Biosynthesis method of starch

A technology of amylose and synthetic starch, which is applied in the direction of transferase and fermentation, can solve the problems of lack of technology and methods, and achieve the effect of reducing agricultural land and shortening the synthesis cycle

Pending Publication Date: 2021-12-07
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no technology and method that can meet the needs of both aspects at the same time.

Method used

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  • Biosynthesis method of starch
  • Biosynthesis method of starch

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0172] Embodiment 1 is from formic acid or methyl alcohol to the synthesis of compound C i.e. formaldehyde

[0173] There are five pathways for the conversion of formic acid or methanol to compound C, namely: pathway 1 (from methanol to formaldehyde), pathway 2 (from methanol to formaldehyde), pathway 3 (from formic acid to formaldehyde), pathways 4-7 (from formate to formyl-CoA to formaldehyde), and pathway 5-6-7 (from formate to formyl phosphate to formyl-CoA to formaldehyde) (reaction numbers see figure 1 ). Catalysts (ie, enzymes) that can catalyze each chemical reaction of each pathway are first selected (see Table 1), but enzymes with corresponding catalytic functions are not limited to those listed in Table 1. Then different catalysts are combined according to the route to establish a corresponding reaction system, and after a period of reaction, the production of formaldehyde is detected.

[0174] The output of compound C was detected according to the following metho...

Embodiment 2

[0180] Embodiment 2 is from methyl alcohol to the synthesis of compound D i.e. dihydroxyacetone

[0181] The synthesis from methanol to compound D can be achieved through the following routes:

[0182] Route 2-8: The reaction system is Hepes buffer 100mM at pH 7.5, NaCl 100mM, Mg 2+ 5mM, Zn 2+ 10μM, AOX 1U / mL, CAT 300U / mL, methanol 20mM, FLS 5mg / mL; after 2 hours of reaction, the yield of compound D was 2.1mM.

[0183] The detection method of compound D: 5mM dilute sulfuric acid as mobile phase, HX87 column (Bio-Rad, Aminex@, 300mm×78mm), flow rate 0.6mL / min, sample volume 10μL / needle, calculate compound D according to DHA standard curve Yield. Figure 4 The yields of pathways 2-8 from methanol to compound D are shown in .

Embodiment 3

[0184] Embodiment 3 is from formaldehyde to the synthesis of compound D i.e. dihydroxyacetone

[0185] The synthesis from formaldehyde to compound D can be realized through the following routes:

[0186] Route 8: The reaction system is Hepes buffer 100mM at pH 7.5, NaCl 100mM, Mg 2+ 5mM, Zn 2+ 10 μM, formaldehyde 25 mM, TPP 0.5 mM, FLS (source: Pseudomonasputida) 10 mg / mL; react for 2 hours, and use the method in Example 2 to detect compound D. Results: The yield of compound D synthesized by formaldehyde lyase FLS is 7.092 mM.

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Abstract

The invention provides a biosynthesis method of starch. The biosynthesis method can realize full-artificial biosynthesis of the starch from simple compounds such as dihydroxyacetone, formaldehyde, formic acid and methanol, and even can realize full-artificial biosynthesis of the starch by coupling with methods such as chemical reduction of carbon dioxide and the like, and even taking the carbon dioxide as an initial raw material. The synthesis of the natural starch needs Kalvin cycle, 21-22 steps of reaction are needed in total, while the biosynthesis method only needs 9-12 steps of reaction, nearly half of reaction steps are reduced, and the production cycle can be greatly shortened. According to the biosynthesis method of the starch, besides, high-concentration and high-density carbon dioxide and high-energy-density electric energy and hydrogen energy can be utilized in the biosynthesis method, the biosynthesis method is more suitable for an industrial production mode, and the production cycle can be shortened from several months of agricultural planting to several days.

Description

technical field [0001] The invention belongs to the technical field of biosynthesis, and in particular relates to a method for biosynthesis of starch (including amylose and amylopectin). Background technique [0002] Global food production needs to consume 38% of land and 70% of fresh water resources. With the increase of global population, it is predicted that the global food demand will increase by 50-70% by 2050. The current agricultural planting methods are difficult to meet the growing food demand . Starch is an important food raw material, providing about half of the daily energy required by the human body. In addition, starch is also an important raw material for biomanufacturing and production of chemicals, for example, it can be used to produce amino acids, organic acids, bioethanol, etc. [0003] Agricultural cultivation is currently the only way to produce starch. The natural synthesis pathway of starch in crops includes the Calvin cycle, involving a total of t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12P19/24C12P19/18C12P7/26
CPCC12P19/04C12P19/24C12P19/18C12P7/26C12P19/14C12N9/0006C12N9/0008C12N9/93C12N9/1217C12N9/1029C12N9/88C12N9/1205C12N9/90C12N9/16C12N9/1241C12N9/1051C12N9/0065C12N9/0093C12Y101/01001C12Y101/03013C12Y102/01003C12Y602/01001C12Y207/02001C12Y203/01008C12Y102/01005C12Y401/01007C12Y207/01029C12Y401/02013C12Y301/03011C12Y402/01C12Y503/01009C12Y504/02008C12Y207/04027C12Y204/01021C12Y204/01001C12Y204/01018C12Y111/01006C12Y117/01C12Y306/01001C12N15/52C12N9/10C12Y101/01103C12Y101/01244C12Y101/01245C12Y101/03006
Inventor 马延和蔡韬孙红兵乔婧张璠张洁王钦宏
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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