Bacillus velezensis HYL-1 and application thereof

A technology of Bacillus and Velez, applied in the field of microorganisms, can solve problems such as banana fusarium wilt, and achieve the effects of improving soil, promoting banana growth and wide application fields.

Active Publication Date: 2021-12-17
GUANGXI ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Fusarium oxysporum f.sp. Cubense (Foc) is a soil-borne fungus that causes wilt of bananas and is considered is the most devastating disease

Method used

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  • Bacillus velezensis HYL-1 and application thereof
  • Bacillus velezensis HYL-1 and application thereof
  • Bacillus velezensis HYL-1 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Screening of strain Bacillus velezensis HYL-1:

[0049] Bacillus velezensis HYL-1, with the preservation number GDMCCNO.61927, is preserved in: Guangdong Microbial Culture Collection Center, address: 5th Floor, Building 59, Compound, No. 100 Xianlie Middle Road, Guangzhou, and the preservation date is 2021 September 10th.

[0050] The above-mentioned strains were isolated in 2021 from the soil of a banana cultivation field at the farm of Xinkejian Biotechnology Co., Ltd. in Nanning, Guangxi Zhuang Autonomous Region.

[0051] The separation method is: dissolve the soil in sterile water, after homogeneous suspension, take 100 μl of the suspension and spread it on a petri dish containing a selective medium, incubate the petri dish at 28°C for up to 1 day, and then calculate the colony forming units . Pick each bacterial colony separately from all tissue samples and transfer to growth medium plates. After keen observation and subculture, pure bacteria are isolated based ...

Embodiment 2

[0059] This example mainly studies the inhibitory effect of bacterial strain HYL-1 on banana Fusarium wilt.

[0060] 1. The confrontation experiment between strain HYL-1 and pathogenic bacteria (Foc1 and Foc4), the specific experimental process is as follows:

[0061] ①Preparation of pathogenic bacteria suspension: add the agar medium with pathogenic bacteria to the PDB liquid medium, culture at 28°C and 170rpm for 7 days, filter the suspension through four layers of gauze after the cultivation, and then adjust the final concentration with distilled water to 10×10 6 cfu / ml, spare.

[0062] ② Inoculate the suspension of the above-mentioned pathogenic bacteria on the PDA medium, in which the control group was only inoculated with the pathogenic bacteria of the new strain of the application; while the experimental group was inoculated with the pathogenic bacteria on one side of the PDA medium, and the corresponding HYL- 1 strain for antagonistic experiments.

[0063] image 3...

Embodiment 3

[0095] Inhibition of bacterial wilt by secondary metabolites of strain HYL-1:

[0096] ① Inoculate R. solanacearum into LB liquid medium, shake and culture at 37°C and 180rpm for 16h, set aside;

[0097] ②Preparation of culture supernatant of strain HYL-1:

[0098] The HYL-1 strain was inoculated in LB liquid medium, and shaken at 37°C and 180rpm for 24h. Then the culture solution was centrifuged at 3500rpm for 10min, and placed in a 4°C refrigerator for later use;

[0099] ③ Preparation of pathogen indicator plate:

[0100] Place 2 Oxford cups symmetrically on the LB solid medium plate, mix R. solanacearum with the LB agar medium that has been melted and cooled to 50°C at a ratio of 1:100 and pour 20ml into the upper surface of the above LB solid medium. After coating evenly and cooling and solidifying, use sterile tweezers to remove the Oxford cup to obtain a medium with holes;

[0101] ④ Sample loading:

[0102] Experimental group: pipette 100 μL of the supernatant of ...

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Abstract

The invention relates to the technical field of microorganisms, in particular to bacillus velezensis HYL-1 and application thereof. The bacillus velezensis HYL-1, fungicide and metabolite of the bacillus velezensis HYL-1 have wide antibacterial and disease-resistant effects, can be used for preventing and controlling siraitia grosvenorii southern blight and banana wilt, and also have the effects of promoting banana growth, accelerating banana flowering phase and improving banana bud germination; the strain also can synthesize IAA, generate siderophores and dissolve inorganic phosphorus and silicate; and in addition, the strain can generate proteolytic enzyme and amylolytic enzyme. Therefore, the strain provided by the invention is a strain with multiple functions and wide application fields, and has good prospects in the fields of later biological prevention and control, biological bacterial fertilizers, food processing and the like.

Description

【Technical field】 [0001] The invention relates to the technical field of microorganisms, in particular to Bacillus velei strain HYL-1 and its application. 【Background technique】 [0002] Fusarium oxysporum f.sp. Cubense (Foc) is a soil-borne fungus that causes wilt of bananas and is considered the most destructive disease of bananas for which there is currently no effective chemical Agents for prevention and treatment, researchers hope to use biological methods to control banana wilt. Using microorganisms to reduce the use of chemical fertilizers and pesticides without affecting plant yields is an important research field in agricultural biotechnology and microbiology. [0003] Among microorganisms, there is a class of plant growth promoting rhizobacteria (PGPR) that can improve plant growth, increase soil fertility, increase yield and reduce pathogen or abiotic stress. It has been reported in the prior art that Bacillus is used as a biocontrol agent against a number of so...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P1/04A01N63/22A01P1/00A01P3/00A01P21/00B09C1/10C12R1/07
CPCC12N1/20C12P1/04A01N63/22B09C1/10
Inventor 周礼芹王小虎贺瑜岚韦圣博朱绮霞叶柳健何双银梦王睿兰宝峰岑锦艺
Owner GUANGXI ACAD OF SCI
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