Method for detecting aristolochic acid in soil
A detection method, aristolochic acid technology, applied in the field of detection of aristolochic acid in soil, to achieve the effects of improving detection accuracy, improving purification efficiency, and improving detection efficiency
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[0064] Example 1
[0065] The present invention provides a method of detecting the content of traces of traces in the soil, and the specific measurement steps are as follows:
[0066] (1) Pre-sample treatment
[0067] Crushing: After the soil sample is collected, the air dried debris, crusted 250 μm sieve, fully mixed, and booked in the sample bottle.
[0068] Extraction: Soil sample 5.0g in 50 ml of plastic centrifuge tubes, add 10.0 ml of pure water, mix well, add 10 ml of 1% acetonitrile, oscillated for 30 min;
[0069] Dehydration: Add 6.0 g of anhydrous anhydrous sulfate and 1.0 g sodium chloride, strenuous oscillate for 1 minute, centrifugation at 4 ° C for 3 minutes (8000 r / min).
[0070] Purification: Driven by 2.0 mL of extract, adding 0.3 g of magnesium sulfate, 0.1 gC18 5 ml of plastic centrifuge tubes, vortex oscillation for 1 min, 4 ° C centrifugation for 3 minutes (8000 r / min).
[0071] Filtering: The supernatant is 0.2 μm filtration, placed in a glass inlet.
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