Intra-ovum injection for improving salmonella resistance of newborn poultry and application

A Salmonella and injection technology, applied in the field of intra-ovo injection, can solve the problems of slow onset of traditional Chinese medicine, residual antibiotics, adverse reactions, etc., to improve the ability to resist Salmonella, improve the speed of maturation, and no drug residues.

Pending Publication Date: 2021-11-02
PANZHIHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Conventional treatment of Salmonella infection in chicks is mainly based on antibiotic therapy. However, with the continuous development of the poultry industry, the pathogenic mechanism of Salmonella infection is becoming more and more complex, and the antibiotic resistance of pathogenic bacteria is becoming stronger and stronger. Therefore, the effect of antibiotic treatment alone gradually weakening
Antibiotic treatment of Salmonella will lead to drug residues in poultry and egg products, which will induce adverse reactions after human consumption
Antibiotics will also remain in poultry feces, which will cause environmental pollution after being excreted, causing soil and water antibiotics to exceed the standard
[0005] It is also not feasible to prevent Salmonella infection in chicks with vaccines. First, Salmonella mainly infects chicks with immature immune systems. After vaccination, chicks cannot produce immune responses and antibodies. Therefore the vaccine is less likely to improve chick resistance to Salmonella infection
[0006] At present, traditional Chinese medicine preparations are also used to prevent salmonella infection in chickens. Among them, astragalus extract or astragalus compound traditional Chinese medicine has the greatest potential. Infection within weeks of age and avoidance of morbidity and mortality in chicks

Method used

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  • Intra-ovum injection for improving salmonella resistance of newborn poultry and application
  • Intra-ovum injection for improving salmonella resistance of newborn poultry and application
  • Intra-ovum injection for improving salmonella resistance of newborn poultry and application

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preparation example Construction

[0053] The present invention also provides a preparation method of the injection, which includes the following steps: heating and dissolving the above-mentioned active ingredient in the above-mentioned injection carrier under the condition of avoiding light, and then storing it in the dark after being sterilized.

[0054] Further, the present invention also provides the preparation method of the injection, comprising the following steps: adding astragalus polysaccharide and / or its pharmaceutically acceptable salt and / or ester at a concentration of 0.5-10% at 65°C in a dark environment Heat and dissolve in sterile DMEM cell culture fluid, or a pharmaceutically acceptable diluent for embryo or cell injection including glucose solution and / or PBS buffer, and then sterilized and stored away from light.

[0055] The present invention also provides a storage method for the injection, which includes the following steps: the injection is stored at -20°C for no more than 3 months or at 4°...

Embodiment 1

[0065] Embodiment 1 (preparation of injection of the present invention)

[0066] Solarbio’s 1g of astragalus polysaccharide dry powder is dissolved in 100g of sterile DMEM cell culture liquid at a concentration of 1%, heated and dissolved in sterile PBS buffer at 65°C, and then stored in the dark after sterilization.

[0067] Sterile DMEM cell culture fluid, glucose solution, and PBS buffer have the same function and effect in injection, and can be replaced with each other.

Embodiment 2

[0068] Embodiment 2 (the usage method of injection of the present invention)

[0069] Take SPF eggs or chicken embryos that have been hatched to 16 days old, and determine the position of the yolk sac at the small head of the chicken embryo according to the egg; after disinfecting the surface of the eggshell with 75% alcohol, drill a hole at the position of the yolk sac with a 1mm diameter micro electric drill; take out the egg from the refrigerator Invented injection, preheated at 37.5°C for 5 minutes before injection; took a 1mL syringe with a diameter of 0.7mL to draw the injection, and inserted it into the yolk sac of a chicken embryo through a hole at the small end, with an injection depth of 15mm and an injection dose of 0.5mL; Seal; each egg injection operation time does not exceed 5 minutes; in order to avoid cross-infection between eggs, each egg uses a separate syringe.

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Abstract

The invention belongs to the technical field of agricultural animal husbandry production, and particularly relates to an intra-ovum injection for improving the salmonella resistance of newborn poultry and the application of the intra-ovum injection. The technical problem to be solved by the invention is to provide the in-ovum injection which is used for poultry embryos, has small toxic and side effects and no drug residue and is used for preventing newborn poultry from being infected with salmonella and the application of the intra-ovum injection. The intra-ovum injection comprises the following active ingredient: astragalus polysaccharide. The injection provided by the invention can effectively improve the development and maturation speed of immune organs of newborn poultry and improve the capability of resisting salmonella of poultry infected within one week old, and has a better effect of preventing infection of newborn poultry than conventional astragalus drugs.

Description

technical field [0001] The invention belongs to the technical field of agricultural and animal husbandry production, and in particular relates to an in-ovo injection for improving the anti-Salmonella ability of newborn poultry and its application. Background technique [0002] Salmonella is a common highly pathogenic pathogenic microorganism that seriously endangers the health of poultry. It mainly infects young chickens that lack a mature immune system. After infection, the mortality of chickens less than 3 weeks old can reach 40%. Carry germs, form recessive infection, and then lead to residues in poultry meat and egg products. It is very difficult to detect poultry products with residual Salmonella, especially poultry egg products, Salmonella may not remain on the surface of the eggshell, and there will be no Salmonella residues in the egg white due to the presence of bacteria-inhibiting substances, so Salmonella is usually in the egg yolk exists, but the uncertainty of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/715A61K31/7048A61K36/481A61P31/04
CPCA61K31/715A61K36/481A61K9/0019A61P31/04A61K31/7048
Inventor 张弋沈莎莎程君向贤真廖乐婕廖泽群邹波
Owner PANZHIHUA UNIV
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