Vaccine vector as well as preparation method and application thereof

A vaccine carrier and vaccine technology, which is applied in the field of biomedicine, can solve the problems of cumbersome and complicated processes and difficulty in large-scale production, and achieve the effect of significant tumor growth, simple and mild preparation conditions, and strong lymph node targeting

Active Publication Date: 2021-11-09
CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this kind of nanocapsule has certain flexibility, its preparation process needs to use silicon spheres as a template first, and then remove silicon oxide by dissolution method in the later stage. The process is cumbersome and complicated, and it is difficult to produce on a large scale.

Method used

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  • Vaccine vector as well as preparation method and application thereof
  • Vaccine vector as well as preparation method and application thereof
  • Vaccine vector as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Preparation of oxidized mannan

[0046] Dissolve 1 g of mannan in 10 mL of sterile water, stir until dissolved in an ice bath, dissolve 657 mg of sodium periodate in 5 mL of sterile water, and add dropwise to the mannan solution. After 2h, the ice bath was removed, and the reaction was carried out at room temperature and protected from light for 12h. The product was purified by dialysis and freeze-dried to obtain the final product.

[0047] The degree of oxidation of the final product was determined by the hydroxylamine hydrochloride method, and the specific operations were as follows: Take 0.1 mg of oxidized mannan, dissolve it in 25 mL of hydroxylamine hydrochloride aqueous solution (0.25mol / L, containing 0.05% methyl orange), and statically Set for 3h. Titrate with standard NaOH solution (0.1mol / L), the red color turns yellow, and the volume of NaOH consumed is recorded as V 1 . Another part of the same volume of hydroxylamine hydrochloride aqueous solution (0.25...

Embodiment 2

[0052] Preparation of oxidized mannan / PEI-CpG-OVA vaccine

[0053] Dissolve OVA protein in sterile water for injection to obtain an antigen solution with a concentration of 1mg / mL, dissolve PEI in sterile water for injection with a concentration of 1mg / mL, and dissolve CpG in sterile water for injection with a concentration of 1mg / mL, dissolve oxidized mannan in sterile water for injection, the concentration is 1mg / mL, add 1 volume of OVA solution to 4 volumes of PEI solution under vortex, vortex for 30s, and then add 1 volume of The CpG was added to the mixture, vortexed for 30s, and 2 volumes of oxidized mannan solution was added, vortexed for 30s, to obtain the oxidized mannan / PEI-CpG-OVA vaccine. The particle size was measured by a Malvern particle size analyzer and was 211 nm.

Embodiment 3

[0055] Preparation of PEI derivatives (PEI-4BImi)

[0056] 50 mg of PEI (Mw=10kDa) was dissolved in DMSO solution, 25 mg of benzimidazole-7-carboxylic acid, 60 mg of EDC·HCl, and 36 mg of NHS were dissolved in 10 mL of DMSO, stirred at room temperature, activated for 30 min, Added to the DMSO solution of PEI, reacted at room temperature for 72h. The product was purified by dialysis with sterile water for injection, and freeze-dried to obtain the final product PEI-4BImi.

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Abstract

The invention relates to the technical field of biological medicine, in particular to a vaccine vector and a preparation method and application thereof. The vaccine vector consists of oxidized mannan and a cationic polymer, the cationic polymer comprises at least one of polyethyleneimine, polyamide and poly beta urethane, or derivatives of the cationic polymer, or a block copolymer or graft copolymer of the cationic polymer and the derivatives thereof and polylactic acid or polylactic acid-glycolic acid. The vaccine vector disclosed by the invention is large in antigen loading capacity, simple and mild in vaccine preparation condition and good in stability, has excellent lymph node targeting and dendritic cell targeting capabilities, and can cause strong antigen-specific immune response.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a vaccine carrier, its preparation method and application. Background technique [0002] Tumor immunotherapy has made great progress, and tumor vaccines, as a potential immunotherapy, have received increasing attention. The advantages of tumor vaccines are strong specificity, safety and long-term effectiveness. Nano-tumor vaccines have gained more and more attention in recent years, because nano-tumor vaccines can: 1) load tumor antigens and adjuvants, prevent the degradation and premature diffusion of antigens and adjuvants; Immunostimulatory effect; 3) Utilizing the unique nanometer size advantage to efficiently return to lymph nodes and be captured by antigen-presenting cells. Nevertheless, the current development of nano-vaccine is not satisfactory, and its immunostimulatory effect and anti-tumor effect are limited, which limits its clinical promotion. [0003] The mai...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/36A61K47/34A61K39/00A61K9/51A61P35/00
CPCA61K47/36A61K47/34A61K39/0011A61K9/5161A61K9/5146A61K9/5192A61P35/00A61K2039/53
Inventor 宋万通徐玉笛赵佳雨刘丽萍汤朝晖陈学思
Owner CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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