for industrial production. Astaxanthin is an ingredient used in food, pharmaceutical compositions or cosmetics. In addition, the present invention also relates to the carotenoid-containing composition obtained by the above method, and foods, pharmaceutical compositions or cosmetics containing the carotenoid-containing composition. Background technique [0002] Carotenoids are natural pigments that widely exist in nature, and are polyene pigments that exhibit various colors from yellow to red or purple. Astaxanthin is one of the natural carotenoids found, and it exists not only in the free state or in the form of esters, but also in the form of various pigment proteins bound to proteins. [0003] Astaxanthin is widely used as a color enhancer for fish and eggs. In addition, it is also used as a food additive, and is widely used in ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07C403/24B01D11/02A23L1/30C09B61/00A61K8/35C12P23/00A61K31/122A61K8/99A61P17/18A61K35/74A61P39/06C12N15/01A61Q19/00A23L33/10
Inventor 石崎友幸石川觉坪仓章平泽和明
Owner NIPPON OIL CO LTD
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com