Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Krachaidum total flavanone extract as well as preparation method and application thereof

A technology of total flavonoids and extracts, applied in the field of medicine, can solve the problems of toxicity risk of organic reagents, complex extraction reagents, harsh conditions of crushing steps, etc., and achieve the effects of improving bioavailability, high purity of extracts and prolonging lifespan

Active Publication Date: 2021-11-26
TSINGHUA UNIV
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the disadvantage of this method is that the conditions of the crushing step are relatively harsh, and the extraction reagents are complex, which may lead to potential toxicity risks of organic reagent residues

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Krachaidum total flavanone extract as well as preparation method and application thereof
  • Krachaidum total flavanone extract as well as preparation method and application thereof
  • Krachaidum total flavanone extract as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1: Optimization of the Extraction Method of Total Flavonoids of Kaempferia kaempferi

[0061] Instruments: LC-20AT High Performance Liquid Chromatography (Shimadzu Corporation); KQ-500DE CNC Ultrasonic Cleaner (Kunshan Ultrasonic Instrument Co., Ltd.); RT-12SF Pulverizer (Tianjin Test Instrument Co., Ltd.) ; MS104S analytical balance (Ningbo Xinzhi Biotechnology Co., Ltd.); 78100-11 acid-proof refrigerated desktop concentrator (Labconco, USA); LD5-2B centrifuge (Beijing Jingli Centrifuge Co., Ltd.), Milli-Q ultra Pure water instrument (Milipore, USA); Eppendorf centrifuge 5417R small high-speed refrigerated centrifuge (Germany Professional Life Science Company).

[0062] Liquid chromatography detection conditions: Lichrospher RP-18endcapped chromatographic column (4.6mm×250mm, 5μm); mobile phase: 0.5% formic acid aqueous solution (A) and 0.5% formic acid acetonitrile solution (B), gradient elution (0~10min, 38 %B; 10~20min, 38%~40%B; 20~35min, 40%B; 35~50min, 4...

Embodiment 2

[0073] Example 2: Comparative analysis of different ethanol concentrations on the extraction rate of total methoxylated flavonoids

[0074] The solid-to-liquid ratio was fixed at 1:12 (g / mL), the ultrasonic time was 25 min, and the ethanol concentrations were set at 30%, 60%, and 90%, respectively. Take an appropriate amount of extract to prepare samples for HPLC detection and analysis. The percentage content of medicinal materials of 6 kinds of methoxyflavones is plotted against the concentration of ethanol, see figure 2 . When the ethanol concentration is 60%, the extraction rate of the six methoxyflavones is the highest; when the ethanol concentration is 90%, the extraction rate of the six methoxyflavones is 5.44%, slightly lower than the extraction rate when the ethanol concentration is 60% ; When the ethanol concentration was 30%, the extraction rate of the six methoxyflavones was only 3.15%. When the ethanol concentration is low, the extraction yield of total methoxy...

Embodiment 3

[0075] Example 3: Purification and enrichment method of total flavonoids of Kaempferia kaempferi

[0076] Purification and enrichment of the total flavonoids of kaempferia by column chromatography. Firstly, three different types of macroporous resins AB-8, HPD300 and NKA-2 were selected to investigate their adsorption performance on flavonoids. Specific steps are as follows:

[0077] (1) Concentrate the kaempferia extract by rotary evaporation under reduced pressure with a rotary evaporator, so that the density of the concentrated extract is 5 g medicinal material powder / mL.

[0078] (2) Take 5.0 g of each of the three macroporous resins, put them in a conical flask, add 1 mL of the concentrated extract of kaempferia florida above, and 50 mL of distilled water for static adsorption for 24 hours. After observing the appearance of the solution, it can be seen that after 24 hours of static adsorption of NKA-2 resin, the liquid is still relatively turbid, and there is no obvious...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
encapsulation rateaaaaaaaaaa
Login to View More

Abstract

The invention discloses a Krachaidum total flavanone extract as well as a preparation method and application thereof. The method comprises the following steps: by taking dried rhizome of the Krachaidum as a raw material, crushing, extracting, adsorbing, separating and purifying by adopting an AB-8 or HPD300 macroporous resin column, and drying to obtain the Krachaidum total flavanone extract with the purity of over 90 percent. According to the invention, the Krachaidum total flavanone extract is prepared into the Krachaidum total flavanone solid dispersion, the Krachaidum total flavanone extract and the wheat germ oil are combined to obtain the soft capsule, and the soft capsule is formed by a microemulsion and a self-microemulsion preparation, so that the aim of improving the solubility and bioavailability of the Krachaidum total flavanone is fulfilled. The Krachaidum total flavanone extract and wheat germ oil composition prepared by the invention has a synergistic effect, has an anti-aging effect while remarkably enhancing the reproductive function of drosophila melanogaster, and has very high nutritional and medicinal values.

Description

technical field [0001] The invention relates to a total flavonoid extract of kaempferia florida and its preparation method and application, belonging to the technical field of medicine. Background technique [0002] Kaempferia parviflora is a perennial Zingiberaceae plant, mainly produced in northern Thailand, northeast, Laos and Southeast Asia. Kaempferia rhizome, also known as black ginger, has a long history of application and is used to treat a variety of diseases, such as inflammation, ulcers, gout, abscesses, allergies, osteoarthritis, fatigue, gastrointestinal disorders, sexual dysfunction, cancer, high Blood pressure, etc., which are mainly related to the active ingredient methoxyflavonoids, but because the total content of methoxyflavones in medicinal materials is relatively low, in order to better develop and utilize the resources of kaempferia, it is necessary to extract and enrich them Process system research and development. [0003] Chinese patent application...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/906A61K9/107A61K9/48A61K47/44A61P15/08A61P39/06A61K36/899A61K125/00
CPCA61K36/906A61K36/899A61K9/48A61K9/1075A61K47/44A61K47/10A61P15/08A61P39/06A61K2236/333A61K2236/51A61K2300/00
Inventor 刘清飞范雪梅韩福国李格郝艳丽
Owner TSINGHUA UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com