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Preparation method of floating algae containing pseudoempty cells

A technology of planktonic algae and planktonic algae, applied in the field of preparation of planktonic algae, can solve the problems of cell death, low efficiency, high cost, etc., and achieve the effects of increasing proliferation rate, high biological safety and easy operation

Active Publication Date: 2021-11-30
广州康臣药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to overcome the above-mentioned deficiencies in the prior art, to provide a method for applying planktonic algae containing pseudovacuum and an oral ultrasound contrast agent to solve the problem of unsatisfactory imaging quality of existing gastrointestinal contrast agents and / or There are technical problems of poor biological safety and high cost
[0009] Another object of the present invention is to provide a method for cultivating planktonic algae containing pseudovacua, to solve the technical problems of low efficiency of existing planktonic algae culturing methods, low content of pseudovacua in intracellular air sacs, and easy cell death

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  • Preparation method of floating algae containing pseudoempty cells
  • Preparation method of floating algae containing pseudoempty cells
  • Preparation method of floating algae containing pseudoempty cells

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preparation example Construction

[0037] In yet another aspect, the embodiments of the present invention also provide a method for preparing the planktonic algae containing pseudovacuoles in the above embodiments. The process flow of the method for screening and expanding the cultivation of the planktonic algae containing pseudovacuum is as follows: figure 1 As shown, it includes the following steps:

[0038] Step S01: inoculating planktonic algae containing pseudophyllocysts into the primary planktonic algae culture medium, and injecting sterile oxygen-containing gas into the planktonic algae culture medium for sterile aerobic culture treatment;

[0039] Step S02: After the planktonic algae are cultivated to the logarithmic growth phase, inoculate the planktonic algae in the logarithmic growth phase into the planktonic algae expansion medium for sterile aerobic expansion culture treatment;

[0040] Step S03: statically treat the phytoplankton that has undergone the aseptic aerobic expansion culture, and then...

Embodiment 1

[0064] Embodiment 1: the cultivation method of the planktonic algae containing pseudovacuum

[0065] The present embodiment provides a method for cultivating planktonic algae containing pseudovacuoles, comprising the steps of:

[0066] S11: Cultivation of Anabaena blooms in stagnation period (about 10 days): Open the lid of Anabaena blooms freeze-dried powder (purchased from ATCC, American Biological Standards Collection Center), place in a sterile environment at room temperature for one day to rewarm, Pour the freeze-dried powder into a 50ml Erlenmeyer flask, add cyanobacteria BG11 medium at a volume ratio of 1:50, shake well, insert a ventilation catheter into the Erlenmeyer flask to connect to the air pump, and fill the mouth of the Erlenmeyer flask with sterilized cotton, Seal it with a sealing strip (paraflim) to keep the bottle in a sterile environment; the culture light time is 14h light / 10h dark, the temperature is 25°C, and the shaker speed is 100rpm. After about 6 da...

Embodiment 2

[0070] Embodiment 2: the cultivation method of the planktonic algae containing pseudovacuum

[0071] The present embodiment provides a method for cultivating planktonic algae containing pseudovacuoles, comprising the steps of:

[0072] S11: Cultivation of Microcystis stagnation period (about 10 days): Pour Microcystis into a 50ml Erlenmeyer flask, add cyanobacteria BG11 medium at a volume ratio of 1:50, shake well, and insert a ventilation tube into the Erlenmeyer flask Connect the air pump, fill the mouth of the Erlenmeyer bottle with sterilized cotton, and seal it with a sealing strip (paraflim) to keep the bottle in a sterile environment; the culture light time is 14h light / 10h dark, the temperature is 25°C, and the shaker speed is 100rpm. After about 6 days of culture, when the culture medium changes from the initial colorless and transparent to light green, add an equal amount of fresh BG11 culture medium to continue the culture, and when the green color of the culture me...

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Abstract

The invention provides a preparation method and application of floating algae containing pseudoempty cells. The invention also discloses application of the floating algae containing the pseudoempty cells in preparation of an oral ultrasonic contrast agent. After the floating algae containing the pseudoempty cells are taken as an oral ultrasonic contrast agent, the biological air bag with the pseudoempty cells can effectively play a role of a scattering medium, so that a gastrointestinal wall structure can be clearly developed in a two-dimensional ultrasonic and contrast mode, space-occupying lesions in the stomach can be intuitively found in the contrast mode, the floating algae become an imaging medium in the two-dimensional ultrasonic mode, the adjacent relation between the tumor, the gastrointestinal tract wall structure and the surrounding organs can be clearly displayed, and the biological safety of the floating algae containing the pseudoempty cells is high. Based on the application of the floating algae, the invention provides an oral ultrasonic contrast agent containing the floating algae. According to the culture method of the floating algae, the proliferation rate of the floating algae can be effectively increased, more intracellular pseudoempty-cell airbag structures can grow in floating algae cells, and the imaging effect of the floating algae is improved.

Description

technical field [0001] The invention relates to the technical field of medical detection reagents, in particular to a preparation method and application of planktonic algae containing pseudovacuoles. Background technique [0002] Gastrointestinal diseases mainly include malignant diseases such as acute and chronic gastroenteritis, acute and chronic colitis, peptic ulcer, gastric cancer and colorectal cancer, and the incidence rate accounts for about 20% of the national population. Among them, malignant diseases such as gastrointestinal stromal tumor and gastric cancer will not only seriously affect the lives of patients and their families, but also pose a threat to their lives. Some data show that the 5-year survival rate of gastric cancer that has not spread and metastasized in the early stage can reach about 80% after surgical resection. It can be seen that early diagnosis and timely surgical treatment are crucial to the outcome of the disease. [0003] At present, gastr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12N1/02A61K49/22C12R1/89
CPCC12N1/12C12N1/02A61K49/223Y02A40/80
Inventor 严飞王宇郑海荣
Owner 广州康臣药业有限公司