Composition for improving sleep and memory as well as preparation method and application of composition
A technology for improving sleep and composition, which is applied in the field of composition for improving sleep and memory and its preparation, which can solve problems such as ineffective sleep-aiding effect, and achieve the effect of no adverse reaction, definite effect, and quick effect
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Embodiment 1
[0065] The typical preparation method of the extract of embodiment 1 composition of the present invention
[0066] Get gardenia 167g (batch number: 2012021-W, place of origin: Fujian, supplier: Bozhou City, Anhui Province Chinese and Western Medicine Co., Ltd.), dried ginger 100g (lot number: 1910008-W, place of origin: Shandong, supplier: Bozhou City, Anhui Province) Western Medicine Co., Ltd.), licorice 100g (batch number: 2012011-W, place of origin: Inner Mongolia, supplier: Anhui Runbang Chinese Herbal Pieces Co., Ltd.), properly crushed, added 6 times the amount of water, steamed at 100°C for 1 hour, filtered to obtain extraction solution, repeat the above cooking 3 times, combine the extracts, filter and concentrate to obtain the extract, and mix the extract obtained above with 310g of deer glue evenly while it is hot, and dry it in an oven at 70°C for 2 days to obtain 405g of Chinese medicine composition powder N.
Embodiment 2
[0067] Embodiment 2 Zebrafish improves sleep efficacy evaluation
[0068] 2.1 Experimental animals:
[0069] The wild-type AB strain zebrafish (provided by Hangzhou Huante Biology Co., Ltd.) was reproduced by natural pair mating, with a total of 180 fish, 30 fish in each experimental group, and the age was 5 days after fertilization (5dpf). All zebrafish were raised in fish culture water at 28°C (water quality: 200 mg of instant sea salt was added to 1 L of reverse osmosis water, the conductivity was 450-550 μS / cm; the pH was 6.5-8.5; the hardness was 50-100 mg / L CaCO 3 ), the license number for the use of experimental animals is: SYXK (Zhejiang) 2012-0171. The feeding management complies with the requirements of the international AAALAC certification.
[0070] 2.2 Experimental method:
[0071] 1) Determination of the maximum detection concentration (MTC): randomly select 180 tails of 5dpf wild-type AB strain zebrafish in a six-hole plate, process 30 tails in each well, and...
Embodiment 3
[0086] Example 3 Preliminary study on the mechanism of zebrafish improving sleep
[0087] 3.1 Experimental animals:
[0088] The wild-type AB strain zebrafish (provided by Hangzhou Huante Biology Co., Ltd.) was reproduced by natural pair mating, with a total of 360 fish, 30 fish in each experimental group, and the age was 5dpf.
[0089] 3.2 Experimental method:
[0090] Randomly select 360 5dpf wild-type AB strain zebrafish in a six-well plate, and treat 30 tails in each well, and give Chinese medicine composition powder N500, 1000 μg / mL concentration in water solution, oxazepam 29 μg / mL concentration in the positive control group, and at the same time Set the normal control group and the model control group, and the volume of each well is 3mL. After incubation in a 28°C incubator for 24 hours, except the normal control group, the rest of the experimental groups were induced with 691 μg / mL PTZ to establish the insomnia model, and three parallel experiments were set up. Afte...
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