Peptide for reducing hair loss and promoting hair growth, and cosmetic composition and pharmaceutical composition comprising same

A cosmetic composition and hair growth technology, applied in cosmetics, cosmetic preparations, hair care, etc., can solve problems such as poor hair loss, erectile dysfunction, etc., to promote keratinocyte proliferation, regulate hair follicle growth, improve The effect of cell regeneration

Pending Publication Date: 2021-11-19
因科斯医药公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Minoxidil is a drug that induces hair growth by increasing blood flow through a vasodilator effect and supplying nutrients to the hair root, and is known to be effective in alleviating hair loss symptoms on the crown, but it must be taken for a long time and has effects on other than the crown. Partial hair loss effect is not very good disadvantage
Propecia, which is used for oral administration, also needs to be taken continuously and regularly. It is reported that when women take it for a long time, the probability of giving birth to deformed children is high. For some patients, it has side effects such as loss of libido and erectile dysfunction.

Method used

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  • Peptide for reducing hair loss and promoting hair growth, and cosmetic composition and pharmaceutical composition comprising same
  • Peptide for reducing hair loss and promoting hair growth, and cosmetic composition and pharmaceutical composition comprising same
  • Peptide for reducing hair loss and promoting hair growth, and cosmetic composition and pharmaceutical composition comprising same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0086] Example 1: Confirmation of the activation effect of the peptide on the Wnt / β-catenin signal transduction pathway

[0087] In order to analyze the activation of the Wnt / β-catenin signaling pathway in cells treated with the peptides of SEQ ID NO: 5 and SEQ ID NO: 10 shown in Table 1 below, β-catenin, glycogen synthesis Western blot of enzyme kinase 3β and p-glycogen synthase kinase 3β protein expression.

[0088] Human follicular dermal papilla cells (HFDPC) were mixed with 1×10 5 or 5×10 4 The number of cells was uniformly inoculated on a 6-well plate for culture, in Dulbecco's Modified Eagle Medium (DMEM, Dulbecco's Modified Eagle Media, Gibco BRL), at a temperature of 37°C, 5% CO 2 Conditions were incubated for 24 hours in the incubator. After that, multiple peptides of SEQ ID NO: 5 and SEQ ID NO: 10 were dissolved in water to a concentration of 10 mM to prepare a concentrated solution, which was diluted to a concentration of 200 uM, 100 uM, and 20 uM in a culture ...

Embodiment 2

[0092] Example 2: Finding the optimal concentration for activation of Wnt / β-catenin signal transduction pathway

[0093] The optimal concentration for activating the Wnt / β-catenin signal transduction pathway was found by treating the peptides of SEQ ID NO: 5 and SEQ ID NO: 10 according to the concentration of the present invention. The specific experimental method is as follows: in the case of SEQ ID NO:5, the concentration is diluted to 0.1 μM, 0.5 μM, 1 μM, 5 μM, 10 μM, 20 μM, 40 μM, 60 μM, 80 μM, 100 μM, in addition, the same as in Example 1 same in Figure 3a The result is shown. In the case of SEQ ID NO: 10, the concentration was diluted to 0.1 μM, 0.5 μM, 1 μM, 5 μM, 10 μM, 20 μM, 40 μM, 50 μM, except that it was the same as in Example 1, in Figure 3b The result is shown.

[0094] exist Figure 3a and Figure 3bIt can be seen that the peptides of SEQ ID NO: 5 and SEQ ID NO: 10 of the present invention were confirmed to increase the expression of active β-catenin pr...

Embodiment 3

[0095] Example 3: Confirmation of DKK-1 Activity Inhibition

[0096] The SEQ ID NO:5 and SEQ ID NO:10 peptides of the invention were processed to analyze whether the Wnt / β-catenin signaling pathway inhibited by DKK-1 was reactivated.

[0097] Specifically, human dermal papilla cells were divided into 5×10 4 The number of cells was uniformly seeded on a 12-well plate for culture, in Dulbecco's modified Eagle's medium, at a temperature of 37 ° C, 5% CO 2 Conditions were incubated for 24 hours in the incubator. After culturing, each well was treated with recombinant protein DKK-1 to inhibit the Wnt / β-catenin signal transduction pathway. At the same time, after additional treatment with the SEQ ID NO:5 and SEQ ID NO:10 peptides of the present invention at an appropriate concentration, Cultivate for a specified time.

[0098] After the culture was completed, the medium was removed, the cells were disrupted with sodium dodecyl sulfate sample buffer, and the proteins were separate...

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PUM

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Abstract

The present invention relates to a peptide for reducing hair loss or promoting hair growth, and a cosmetic composition and a pharmaceutical composition comprising the same, and specifically, a cosmetic composition, and a pharmaceutical composition for treatment may be provided, which are capable of reducing hair loss and promoting hair growth by activating a Wnt / [beta]-catenin signaling pathway, and at the same time, promoting the activity of the collagenase MMP-2 and ALP proteins and growth hormones needed for hair growth.

Description

technical field [0001] The present invention relates to a peptide for preventing or improving alopecia and a composition containing the same, and more specifically, to a peptide for relieving hair loss or promoting hair growth by activating the Wnt / β-catenin signal transduction pathway, and a cosmetic containing the same combination. Background technique [0002] Usually, the average person will lose about 100 hairs every day, and new hairs will grow out at the same time, so the number of scalp hairs is not easy to reduce. Hair loss refers to a state where there is no hair or less hair where there should be hair, and it is also classified as a progressive disease caused by the failure of the nutrient supply and metabolism required for hair growth. [0003] The factors that cause hair loss are not clear, but it can be due to various factors such as aging, genetic factors, stress, androgen action, blood circulation disorder, abnormal sebum secretion, demodex, malnutrition, im...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K5/08C07K7/06A61K8/64A61K38/06A61K38/08A61P17/14A61Q7/00
CPCC07K5/08C07K7/06A61K8/64A61K38/06A61K38/08A61P17/14A61Q7/00A61K2800/5922A61K2300/00A61K38/00C07K5/0817
Inventor 郑和志申嘉荣金兴载朴基燉
Owner 因科斯医药公司
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