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Application of potato StABL1 gene in regulation and control of potato maturity

A potato and gene technology is applied in the field of molecular genetics and breeding to achieve the effect of promoting tuber formation

Active Publication Date: 2021-11-12
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no relevant literature report on the function of StABL1 gene in potato tuber development

Method used

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  • Application of potato StABL1 gene in regulation and control of potato maturity
  • Application of potato StABL1 gene in regulation and control of potato maturity
  • Application of potato StABL1 gene in regulation and control of potato maturity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] This example provides a method for obtaining the transcription factor StABL1 gene associated with potato tuber development.

[0029] Class A bZIP family members in potato have functional redundancy and have SAP motifs that can interact with StSP6A (Teo et al., 2017), screened based on protein interactions (see figure 1 ), we found that StABI5like1, a member of the AREB / ABF / ABI5 subfamily, can interact with StSP6A, and its expression level is high in various developmental organs. Therefore, it is speculated that this gene may also be involved in the growth and development of potato, and we named the gene StABL1.

Embodiment 2

[0031] This embodiment provides the potato StABL1 gene, a cloning method and a function verification method, which specifically include the following steps:

[0032] (1) Primer design

[0033] According to the sequence (Soltu.DM.10G025990.1) (Soltu.DM.10G025990.1) (such as sequence table SEQ No.2) of the StABL1 gene published in the potato genome database (http: / / spuddb.uga.edu / ) in the present invention, design primers to amplify the gene The full-length CDS sequence, the primer sequence is as follows:

[0034] StABL1 F (5'-ATGGGATCTCAGGGTGGTG-3')

[0035] StABL1 R (5'-TTAGACGGGCGCGGAGCTTGTTCTG-3')

[0036] In addition, quantitative primers for StABL1 were designed, and the primer sequences are as follows:

[0037] qStABL1F(5'-GAACTGGAGAACAAGGTTTCAC-3')

[0038] qStABL1R(5'-GACATAACGAACACTACGCATC-3')

[0039] (2) RNA extraction

[0040] The leaf samples of the cultivar Epotas 3 (E3) were taken, ground into powder in liquid nitrogen, and RNA was extracted according to the ...

Embodiment 3

[0084] This embodiment provides the application of potato StABL1 gene, specifically as follows:

[0085] Using E3 as the genetic material, the transformed excess strains were OE-StABL1 (10, 13); using E109 as the genetic material, the transformed excess strains were OE-StABL1 (1, 2). In the tuber experiment in vitro, we transferred the single-segment excess lines OE-StABL1 (10, 13) and E3 into MS solid medium containing 8% sucrose, supplemented with 0.2% activated carbon, and placed in a short Sunlight growth chamber culture. From the fourth week, the tuber formation was recorded every week. In the in vivo tuber experiment, the excess strain OE-StABL1 (10, 13), the control E3, the excess strain OE-StABL1 (1, 2) and the control E109 were planted in the net room, and cultivated in long-day light for about 4 weeks (10-leaf stage), the excess strain OE-StABL1 (10, 13) and the control E3 were transferred to short-day light, and the phenotype was observed every 5 days. The excess ...

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Abstract

The invention provides application of a potato (solanum tuberosum) StABL1 gene in regulation and control of potato maturity. The StABL1 gene is cloned through a sequence published in a potato genome database by taking cDNA of a cultivated species E potato No.3 leaf as a template, an over-expression vector is constructed, and genetically transformed E potato No.3 and E109 are subjected to transgenic function verification; and it is found that overexpression of the StABL1 gene can promote prematurity of potatoes, and the overexpression of the StABL1 gene is mainly characterized by premature senescence of leaves, premature flowering, premature tuberization of underground parts, life cycle shortening and the like.

Description

technical field [0001] The invention belongs to the field of molecular genetic breeding, and in particular relates to the application of potato StABL1 gene in regulating potato maturity. Background technique [0002] Potato (Solanum tuberosum L.) is the fourth largest food crop in the world after rice, wheat and corn, and plays a vital role in food security. Its main edible parts are underground storage organs or tubers, which accumulate large amounts of starch and vitamin C. In potato production practice, ripeness and tuber starch content are two important agronomic traits in breeding. The content of starch affects the fresh taste and processing quality of potatoes, accounting for about 80% of the dry matter of tubers. Varieties with different ripeness can meet the needs of the market according to changes in different regions and climates. The potato tuber is used as a model to study the formation of specialized vegetative organs, characterized by a dual reproductive sys...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/82A01H5/02A01H5/00A01H6/82
CPCC07K14/415C12N15/8266C12N15/827C12N15/8261
Inventor 宋波涛景晟林孙小梦余柳王恩爽覃骏董烈鹏
Owner HUAZHONG AGRI UNIV
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