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Non-toxic aspergillus flavus XZCY1805 and application thereof

A technology of XZCY1805 and Aspergillus flavus strain, applied in the field of microorganisms, can solve problems such as hidden risks, increased cyclopiazonic acid content, mycotoxin contamination, etc., and achieve the effects of high safety, strong competitive inhibition ability, and strong competitive advantage

Pending Publication Date: 2021-12-28
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing disclosures are non-toxin-producing Aspergillus flavus and uses thereof (such as CN 110157626 A, CN 110305796 A, CN 110129212 A, CN 107177516, CN104789480 A) for biological control, but only aim at not producing aflatoxin, and all producing aflatoxin Aspergillus, cyclopiazonic acid, aflatoxin, aflatoxin, and other toxic metabolites have been reported on biocontrol bacteria with high safety. These strains may bring cyclopiazonic acid, aflatoxin, etc. Risk of contamination with mycotoxins such as tremorin
It has been found that the content of cyclopiazonic acid in corn and peanuts increased after the non-toxigenic Aspergillus flavus AF36 (Afla- / CPA+) was applied as a biocontrol agent, which brought new risks.

Method used

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  • Non-toxic aspergillus flavus XZCY1805 and application thereof
  • Non-toxic aspergillus flavus XZCY1805 and application thereof
  • Non-toxic aspergillus flavus XZCY1805 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Isolation, purification and identification of bacterial strain XZCY1805

[0030] More than 2,000 peanut and soil samples were collected from the main peanut-producing areas in Northeast China, North China, Yangtze River Basin and South China, and 1,600 strains of Aspergillus flavus were obtained through separation, purification and identification; 97 strains were selected after the aflatoxin-producing ability of the strains was determined. Non-toxin-producing Aspergillus flavus (mainly isolated from 15 peanut planting areas including Henan, Shandong, Hubei, Jiangxi, and Tibet), the competition inhibition effect research and the evaluation of toxin-producing ability were carried out: co-tested with the toxin-producing standard strain 3.4408 on peanut powder. After cultivation and evaluation of toxin-producing inhibitory effects, 11 strains with significant inhibitory effect on toxigenic Aspergillus flavus were initially screened out; An, Tibet Chayu, Hubei Hon...

Embodiment 2

[0050] Example 2: Analysis of the toxin production of bacterial strain XZCY1805

[0051] 1. Strain toxin-producing culture

[0052] The non-toxigenic Aspergillus flavus strain XZCY1805 was inoculated on DG-18 solid medium for activation. After cultivating for 5 days at 28±1°C in the dark, the Aspergillus flavus conidia on the plate were washed with sterilized 0.1% Tween 80 to obtain a spore suspension. Determine the concentration of the spore suspension with a hemocytometer. Get a certain amount of spore liquid and inject in the Erlenmeyer flask that sterilized 30ml liquid Sabouraud medium is housed, make its final concentration be 4 * 10 5 pieces / ml. Place the Erlenmeyer flask on a shaker at 28±1°C, 200rpm in the dark.

[0053] 2. Determination of strain toxin content

[0054] After 5 days, use sterilized gauze to filter the mycelial culture solution, discard the mycelium ball, collect the toxin-producing culture solution and store it in a centrifuge tube, let it stand f...

Embodiment 3

[0062] Embodiment 3: the inhibitory effect of bacterial strain XZCY1805 on toxin-producing Aspergillus flavus on peanut powder

[0063] 1. Preparation of spore suspension

[0064] The non-toxigenic Aspergillus flavus strain XZCY1805, the toxigenic Aspergillus flavus standard strain 3.4408, and the toxigenic Aspergillus flavus isolated from different regions are numbered SCPA-32-12 (Pengan, Sichuan), XZCY- 24-6 (Chayu, Tibet), LNFX-25-1 (Fuxin, Liaoning), HBHA-129-1 (Hong'an, Hubei), HBYL-12-7 (Yangluo, Hubei), HBTS-94-2 (Tangshan, Hebei) and JXZS-118-9 (Jiangxi camphor tree) were respectively inoculated in DG-18 solid medium for activation. After culturing for 5 days at 28±1° C. in the dark, wash the conidia of Aspergillus flavus on the plate with 0.1% Tween 80 that has been sterilized and put them in a 10 ml centrifuge tube to obtain a spore suspension. Determine the concentration of the spore suspension with a hemocytometer under a microscope, and dilute the spore concentr...

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Abstract

The invention relates to a non-toxic aspergillus flavus strain and application thereof. A non-toxic aspergillus flavus XZCY1805 disclosed by the invention does not produce various toxins such as aflatoxin (AFT), cyclopiamic acid (CPA), aspergillus flavus tremorine (Aflatrem), sterigmatocystin (ST) and the like, and is a biocontrol strain with high safety and strong competitive advantage. The non-toxic aspergillus flavus XZCY1805 disclosed by the invention has a strong toxic inhibition effect on a standard toxic aspergillus flavus strain 3.4408 and toxic aspergillus flavus separated from different peanut production places, and can remarkably reduce the contents of the aflatoxin, the cyclopiamic acid, the aspergillus flavus tremorin and the sterigmatocystin in agricultural products such as peanuts and the like.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a non-toxin-producing Aspergillus flavus XZCY1805 and its application. Background technique [0002] Aflatoxins (AFT) are mainly secondary metabolites produced by fungi such as Aspergillus flavus and Aspergillus parasiticus after infecting host plants, and are the most toxic contaminated agricultural products found so far. Mycotoxin, with acute and chronic toxicity, can cause cancer, teratogenic, mutagenic. Common types of aflatoxin mainly include aflatoxin B 1 (AFB 1 ), aflatoxin B 2 (AFB 2 ), aflatoxin G 1 (AFG 1 ), aflatoxin G 2 (AFG 2 ). The most toxic and carcinogenic is aflatoxin B 1 , its toxicity is 10 times that of potassium cyanide, 68 times that of arsenic, and its carcinogenicity is 10,000 times that of BHC. Listed as a Class I carcinogen. In addition to aflatoxins, Aspergillus flavus can also produce toxic secondary metabolites such as ...

Claims

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Application Information

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IPC IPC(8): C12N1/14A23L5/20A01N63/34A01P3/00A01G7/06A01G13/00C12R1/67
CPCC12N1/14A23L5/28A01N63/34A01G7/06A01G13/00
Inventor 张奇白艺珍岳晓凤李培武
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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