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Preparation method of MOFs coated cardiac muscle cell core-shell structure

A cardiomyocyte and nucleus technology, applied in biochemical equipment and methods, animal cells, microsphere preparation, etc., can solve the problems of bio-hybrid micro-robots such as lack of physical protection, nutrient supply channels, and short life cycle, so as to achieve life support , the effect of prolonging the service life

Inactive Publication Date: 2021-11-16
SHENZHEN POLYTECHNIC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to solve the problems of lack of physical protection, nutrient supply channels and short life cycle of existing bio-hybrid microrobots, and provide a method for preparing MOFs-coated cardiomyocyte core-shell structure

Method used

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  • Preparation method of MOFs coated cardiac muscle cell core-shell structure
  • Preparation method of MOFs coated cardiac muscle cell core-shell structure

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specific Embodiment approach 1

[0023] Embodiment 1: In this embodiment, a method for preparing a MOFs-coated cardiomyocyte core-shell structure is completed according to the following steps:

[0024] 1. Discard the supernatant of the HL-1 mouse cardiomyocyte culture medium, and wash the HL-1 mouse cardiomyocytes with PBS buffer or saline;

[0025] 2. Add the cleaned HL-1 mouse cardiomyocytes into cell culture flasks or cell culture dishes, and then add 0.25% trypsin PBS solution, so that the 0.25% trypsin PBS solution covers the HL-1 mouse cardiomyocytes;

[0026] 3. Put the cell culture flask or cell culture dish in step 2 into the CO with a temperature of 37°C. 2 Digest in an incubator, then add culture medium to stop digestion, and obtain cell suspension;

[0027] 4. Centrifuge the cell suspension, discard the supernatant, and obtain the cell pellet;

[0028] 5. Use fresh medium to resuspend the cell pellet, then add it to the cell culture bottle or cell culture dish, put the cell culture bottle or ce...

specific Embodiment approach 2

[0035] Embodiment 2: This embodiment differs from Embodiment 1 in that: the number of cleanings described in step 1 is 1 to 2 times. Other steps are the same as in the first embodiment.

specific Embodiment approach 3

[0036] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that the CO described in step 3 2 CO in the incubator 2 The volume fraction of the medium is 5%; the medium in step 3 consists of 90% DMEM high glucose, 9% fetal bovine serum and 1% double antibody; the concentration of the double antibody is 100 U / mL. Other steps are the same as those in Embodiment 1 or 2.

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Abstract

The invention discloses a preparation method of an MOFs coated cardiac muscle cell core-shell structure, and relates to a method for coating cardiac muscle cells. The invention aims to solve the problems that an existing biological mixing micro-robot lacks physical protection and nutrient supply channels and is short in life cycle. The method comprises the following steps: 1, cleaning the positions; 2, adding a pancreatin PBS solution; 3, digesting the structure; 4, centrifuging the mixture; 5, culturing the tissue; 6, performing passage culture; 7, preparing a Zn(NO3)2.6H2O aqueous solution and a 2-methylimidazole aqueous solution; 8, adding the Zn(NO3)2.6H2O aqueous solution and the 2-methylimidazole aqueous solution for culture so as to obtain the MOFs coated cardiac muscle cell core-shell structure, wherein after 7 days of culture under the same culture condition, the cell density of the cardiac muscle cell with the MOFs shell layer is obviously higher than that of the cardiac muscle cell. According to the invention, the MOFs coated cardiac muscle cell core-shell structure can be obtained.

Description

technical field [0001] The invention relates to a method for coating cardiomyocytes. Background technique [0002] Cardiomyocytes can generate spontaneous, rhythmic contraction without any external assistance, so it is relatively easy to use cardiomyocytes to fabricate biohybrid microrobots. Given that the size of a single cardiomyocyte is about 100 μm and its ability to contract autonomously, a single cardiomyocyte can be used as a driver for a microrobot on the scale of hundreds of microns. This will provide new ideas for solving the energy supply and nutrition supply problems of micro-robots in special working environments (such as human body). [0003] However, living bio-actuated materials in biohybrid microrobots often have short life cycles, poor environmental robustness, and lack of proper coupling mechanisms with artificial micro-nanostructures, which have been the bottleneck problems that limit the biomedical applications of biohybrid microrobots. . This is main...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/077B01J13/02B01J13/20
CPCC12N5/0657B01J13/02B01J13/20C12N2533/30B01J13/04C12N5/0012C12N2500/22
Inventor 李晓琳陈伟张亮龚涛
Owner SHENZHEN POLYTECHNIC
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