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Method for detecting aristolochic acid in soil

A detection method, aristolochic acid technology, applied in the field of detection of aristolochic acid in soil, to achieve the effects of improving detection accuracy, improving purification efficiency, and improving detection efficiency

Pending Publication Date: 2021-11-12
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For the detection of trace or even ultra-trace aristolochic acid, there are few reports at present

Method used

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  • Method for detecting aristolochic acid in soil
  • Method for detecting aristolochic acid in soil
  • Method for detecting aristolochic acid in soil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] The present invention provides a method of detecting the content of traces of traces in the soil, and the specific measurement steps are as follows:

[0066] (1) Pre-sample treatment

[0067] Crushing: After the soil sample is collected, the air dried debris, crusted 250 μm sieve, fully mixed, and booked in the sample bottle.

[0068] Extraction: Soil sample 5.0g in 50 ml of plastic centrifuge tubes, add 10.0 ml of pure water, mix well, add 10 ml of 1% acetonitrile, oscillated for 30 min;

[0069] Dehydration: Add 6.0 g of anhydrous anhydrous sulfate and 1.0 g sodium chloride, strenuous oscillate for 1 minute, centrifugation at 4 ° C for 3 minutes (8000 r / min).

[0070] Purification: Driven by 2.0 mL of extract, adding 0.3 g of magnesium sulfate, 0.1 gC18 5 ml of plastic centrifuge tubes, vortex oscillation for 1 min, 4 ° C centrifugation for 3 minutes (8000 r / min).

[0071] Filtering: The supernatant is 0.2 μm filtration, placed in a glass inlet.

[0072] (2) LC-MS / M...

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Abstract

The invention relates to the technical field of analytical chemistry and environmental detection, in particular to a method for detecting aristolochic acid in soil, and in particular relates to a high-sensitivity detection method for detecting aristolochic acid in soil through dispersive solid-phase extraction-liquid chromatography-tandem mass spectrometry. The invention provides the method for detecting aristolochic acid in soil. According to the method, a dispersive solid-phase extraction-liquid chromatography-tandem mass spectrometry technology is adopted; wherein an extraction solvent for dispersive solid-phase extraction is 0.1-2% acetic acid acetonitrile. According to the method, acetic acid acetonitrile is selected as an extraction solvent and has specific selectivity on aristolochic acid, so that the extraction rate of aristolochic acid can be increased to the greatest extent, more impurities in soil can be prevented from entering an extracting solution, and the accuracy of subsequent detection is improved; and the extraction solvent also has the advantages of high instrument response value, high sensitivity and high extraction efficiency.

Description

Technical field [0001] The present invention relates to analytical chemistry and environmental detection technologies, and particularly relates to a method for detecting soil aristolochic acid, particularly to a dispersive SPE - Determination of soil aristolochic acid with liquid chromatography tandem mass spectrometry of high sensitivity of the detection method. Background technique [0002] Aristolochic acid (Aristolochic acids, AAs) a nitro phenanthrene compounds, mainly containing aristolochic acid I (AA-I) and aristolochic acid II (AA-II) of the two components. Aristolochic acid widely found in Aristolochia Aristolochia Aristolochia Asarum Asarum and other plants having renal toxicity, carcinogenicity, and mutations induced by genotoxic. [0003] The current Chinese medicine containing aristolochic acid (such as Aristolochia, tetrandra, green wood, angel vine, etc.) are still growing, their stems and leaves, fruits, root and so scattered, left the soil, the soil is bound to ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/72G01N30/06G01N30/14G01N30/34G01N30/86G01N30/04
CPCG01N30/02G01N30/72G01N30/06G01N30/14G01N30/34G01N30/8634G01N30/04G01N2030/062G01N2030/146G01N2030/047Y02A50/30
Inventor 平华李成马智宏王北洪杜远芳孔红玲付海龙
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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