Vascularized double-layer engineering skin based on 3D printing and preparation method thereof
A 3D printing and engineering skin technology, applied in the field of bioengineering, can solve problems such as insufficient skin sources, achieve precise control of the formation of blood vessel branches, achieve precise control, and promote the effect of cell proliferation
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0101]Vascularized double-layer engineered skin based on 3D printing. The vascularized double-layer engineered skin includes dermis skin tissue and epidermis skin tissue. The dermis skin tissue is obtained by 3D printing based on tension control; the epidermis skin tissue is inoculated on The surface of the dermis skin tissue.
[0102] For this vascularized double-layer engineered skin, the stretching force can be controlled by changing the distance between the 3D printed frame structures during the construction process, which can not only effectively promote the proliferation of cells in the skin tissue of the epidermis, but also precisely control the formation of blood vessel branches. Tissue-engineered skin with normal bilayer skin tissue provides effective support and expands the clinical application of tissue-engineered skin in wound repair.
Embodiment 2
[0104] Based on 3D printing, vascularized double-layer engineered skin includes dermis skin tissue and epidermis skin tissue. The dermis skin tissue is a complex of GelMA-fibrin-endothelial cells and fibroblasts controlled by 3D stretching force. Obtained by printing; the epidermis skin tissue is inoculated on the surface of the dermis skin tissue;
[0105] Endothelial cells include endothelial cells induced by adipose stem cells or human umbilical vein endothelial cells, preferably human umbilical vein endothelial cells; wherein, the fibrin concentration is 5-10 mg / ml, and can be selected as 5.5 mg / ml, 6 mg / ml, 6.5 mg / ml, 7mg / ml, 7.5mg / ml, 8mg / ml, 8.5mg / ml, 9mg / ml, 9.5mg / ml, preferably 7.5mg / ml;
[0106] The epidermal skin tissue is obtained by culturing epidermal stem cells or keratinocytes on the surface of the dermal skin tissue; preferably epidermal stem cells;
[0107] The epidermal stem cells are 1-3 generations of epidermal stem cells derived from skin tissue; prefera...
Embodiment 3
[0139] Preparation method of vascularized bilayer engineered skin based on 3D printing, such as figure 1 shown, including the following steps:
[0140] step one( figure 1 A): Establish a polycaprolactone PCL frame structure with a height of 400 μm, and the PCL in the dotted line is as follows figure 2 , image 3 The movable PCL structure shown, can apply tension;
[0141] Step two ( figure 1 B): Set grooves on the polycaprolactone PCL frame structure, the groove depth is 90 μm, and the width is 8mm; the spacing of the PCL frame is set to 5mm and 9mm respectively according to the requirements of blood vessel branches;
[0142] Step three ( figure 1 C): The bottom of the tissue is filled with gelatin hydrogel;
[0143] Step four ( figure 1 D): printing (filling) a layer of GelMA-fibrin-adipocyte stem cell-induced differentiation in the groove to form a complex of endothelial cells and fibroblasts;
[0144] Among them, the concentration of GelMa is 25 mg / ml, and the ...
PUM
Property | Measurement | Unit |
---|---|---|
concentration | aaaaa | aaaaa |
depth | aaaaa | aaaaa |
width | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com