Radix morindae officinalis processed product oligosaccharide composition, and preparation method and application thereof

A composition and technology of Morinda officinalis salt are applied in the field of oligosaccharide composition of processed Morinda officinalis and its preparation, and can solve the problems of restricting the application of processed Morinda officinalis products and the like.

Inactive Publication Date: 2021-12-10
GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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Problems solved by technology

The literature "Research on the effect of Morinda officinalis oligosaccharides on mouse spermatogenesis" (Chinese Journal of Pharmaceutical Sciences, 2008, 43(19): 1467-1470) discloses the research on the effect of oligosaccharides in Morinda officinalis products on mouse spermatogenesis , which only mentions that "the material basis of Morinda officinalis to promote spermatogenesis in mice may be the extract of total oligosaccharides from Morinda officinalis"; CN106668058A discloses the repairing effect of Morinda officinalis polysaccharides on male reproductive system damage, and CN102198184A discloses The extract of Morinda officinalis has the effect of improving or treating sexual dysfunction, and the extract contains oligosaccharides and polysaccharides of Morinda officinalis, and the sum of the content is greater than 50%; the oligosaccharides or polysaccharides are all obtained from the natural products of Morinda officinalis Extracted; "Research on Oligosaccharide Components of Morinda officinalis" (Feng Feng. Research on oligosaccharide components of Morinda officinalis [D]. Guangzhou University of Traditional Chinese Medicine, 2011.) discloses the specific composition of 6 oligosaccharides in the extract of Morinda officinalis , structural formula, but the research object is also the herbal medicine of Morinda officinalis, and currently there are few studies on the structure of oligosaccharides after processing Morinda officinalis, and there are very few studies on the application of oligosaccharides from Morinda officinalis processed products. Limiting the application of Morinda officinalis processed products in health care products and medicines

Method used

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  • Radix morindae officinalis processed product oligosaccharide composition, and preparation method and application thereof
  • Radix morindae officinalis processed product oligosaccharide composition, and preparation method and application thereof
  • Radix morindae officinalis processed product oligosaccharide composition, and preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0096] The preparation of embodiment 1 Morinda officinalis processed product oligosaccharide

[0097] 1. Preparation of oligosaccharides from various processed products of Morinda officinalis

[0098] 1.1 Preparation of Raw Morinda officinalis and processed products of Morinda officinalis

[0099] Take fresh Morinda officinalis, remove the soil and fibrous roots, remove the wood core, and dry at 60°C to obtain raw Morinda officinalis;

[0100] Take 1000g of raw Morinda officinalis, cut it into short sections of 1-1.5cm, add 700mL of water and moisten it for 4 hours until the water is completely absorbed, and steam it in a stainless steel pot at 160°C for 4h, take it out, let it cool, dry at 60°C, and sieve Remove the debris, and you can get the processed product of Morinda officinalis: Morinda officinalis meat;

[0101] Take 1000g of raw Morinda officinalis, cut it into short sections of 1-1.5cm, add 700mL of saline with 3% of the medicinal material, moisten it for 4 hours u...

Embodiment 2

[0108] Example 2 Determination of oligosaccharide components of raw Morinda officinalis and processed products of Morinda officinalis

[0109] Take 0.1 g of oligosaccharides of raw Morinda officinalis, Morinda officinalis meat, and Morinda officinalis salt, accurately weigh the weight, add 60% acetonitrile to dissolve and dilute to 10 mL, shake well, pass through a 0.22 μm microporous membrane, as Samples to be tested; 6 kinds of inulin-type oligosaccharides were taken as reference substances: D-fructose, D(+)-anhydroglucose, sucrose, 1-kestose, nassose and 1 F - Fructofuranosyl Nessose, accurately weighed, placed in a measuring bottle, added 60% volume fraction of acetonitrile to dissolve and set the volume to 10mL, shake the solution, and make each 1mL containing D-fructose 1.378mg, D- (+)-Anhydrous Glucose 0.828mg, Sucrose 1.210mg, 1-Kestose 0.832mg, Nessose 1.280mg, 1 F -The mixed reference substance solution of 1.658 mg of fructofuranosyl Nessose.

[0110] Both the test...

Embodiment 3

[0112] Example 3 Separation and identification of oligosaccharide component X in Morinda officinalis processed products

[0113] 1. Separation of oligosaccharide component X in Morinda officinalis processed products

[0114] 1.1 Preparation of acetylated oligosaccharides from Morinda officinalis processed products

[0115] (1) Get 15g of oligosaccharides of Morinda officinalis Morinda officinalis processed product salt Morinda officinalis of embodiment 1, join in 500mL dry round bottom flask, add pyridine 200mL, stir at room temperature to make oligosaccharides all dissolve, then add acetic anhydride 200mL, Shake well and react at room temperature for 36h. The mixture was concentrated under reduced pressure to obtain acetylated oligosaccharide extract.

[0116] (2) Take 25g of Morinda officinalis processed product oligosaccharide acetylate extract, add appropriate amount of methanol to dissolve it completely, add 50g of 100-200 mesh silica gel and stir, make the silica gel e...

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Abstract

The invention discloses a radix morindae officinalis processed product oligosaccharide composition, and a preparation method and application thereof. The invention carries out separation on a radix morindae officinalis processed product to obtain a radix morindae officinalis processed product oligosaccharide composition.The oligosaccharide composition consists of Inulo type oligosaccharide and Inulin type oligosaccharide. According to the radix morindae officinalis processed product salt radix morindae officinalis oligosaccharide, the content of MDA can be remarkably reduced, and the activity of SOD, GSH-Px and CAT is enhanced to protect TM4 oxidative stress damage induced by H2O2. According to the radix morindae officinalis processed product radix morindae officinalis oligosaccharide, the content of MDA can be remarkably reduced, the activity of GSH-Px and CAT is enhanced, mouse germ cell oxidative damage caused by cyclophosphamide is protected, and reduction of the sperm malformation rate of male mice with spermatogenesis disorder caused by cyclophosphamide and improvement of sperm generation and sperm quality can be remarkably promoted. The radix morindae officinalis processed product oligosaccharide composition can be used for preparing health-care products or medicines for preventing or treating male reproductive system diseases.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine, and more specifically relates to a processed oligosaccharide composition of Morinda officinalis and its preparation method and application. Background technique [0002] Morinda officinalis is derived from the dry root of Morinda officinalis How, a plant of Rubiaceae, and is one of the famous "four southern medicines" in my country. It is mainly distributed in Guangdong, Guangxi, Fujian, Hainan, Jiangxi and other areas south of the Yangtze River Basin. Among them, Deqing, Guangdong is a recognized production area of ​​Morinda officinalis. The medicine is slightly warm in nature, sweet and pungent, and has the effects of nourishing liver and kidney, strengthening muscles and bones, and dispelling rheumatism. It is one of the traditional Chinese medicines for nourishing kidney and yang in my country. disease. According to different processing methods, Morinda officinalis is di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/702A61K31/715A61P15/08C07H3/06C07H1/08C08B37/00A23L33/125
CPCA61K31/702A61K31/715A61P15/08C07H3/06C07H1/08C08B37/006C08B37/0003A23L33/125A23V2002/00A61K2300/00A23V2200/30
Inventor 丁平刘梦云
Owner GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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