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Tissue engineering bone gel and application thereof

A tissue-engineered bone and gel technology, applied in tissue regeneration, medical science, prostheses, etc., can solve the problems of abnormal bone tissue hyperplasia, difficult to play a supporting role, low biomechanical strength of gel, etc., to stimulate cells Osteogenic differentiation, reduce the risk of infection, good support effect

Pending Publication Date: 2021-11-02
红色未来科技(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the preparation method of the decellularized material used in the current patent contains strong immunogenicity, and the biomechanical strength of the gel is low, so it is difficult to play a supporting role, resulting in abnormal proliferation of bone tissue. Therefore, a new tissue-engineered bone is needed. Gel to solve the above problems

Method used

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  • Tissue engineering bone gel and application thereof
  • Tissue engineering bone gel and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The tissue engineering bone gel of this embodiment is composed of bone decellularized material, physiological saline and cross-linked dextran, and the dosage ratio of bone decellularized material, physiological saline and cross-linked dextran is 0.1:1:1.

[0031] Wherein, the preparation method of bone decellularized material is as follows:

[0032] (1) Take SPF grade mouse bone tissue, cut into small pieces of about 3 mm, rinse with sterile PBS for 3 times, each time for 30 minutes, to remove blood and other impurities;

[0033] (2) In 7% PBS buffer solution containing 10 KIU / ml protease inhibitor, shake at 100 rpm for 3 hours at a constant temperature of 37°C; wash with PBS for 6 times;

[0034] (3) Add 10KIU / ml, 10KIU / ml penicillin-streptomycin solution to the PBS buffer solution containing DNase at a concentration of 0.2mg / ml, and the volume ratio of the buffer solution to the penicillin-streptomycin solution is 8:1 , shake at 100rpm at 37°C for 10h, wash with PBS ...

Embodiment 2

[0039] The tissue engineering bone gel of this embodiment is composed of bone decellularized material, physiological saline and cross-linked dextran, and the dosage ratio of bone decellularized material, physiological saline and cross-linked dextran is 0.5:1:1.

[0040]Wherein, the preparation method of bone decellularized material is as follows:

[0041] (1) Take SPF grade mouse bone tissue, cut into small pieces of about 3 mm, rinse with sterile PBS for 5 times, each time for 10 minutes, to remove blood and other impurities;

[0042] (2) In 8% PBS buffer solution containing 10 KIU / ml protease inhibitor, shake at 150 rpm for 2 hours at a constant temperature of 37°C; wash with PBS 8 times;

[0043] (3) Add 10KIU / ml, 10KIU / ml penicillin-streptomycin solution to 0.4mg / ml DNase-containing PBS buffer, and the volume ratio of buffer and penicillin-streptomycin solution is 5:1 , shake at 150rpm on a mg shaker at 37°C for 8h, wash with PBS 8 times;

[0044] (4) Add 10 KIU / ml and 1...

Embodiment 3

[0048] The tissue engineering bone gel of this embodiment is composed of bone decellularized material, physiological saline and cross-linked dextran, and the dosage ratio of bone decellularized material, physiological saline and cross-linked dextran is 1:1:1.

[0049] Wherein, the preparation method of bone decellularized material is as follows:

[0050] (1) Take SPF grade mouse bone tissue, cut into small pieces of about 3 mm, rinse with sterile PBS 4 times, each time for 20 minutes, to remove blood and other impurities;

[0051] (2) In 7% PBS buffer solution containing 10KIU / ml protease inhibitor, shake at 120rpm for 2 hours at a constant temperature of 37°C; wash with PBS for 7 times;

[0052] (3) Add 10KIU / ml, 10KIU / ml penicillin-streptomycin solution to 0.3mg / ml DNase-containing PBS buffer solution, the volume ratio of buffer solution and penicillin-streptomycin solution is 6:1, Shake at 120rpm at 37°C for 9h, wash with PBS 7 times;

[0053] (4) Add 10 KIU / ml, 10 KIU / ml...

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Abstract

The invention belongs to the technical field of tissue engineering, and particularly relates to a tissue engineering bone gel and the application thereof. The tissue engineering bone gel comprises a bone decellularized material, cross-linked glucan and normal saline, and the volume ratio of the bone decellularized material to the cross-linked glucan to the normal saline is (0.1-1): 1: 1. The bone gel can be used as a bone repair material for bone defect parts. The invention has the beneficial effects that the cross-linked dextran is combined with a bone acellular material, so that the osteogenic differentiation of cells is promoted to a great extent, and the method has relatively good supporting property and low immunogenicity.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering, in particular to a tissue engineering bone gel and its application. Background technique [0002] At present, the clinical treatment of critical bone defects caused by trauma, infection, tumor or congenital diseases still tends to be based on autologous or allogeneic bone transplantation. However, issues such as severe donor site morbidity, high risk of infection, and transportation hinder their development as sustainable bone repair materials. [0003] Tissue engineering technology based on biodegradable porous scaffolds is considered to be a feasible and effective alternative strategy for bone regeneration repair. Nowadays, the materials that have been widely used in bone tissue engineering scaffolds are mostly synthetic degradable aliphatic polyesters, such as polylactic acid, poly(lactic acid-glycolic acid), polycaprolactone, etc. This synthetic material often lacks bioactive com...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/20A61L27/52A61L27/56A61L27/58
CPCA61L27/365A61L27/3687A61L27/20A61L27/58A61L27/56A61L27/52A61L2430/02C08L5/02
Inventor 李长明
Owner 红色未来科技(北京)有限公司
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