Detection kit of paclitaxel metabolism marker and detection method and application thereof

Abstract

The invention discloses a detection kit of paclitaxel metabolism markers as well as a detection method and application of the detection kit. The detection kit is used for detecting gene polymorphism of two genes CYP1B1 432C / G and ABCB1C3435T of the paclitaxel metabolism markers, and the kit comprises the following components: a CYP1B1 432C / G amplification primer, a CYP1B1 432C / G sequencing primer, an ABCB1C3435T amplification primer, an ABCB1C3435T sequencing primer and a positive control. According to the present invention, the multiple RPA amplification and the optimized pyrosequencing technology are adopted as the combination to detect the gene polymorphism related to the prediction of the treatment effect and the adverse reaction of the paclitaxel, and the kit can simultaneously detect the CYP1B1 (432C / G) and the ABCB1 (C3435T) gene polymorphism paclitaxel so as to provide the gene perspective suggestion for the clinical personalized medication.

Description

technical field [0001] The invention relates to a paclitaxel metabolic marker detection kit, a detection method and an application thereof, and belongs to the field of gene detection. Background technique [0002] Paclitaxel, a natural anticancer drug, as a diterpene alkaloid compound with anticancer activity, has been widely used clinically in the treatment of breast cancer, ovarian cancer, some head and neck cancers and lung cancer. Microtubules, a component of eukaryotic cells, are formed by dimers of microtubules composed of two similar polypeptide (a and p) subunits. Under normal conditions, there is a dynamic equilibrium between microtubules and tubulin dimers. Paclitaxel can lose this dynamic balance between the two, induce and promote tubulin polymerization, prevent depolymerization, and stabilize microtubules. Paclitaxel inhibits the normal dynamic regeneration of the microtubule network necessary for mitosis, prevents the formation of the normal mitotic spindle, ...

AI Technical Summary

Problems solved by technology

Among them, the sequencing method and the chip method have cumbersome operation steps, long detection cycle, and the amplification product is prone to contamination; the high-resolution melting curve method has simple steps, low specificity, and high requirements for equipment; The specific amplification method uses ARMS primers for specific amplification, and the design of the primers is difficult to optimize, and the detection conditions are strict.

Taqman fluorescent probe method has high test cost, and the amplification throughput of multiple genes is not high

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