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Chemiluminescence immunoassay kit for quantitatively detecting CEA antigen based on CuInS2@ZnS nanocrystals

A chemiluminescence immunoassay and cuins2 technology, applied in the field of immunodetection, can solve the problems of poor photostability and complex synthesis process, and achieve the effects of strong photostability, simple synthesis method and good photostability.

Pending Publication Date: 2021-11-26
SHANDONG UNIV
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Problems solved by technology

[0006] Aiming at the deficiencies in the prior art, especially the poor photostability of the existing flash-type chemiluminescent immunoassay kits and the complex synthesis process, the present invention provides a CuInS-based 2 Chemiluminescence immunoassay kit for quantitative detection of CEA antigen by @ZnS nanocrystals

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  • Chemiluminescence immunoassay kit for quantitatively detecting CEA antigen based on CuInS2@ZnS nanocrystals
  • Chemiluminescence immunoassay kit for quantitatively detecting CEA antigen based on CuInS2@ZnS nanocrystals
  • Chemiluminescence immunoassay kit for quantitatively detecting CEA antigen based on CuInS2@ZnS nanocrystals

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Embodiment 1

[0052] A CuInS-based 2 Chemiluminescent immunoassay kit for quantitative detection of CEA antigen by @ZnS nanocrystals, including:

[0053] Biotin-labeled CEA mAb 1, streptavidin-labeled magnetic beads, CuInS 2 @ZnS nanocrystal-labeled CEA monoclonal antibody secondary and binary excitation solution;

[0054] The binary excitation solution contains Tris, HCl and N 2 h 4 ·H 2 O and H 2 o 2 .

[0055] CuInS 2 @ZnS nanocrystal-labeled CEA monoclonal antibody II was prepared as follows:

[0056] Take 1 ml of the prepared CuInS 2 @ZnS nanocrystals were centrifuged three times and redissolved in 3 ml of 0.1mol / L MES solution with a pH of 6.0. Add 5.8 μL of 1 mg / mL EDC and 16.3 μL of 1 mg / mL sulfo-NHS to activate the carboxyl group for 15-40 minutes. An additional 2 μL of mercaptoethanol was added to terminate unreacted EDC. Add 300 μL of 10 μg / mL CEA monoclonal antibody II, mix well and react at room temperature for 2 hours, use the amino group on CEA monoclonal antibody ...

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Abstract

The invention relates to a chemiluminescence immunoassay kit for quantitatively detecting a CEA antigen based on CuInS2@ZnS nanocrystals. The kit comprises a biotin-labeled CEA monoclonal antibody I, a streptavidin-labeled magnetic bead, a CuInS2@ZnS nanomaterial-labeled CEA monoclonal antibody II and a binary excitation solution. The binary excitation liquid is prepared from Tris-HCl, N2H4.H2O and H2O2 (hydrogen peroxide). The CuInS2@ZnS nanocrystal can generate flash type chemiluminescence under the excitation of a binary excitation liquid, and the luminescence wave band is located in a near-infrared region. According to the kit, CEA antigen detection is achieved based on the principle that the CuInS2@ZnS nanocrystals can generate flash type chemiluminescence, the kit is high in detection speed, high in sensitivity, good in stability, simple in material synthesis method, high in success rate and good in light stability, and the chemiluminescence wave band is located in a near-infrared region. The kit shows a good linear relation within the concentration range of 50 pg / mL-100 ng / mL, the detection limit is 30 pg / mL, and the kit has a good clinical application prospect.

Description

technical field [0001] The invention belongs to the technical field of immunoassay, and relates to a CuInS-based 2 A chemiluminescence immunoassay kit for the quantitative detection of CEA antigen by @ZnS nanocrystals. Background technique [0002] Chemiluminescent immunoassays include immunochemical reactions and chemiluminescent reactions. Chemiluminescent immunoassay refers to the labeling of chemiluminescent substances on antigens or antibodies, and the specific combination of antigens and antibodies to form immune complexes. The chemiluminescent substances are excited by the excitation solution to form excited state intermediates. The energy in the process of returning to the ground state is in the form of The photons are released in the form of photons, and the luminous intensity is detected by using a photon counter or other instruments. The concentration of the substance to be tested can be related to the luminous intensity to achieve the purpose of detection. [0...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N21/76
CPCG01N33/57473G01N21/76
Inventor 邹桂征董双田
Owner SHANDONG UNIV
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