Fujian mycoplasma synoviae strain and culture medium thereof

A technology of Mycoplasma synovialis and culture medium, which is applied in the field of pathogen isolation and identification and pathogen culture, can solve the problems of difficulty in eradicating Mycoplasma synovialis in chickens, increase mortality, cross infection, etc. The effect of mycoplasma, simple culture method, stable pathogenic characteristics

Active Publication Date: 2021-12-07
FUJIAN AGRI & FORESTRY UNIV
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

Mycoplasma synovialum can directly invade the cartilage tissue of chickens, causing joint swelling and swelling, inflammation of synovial bursa and tendon sheath, lameness and paralysis of chickens; Mycoplasma synovialum infection can also reduce egg production rate and egg quality of laying hens and hatching rate, reduce the feed conversion rate, increase the waste rate of ketone bodies, and Mycoplasma synovialum can spread vertically, which brings great difficulties to the eradication of Mycoplasma synovialum in chicken flocks
At present, Mycoplasma synovialum is widely present in chicken farms all over the world, and Mycoplasma synovialum is prone to cross-infection with other pathogens, aggravating the pathogenicity of each pathogen, increasing the mortality rate, and causing serious economic losses to the chicken industry

Method used

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  • Fujian mycoplasma synoviae strain and culture medium thereof
  • Fujian mycoplasma synoviae strain and culture medium thereof
  • Fujian mycoplasma synoviae strain and culture medium thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Embodiment 1, the isolation and identification of a strain Mycoplasma gallisanum bursa

[0027] (1) Isolation of Mycoplasma bursae

[0028] Collect the joint fluid and content in the swollen tarsal joint cavity of chickens suspected of being infected with Mycoplasma gallisanum from Fuzhou chicken farms, inoculate it in the modified Frey's liquid medium under aseptic conditions, and place it at 37°C for 48 hours of shaking culture , when the bacterial liquid turns from red to yellow, filter the bacterial liquid with a 0.45 μm filter, and re-inoculate the modified Frey’s liquid medium at a volume ratio of 1:10 between the filtrate and the modified Frey’s liquid medium. Cultivate and collect the second-generation bacterial liquid for identification of pathogenic bacteria.

[0029] (2) Identification of Mycoplasma bursae

[0030] 1. Bacterial morphology identification

[0031] Smear the isolated second-generation bacterial liquid, and microscopically examine it after Gie...

Embodiment 2

[0049] Embodiment 2, the preparation of chicken synovial bursa mycoplasma culture medium

[0050] (1) Preparation of different formulas of Mycoplasma gallinarum liquid medium

[0051] 1. Take brain heart extract broth, yeast extract powder, phenol red solution, L-cysteine ​​solution, arginine solution and coenzyme I solution, mix and dissolve, and dilute to 900 ml with deionized water.

[0052] 2. Use hydrochloric acid to adjust the pH value of the above culture medium to 7.6~7.8.

[0053] 3. Add 100 ml of inactivated fetal bovine serum to the above medium under aseptic conditions, and filter the medium into a new sterile container with a 0.22 μm bacterial filter, and finally add 800,000 units / L of penicillin Finish the preparation.

[0054] Four liquid culture media for Mycoplasma gallinae bursa with different proportions of ingredients were prepared, and the specific schemes are shown in Table 1, so as to screen out the best scheme for culture medium for Mycoplasma gallina...

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Abstract

The invention provides a mycoplasma synoviae strain and a culture medium thereof. The mycoplasma synoviae strain is separated from sick chicken attached joints with chicken mycoplasma synoviae in Fuzhou and belongs to typical strains in Fujian province. The strain is preserved in the China Center for Type Culture Collection, the preservation address is Wuhan University, Wuhan, China, the preservation number is CCTCC NO:M 2021210, and the preservation date is March 8, 2021. A culture medium for culturing the mycoplasma synoviae strain comprises the following components: brain heart leachate broth, yeast extract powder, inactivated fetal calf serum, an L-cysteine solution, an arginine solution, a phenol red solution and a coenzyme I solution, and the pH value of the culture medium is 7.6-7.8. The mycoplasma synoviae strain is inoculated to the culture medium and placed in an incubator at the temperature of 37 DEG C to be subjected to static culture for 36 h, the growth peak value of the mycoplasma synoviae strain can be reached, and the viable count of the cultured mycoplasma synoviae is not lower than 10<12> CCU / ml.

Description

technical field [0001] The invention belongs to the technical field of pathogen isolation and identification and pathogen cultivation, and in particular relates to a strain of mycoplasma bursae and a culture medium thereof. Background technique [0002] Mycoplasma synoviae (MS) is an acute or chronic infectious disease that mainly causes respiratory symptoms and infectious synovitis symptoms in chickens and turkeys. The disease can occur in all seasons, but mainly in winter and spring Frequent. Mycoplasma synovialum can directly invade the cartilage tissue of chickens, causing joint swelling and swelling, inflammation of the synovial bursa and tendon sheath, lameness and paralysis of chickens; Mycoplasma synovialum infection can also reduce the egg production rate and egg quality of laying hens. and hatching rate, reduce feed conversion rate, increase ketone waste rate, and Mycoplasma synovialum can spread vertically, which brings great difficulties to the eradication of My...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N15/11A61K39/02A61P31/04C12Q1/689C12Q1/04C12R1/35
CPCC12N1/20C12Q1/689A61K39/0241A61P31/04
Inventor 李训良陈吉龙许李锋
Owner FUJIAN AGRI & FORESTRY UNIV
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