Sample diluent, preparation method thereof and immunoassay method for eliminating detection abnormality of fresh sample

A diluent and sample technology, applied in the direction of analysis materials, measuring devices, biological testing, etc., can solve problems such as abnormal test results, reduce non-specific reactions, improve accuracy and precision, and ensure authenticity and reliability Effect

CN113671170APending Publication Date: 2021-11-19AUTOBIO DIAGNOSTICS CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
Publication Date
2021-11-19

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Abstract

The invention relates to the technical field of in-vitro diagnosis, in particular to a sample diluent, a preparation method thereof and an immunoassay method for eliminating abnormal detection of a fresh sample. The sample diluent comprises a component A, a component B and a biological buffer solution; the component A is lipoic acid or dihydrolipoic acid; and the component B is dextran sulfate or a salt thereof. According to the invention, the lipoic acid and the dextran sulfate are combined and applied to immune clinical detection, so that the abnormality of detection results caused by different freshness degrees of samples is avoided. The diluent can effectively prevent interference in a fresh sample, and can eliminate complement, fibrin and other main interfering substances contained in the sample. The diluent can reduce non-specific reaction in immunoassay, so that the accuracy and precision of a reagent are improved. The diluent is simple in preparation method, and has safety and environmental friendliness.
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Description

technical field

[0001] The invention relates to the technical field of in vitro diagnosis, in particular to a sample diluent, a preparation method thereof and an immunoassay method for eliminating abnormal detection of fresh samples. Background technique

[0002] The common interference in the immune response includes endogenous and exogenous interference. Common endogenous interference factors include complement, rheumatoid factor RF, autoantibodies, heterophilic antibodies, etc.; exogenous interference factors mainly include lipemia, bacterial Infection, hemolysis, high bilirubin, fibrin, etc.

[0003] Complement is a group of enzymatically active proteins present in human or vertebrate serum and interstitial fluid. Because it is a necessary supplement for antibodies to exert cytolytic effect, it is called complement. The intrinsic components of the complement system are produced by macrophages, liver cells, intestinal epithelial cells, spleen cells, etc. Activation of ...

Examples

Embodiment 1

[0031] 1. Diluent formula and preparation method

[0032] Firstly, the diluent of the present invention is prepared, which contains 0.05% lipoic acid, 0.1% dextran sulfate and PBS buffer in mass ratio. Preparation method: First, fully dissolve lipoic acid and dextran sulfate in the above ratio in 0.1mol / L PBS solution, mix well, and then adjust the pH to 7.5 for experimental research.

[0033] The control dilution 1 contains only 0.05% lipoic acid and PBS buffer; the control dilution 2 contains only 0.1% dextran sulfate and PBS buffer. The preparation method of control diluent 1 and control diluent 2 is the same as above.

[0034] 2. Detection method

[0035] The method for detecting the content of 25-hydroxyvitamin D in human serum or plasma samples using the diluent prepared by the present invention specifically comprises the following steps:

[0036] 1. Prepare magnetic particle suspension coated with anti-25 hydroxyvitamin D antibody;

[0037] 2. Preparation of gradien...

Embodiment 2

[0051] 1. Diluent formula and preparation method

[0052] Firstly, the buffer solution of the present invention is prepared, which contains 0.5% lipoic acid, 2.5% dextran sulfate and MOPS buffer solution in mass ratio. Preparation method: First, fully dissolve lipoic acid and dextran sulfate in the above ratio in 0.5 mol / L MOPS buffer solution, mix well, and then adjust the pH to 9.0 for experimental research.

[0053] The control dilution 1 contained only 0.5% lipoic acid and MOPS buffer; the control dilution 2 contained only 2.5% dextran sulfate and MOPS buffer. The preparation method of control diluent 1 and control diluent 2 is the same as above.

[0054] 2. Detection method

[0055] The method for detecting the content of trans-triiodothyronine in human serum or plasma samples using the diluent prepared by the present invention specifically comprises the following steps:

[0056] 1. Prepare magnetic particle suspension coated with anti-triiodothyronine antigen;

[005...

Embodiment 3

[0070] 1. Diluent formula and preparation method

[0071] Firstly, the buffer solution of the present invention is prepared, which contains 0.25% lipoic acid, 0.75% dextran sulfate and Tris-HCl buffer solution in mass ratio. Preparation method: First, fully dissolve lipoic acid and dextran sulfate in the above ratio in 0.1mol / L Tris-HCl buffer solution, mix well, and then adjust the pH to 8.5 for experimental research.

[0072] The control dilution 1 is a buffer containing only 0.25% lipoic acid and Tris-HCl; the control dilution 2 is a buffer containing only 0.75% dextran sulfate and Tris-HCl. The preparation method of control diluent 1 and control diluent 2 is the same as above.

[0073] 2. Detection method

[0074] The method for detecting the content of osteocalcin in human serum or plasma samples using the diluent prepared by the present invention specifically comprises the following steps:

[0075] 1. Preparation of magnetic particle suspension coated with anti-osteoc...