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Experimental method of [beta]-sitosterol for relieving breast fibrosis diseases

A technology of fibrotic diseases and experimental methods, applied in the field of β-sitosterol in the field of relieving breast fibrosis diseases, can solve the problems of antibiotic residues, insufficient alternative alleviation drugs, etc., to achieve harmless ingredients and not easy to produce drug resistance , does not produce the effect of antibiotic residues

Inactive Publication Date: 2021-12-03
JILIN UNIV
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Problems solved by technology

[0007] According to the current deficiencies about alleviating mammary gland fibrosis, the present invention provides an experimental method for β-sitosterol to relieve mammary gland fibrosis. The effect is obvious, which is conducive to solving the problems of antibiotic residues, drug-resistant bacteria and insufficient alternative relieving drugs in the process of relieving mammary gland fibrosis in dairy cows

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  • Experimental method of [beta]-sitosterol for relieving breast fibrosis diseases
  • Experimental method of [beta]-sitosterol for relieving breast fibrosis diseases
  • Experimental method of [beta]-sitosterol for relieving breast fibrosis diseases

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Embodiment Construction

[0025] Combined with the data provided in this patent, the following will give a complete and accurate description of the technical solutions in the embodiments of the present invention. Other embodiments obtained by other skilled persons in this professional field without making breakthrough achievements all belong to the protection category of the present invention.

[0026] S1. Select 8-week-old ICR mice for co-caging, with 1 male and 4 females in each cage. The mouse mammary gland fibrosis model will be established within 3 to 7 days after the mice are delivered. The experiment is divided into 3 groups, each of which is blank Control group (NT), mammary gland fibrosis model group (TGF-β1), drug relief group (TGF-β1+β-sitosterol), 5 mice in each group.

[0027] S2. Injecting β-sitosterol intraperitoneally to mice once, the administration concentration is 50 mg / kg, and the administration time is 1 hour before each injection of TGF-β1.

[0028] S3. Take out the fresh mammary...

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Abstract

The invention relates to an experimental method of [beta]-sitosterol for relieving breast fibrosis diseases. The experimental process is as follows: S1, selecting ICR mice 3-7 days after delivery as experimental subjects in the experiment, dividing the mice into three groups, namely a blank control group (NT), a mammary gland fibrosis model group (TGF-[beta]1) and an administration remission group (TGF-[beta]1+[beta]-stosterone), wherein each group comprises five mice; and S2, anesthetizing and fixing the mouse by using pentobarbital sodium, injecting TGF-[beta]1 into the fourth pair of milk regions of the mouse every three days to construct a breast fibrosis model, totally five cycles, and collecting a sample within 24 hours after the model is constructed. According to the experimental method of [beta]-sitosterol for relieving breast fibrosis diseases, the damage condition of breast tissue is evaluated through an H&E dyeing technology, the fibrosis condition of the breast tissue is evaluated through an Msaaon dyeing technology, and the expression quantity of Collagen II in the breast tissue is detected through adoption of a Westernblot method. Experimental results can prove that the [beta]-sitosterol has an effect of relieving breast fibrosis.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to an experimental method for beta-sitosterol used to alleviate mammary gland fibrosis. Background technique [0002] Dairy cow mastitis is one of the most common diseases of dairy cows. Dairy cow mastitis leads to a significant decline in milk production. In severe cases, no milk will eventually lead to the elimination of dairy cows, causing huge economic losses to the dairy industry. The pathogens that cause mastitis in dairy cows include viruses, bacteria, mycoplasma and fungi, etc., but most of them are bacterial infections, among which Escherichia coli, Staphylococcus aureus and streptococcus are the main ones, accounting for more than 90% of the pathogenic bacteria of mastitis. Cows with mastitis in clinical practice often have partial or general sclerosis of the mammary glands, but this phenomenon has not received enough attention. [0003] Mammary gland fibrosis in dairy c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/575A61P15/14A61P29/00G01N33/68
CPCA61K31/575A61P15/14A61P29/00G01N33/6893G01N2800/365
Inventor 付守鹏徐平柳巨雄阚兴池郭文晋曹宇
Owner JILIN UNIV
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