Method for extracting sophoricoside, genistein and kaempferol from fructus sophorae

Abstract

The invention provides a method for extracting sophoricoside, genistein and kaempferol from fructus sophorae. The method mainly comprises the following steps: firstly, extracting effective components by using methanol or ethanol, then separating the sophoricoside according to the dissoluvability difference of all the effective component in the methanol or ethanol, then hydrolyzing a filtrate twice, performing first-time hydrolyzing at temperature of about 80 DEG C to separate out the kaempferol, further performing second-time hydrolyzing on the filtrate at about 95 DEG C to separate out a mixture of the kaempferol and the genistein, separating out the kaempferol by utilizing the dissoluvability difference of the mixture in alkaline water, further adjusting the pH of the filtrate with acid water, and finally separating out the genistein. According to the method of the invention, a suitable solvent is screened out, the extraction step and the purification process are optimized according to the dissolvability difference of all the effective components, the problem of data waste caused by the fact that three products cannot be comprehensively developed in the prior art is solved, the production cost is reduced, and the operability is improved.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine chemistry, in particular to a method for extracting sophorin, genistein and kaempferol from Sophora japonica. Background technique [0002] Sophora japonicus is the ripe fruit of Sophora japonica, a leguminous plant, with a bitter taste and cold nature. Return liver, large intestine meridian, have the effects of cooling blood for hemostasis, clearing liver and improving eyesight. Sophora japonicus is rich in flavonoids, such as sophorin, genistein and kaempferol. Sophorinoside is an isoflavone glycoside with antifertility, anti-inflammatory, anticancer and immunosuppressive properties. The genistein and kaempferol proposed from Sophorae japonicus have the effects of interfering with the transportation of pregnant eggs, terminating the implantation of pregnant eggs and anti-early pregnancy. And studies have shown that a small amount of long-term administration of genistein a...

AI Technical Summary

Problems solved by technology

[0003] At present, the existing technology is to extract a single active ingredient, and there is a lack of extraction methods for three active ingredients, resulting in a waste of data

In addition, the extraction of sophoridoside involves the separation method of macroporous resin, which requires the use of a large amount of solvent, and the operation is time-consuming and laborious, and the efficiency is low

For the extraction of genistein, enzymatic hydrolysis is mainly used, which has a long production cycle and low conversion rate; column chromatography is also required to prolong the entire production cycle; and the method of formic acid hydrolysis in advance will make the extraction and emulsification serious , making it difficult to separate

There are relatively few studies on the extraction of kaempferol, and the methods of macroporous resin separation and column chromatography purification are mainly used. The amount of solvent used is large, the cost is high, and the extraction takes a long time

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