An enteric coated oral gel vaccine for animals and preparation and using methods thereof

An animal, enteric-coated technology, applied in biochemical equipment and methods, veterinary vaccines, vaccines, etc., to achieve the effects of high antigen loading, low toxic and side effects, and scientific preparation methods

Inactive Publication Date: 2018-12-04
程金水
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to aim at the deficiencies and shortcomings of vaccines in the existing animal mucosal immunization process, to provide a kind of enteric-coated oral gel vaccine for animals and its preparation and use method, and to introduce gel technology to solve the problem of animal mucosal immunization vaccines. Problems in production practice

Method used

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  • An enteric coated oral gel vaccine for animals and preparation and using methods thereof
  • An enteric coated oral gel vaccine for animals and preparation and using methods thereof
  • An enteric coated oral gel vaccine for animals and preparation and using methods thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Embodiment 1: Porcine epidemic diarrhea virus enteric-coated oral gel vaccine and preparation method thereof

[0031] (1) Preparation of antigen particles: porcine epidemic diarrhea virus antigen 0.13%, sodium glycinate 1.5%, CpG-ODN 0.5%, microcrystalline cellulose-ethanol solution 10%, in a 100-level clean area, the porcine epidemic diarrhea The virus liquid was added to the microcrystalline cellulose-ethanol solution, then mixed evenly with sodium glycinate and CpG-ODN, dried at 40°C, and passed through a 20-mesh sieve to prepare antigen particles.

[0032] (2) Preparation of gel solution: Carrageenan 15%, sodium carboxymethylcellulose 10%, Tween-80 5%, sodium glutamate 0.5%, maltodextrin 69.5%, after mixing evenly according to the prescription amount, Under the condition of 30°C, prepare a 5% aqueous solution, that is, a gel solution.

[0033] (3) Preparation of porcine epidemic diarrhea virus enteric-coated oral gel vaccine: add antigen particles to the gel soluti...

Embodiment 2

[0034] Example 2: Chicken Campylobacter jejuni enteric-coated oral gel vaccine and its preparation method

[0035] (1) Preparation of antigen granules: 0.13% of Campylobacter jejuni antigen, 1.5% of trehalose, 0.5% of saponin, and 10% of microcrystalline cellulose ethanol solution. in ethanol solution, mixed with trehalose and saponin evenly, dried at 40°C, and passed through a 24-mesh sieve to prepare antigen particles.

[0036] (2) Preparation of gel solution: gelatin 15%, hydroxypropyl methylcellulose 11%, betaine 3%, cyclamate 0.3%, galactooligosaccharide 70.7%, after mixing evenly according to the prescription quantity, be mixed with 10.5 % of the aqueous solution is the gel solution.

[0037] (3) Preparation of chicken Campylobacter jejuni enteric-coated oral gel vaccine: add antigen particles to the gel solution and disperse evenly in a 10,000-class clean area, then cool to obtain.

Embodiment 3

[0038] Example 3: Piglet proliferative enteritis enteric-coated oral gel vaccine and its preparation method

[0039] (1) Preparation of antigen particles: porcine proliferative enteritis antigen 0.2%, sodium glycinate 0.5%, trehalose 0.5%, skimmed milk powder 0.5%, saponin 0.5%, microcrystalline cellulose ethanol solution 10%, in a class 100 clean area , the antigen solution is added to the microcrystalline cellulose ethanol solution, and then mixed evenly with sodium glycinate, trehalose, skim milk powder and saponin, dried at 25° C., and passed through a 30-mesh sieve to prepare antigen particles.

[0040](2) Preparation of gel solution: xanthan gum 15%, sodium carboxymethylcellulose 10%, Tween-20 5%, sodium glutamate 0.5%, lactose 69.5%, after mixing evenly according to the prescription amount, prepare into a 5.5% aqueous solution, which is the gel solution.

[0041] (3) Preparation of enteric-coated oral gel vaccine for piglet proliferative enteritis: Add antigen particle...

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Abstract

An enteric coated oral gel vaccine for animals and preparation and using methods thereof are disclosed. The preparation method includes preparing an antigen particle intermediate product from an animal pathogenic microorganism antigen, an antigen protecting agent, an adjuvant, and the like through a certain process; and then preparing the gel vaccine from the antigen particle intermediate productand a gel solution having a certain concentration. The prepared gel vaccine is enteric-coated, has a sustained release effect, is mainly released in small intestines, and can be used for animal vaccine immunization in manners of mixed drinking, mixed feeding, or direct feeding. The preparation method is scientific, simple and reasonable, production is stable and reliable, and a product yield is 95% or above. The gel technology is adopted, and a finished product has the advantages of a high antigen content, good uniformity, long sustained release time, few toxic and side effects, a low cost, convenient use, long storage life, and the like.

Description

technical field [0001] The invention relates to the technical field of biological new medicines, in particular to an enteric gel vaccine for animals and its preparation and use methods. Background technique [0002] At present, livestock, poultry, aquatic animal diseases and even wild animal vaccines are becoming more and more complicated. Most of them are mucosal diseases. Sexual and parasitic diseases are immunized with vaccines, which gradually reduces the occurrence of large-scale severe infectious diseases, ensures the healthy and sustainable development of breeding production, and ensures the steady improvement of people's living standards. However, in the process of using animal vaccines, there are also some shortcomings and shortcomings. Most of the current vaccine immunization methods are mainly injections. Some vaccines can be taken orally, sprayed, eye drops and nasal drops, etc., but there are not many types. In addition, in There are very few researches on vacc...

Claims

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Application Information

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IPC IPC(8): A61K39/215A61K9/06A61P31/14A61K39/012A61P33/02A61K39/02A61P31/04
CPCA61K9/0053A61K9/06A61K39/012A61K39/0208A61K39/12A61K47/38A61K47/42A61K2039/542A61K2039/55A61K2039/552A61K2039/572A61K2039/575A61P31/04A61P31/14A61P33/02C12N2770/20034
Inventor 郑仙忠程金水于博
Owner 程金水
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