ERA nucleic acid test strip amplification kit for rapidly detecting feline herpes virus, preparation method and detection method

Abstract

The invention discloses an ERA nucleic acid test strip amplification kit for rapidly detecting feline herpes virus, a preparation method and a detection method. The ERA nucleic acid test strip amplification kit can effectively amplify target genes. The sensitivity can detect 7.86 * 10<1> copies / [mu]L; the specificity is high, and cross reaction is avoided; and the repeatability is good, and the intra-group and inter-group variable coefficients are smaller than 5%. The ERA isothermal amplification system is rapid in reaction and wide in temperature range, target genes can be effectively amplified at 37-40 DEG C, but the amplification effect is optimal after the reaction is carried out at 37 DEG C for 30 minutes. The amplification kit can rapidly, efficiently and sensitively detect the feline herpes virus, has the characteristics of simplicity in operation, high specificity, safety, no pollution and the like, a reaction result is easy to observe, so that an effective technical means is provided for rapidly detecting and screening the feline herpes virus in situ, and the amplification kit has important significance on prevention and control of economic losses, caused by the FHV, of pets, tigers, leopards and other feline animal industries.

Description

technical field [0001] The invention designs an ERA nucleic acid test strip amplification kit for rapid detection of feline herpes virus, a preparation method and a detection method. Background technique [0002] Feline infectious rhinotracheitis (feline infectious rhinotracheitis) is caused by feline herpesvirus type 1 (feline herpesvirus-1, FHV-1), an acute and highly contagious infectious disease with more than respiratory symptoms. FHV-1 mainly infects cats and can cause clinical symptoms such as elevated body temperature, depression, anorexia, coughing, and sneezing. Clinically, the morbidity rate of kittens is 100%, and the mortality rate is as high as 50%. Although cats are the natural host of the virus, in recent years there have been frequent reports of FHV-1 infection in cats such as cheetahs and tigers. FHV-1 is one of the most important pathogens of upper respiratory tract infection and eye injury in cats. It is often co-infected with feline calicivirus, and ca...

AI Technical Summary

Problems solved by technology

FHV-1 is one of the most important pathogens of upper respiratory tract infection and eye injury in cats. It is often co-infected with feline calicivirus, and can also cause secondary infection of bacteria, which seriously threatens the health of feline animals. Prevention and control poses great difficulties

At present, the detection methods of FHV mainly include nucleic acid detection, virus isolation and identification, immunofluorescence detection and antibody detection, etc., all of which require expensive equipment and professional technicians to operate, and cannot meet the detection conditions of non-laboratory and other grass-roots field conditions Require

Images