Intra-ovum injection for improving salmonella resistance of newborn poultry and application
A Salmonella and injection technology, applied in the field of intra-ovo injection, can solve the problems of slow onset of traditional Chinese medicine, residual antibiotics, adverse reactions, etc., to improve the ability to resist Salmonella, improve the speed of maturation, and no drug residues.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0053] The present invention also provides a preparation method of the injection, which includes the following steps: heating and dissolving the above-mentioned active ingredient in the above-mentioned injection carrier under the condition of avoiding light, and then storing it in the dark after being sterilized.
[0054] Further, the present invention also provides the preparation method of the injection, comprising the following steps: adding astragalus polysaccharide and / or its pharmaceutically acceptable salt and / or ester at a concentration of 0.5-10% at 65°C in a dark environment Heat and dissolve in sterile DMEM cell culture fluid, or a pharmaceutically acceptable diluent for embryo or cell injection including glucose solution and / or PBS buffer, and then sterilized and stored away from light.
[0055] The present invention also provides a storage method for the injection, which includes the following steps: the injection is stored at -20°C for no more than 3 months or at 4°...
Embodiment 1
[0065] Embodiment 1 (preparation of injection of the present invention)
[0066] Solarbio’s 1g of astragalus polysaccharide dry powder is dissolved in 100g of sterile DMEM cell culture liquid at a concentration of 1%, heated and dissolved in sterile PBS buffer at 65°C, and then stored in the dark after sterilization.
[0067] Sterile DMEM cell culture fluid, glucose solution, and PBS buffer have the same function and effect in injection, and can be replaced with each other.
Embodiment 2
[0068] Embodiment 2 (the usage method of injection of the present invention)
[0069] Take SPF eggs or chicken embryos that have been hatched to 16 days old, and determine the position of the yolk sac at the small head of the chicken embryo according to the egg; after disinfecting the surface of the eggshell with 75% alcohol, drill a hole at the position of the yolk sac with a 1mm diameter micro electric drill; take out the egg from the refrigerator Invented injection, preheated at 37.5°C for 5 minutes before injection; took a 1mL syringe with a diameter of 0.7mL to draw the injection, and inserted it into the yolk sac of a chicken embryo through a hole at the small end, with an injection depth of 15mm and an injection dose of 0.5mL; Seal; each egg injection operation time does not exceed 5 minutes; in order to avoid cross-infection between eggs, each egg uses a separate syringe.
PUM
Property | Measurement | Unit |
---|---|---|
diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com