Application of culture medium in oudemansiella raphanipies

The technology of an original culture medium and culture medium, which is applied to the application in the field of the cultivation medium, can solve the problems such as the limitation of development and utilization, the toxicity of antioxidants, and the insecurity of the cockroach. Theoretical significance and application value, the effect of promoting accumulation and improving protein content

Active Publication Date: 2022-01-07
YANGTZE NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the development and utilization of the relevant P. nigricans is greatly restricted.
In view of the toxicity and unsafety of traditionally synthesized antioxidants, extracting non-toxic and safe antioxidant active substances fro

Method used

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  • Application of culture medium in oudemansiella raphanipies
  • Application of culture medium in oudemansiella raphanipies
  • Application of culture medium in oudemansiella raphanipies

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0029] A kind of cultivation method of P. nigella comprises the following steps:

[0030] 1) Preparation of culture medium

[0031]The stock medium includes the following components: wheat bran 35g / L, glucose 20g / L, dipotassium hydrogen phosphate 3.5g / L, magnesium sulfate 2g / L, agar 20g / L, pH 6.5. Weigh the required wheat bran, glucose, dipotassium hydrogen phosphate and magnesium sulfate respectively according to the formula. Wrap the weighed wheat bran with 6-8 layers of gauze and put it into a cooking pot, add 200mL of water to boil, then time it for 15-30 minutes, then filter it with 6-8 layers of gauze, pour the filtrate into a 500mL large beaker, Add other weighed medicines one by one, stir with a glass rod until dissolved, measure enough water and pour it into a beaker to make up the volume. Use 1 mol / L hydrochloric acid solution to adjust the pH of the culture medium to 6.5, divide into test tubes, seal them, put them in a high-pressure steam pot for sterilization, a...

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Abstract

The invention discloses an application of a culture medium in oudemansiella raphanipies. The culture medium comprises the following components of, in percentage by mass, 35%-55% of vinasse, 10%-30% of cottonseed hull, 10%-20% of wheat bran, 1%-2% of lime, 1% of gypsum and the balance corncob. According to the culture medium, through the synergistic effect of the components, not only can the nutritional and physiological requirements of the growth of the mycelia of the oudemansiella raphanipies be met, but also the accumulation of the nutritional ingredients and effective antioxidant active substances of the oudemansiella raphanipies is promoted to different degrees, the highest contents of protein, total sugar and polyphenol can reach 42.78%, 23.54% and 4.19 mg/g respectively, the scavenging rates of the polyphenol extract on DPPH free radicals, ABTS free radicals and hydroxyl free radicals are 88.64%, 99.81% and 93.48% respectively, the scavenging rates are obviously higher than those of previous research results, therefore, corresponding culture medium formulas are selected according to different use purposes in production, and a theoretical basis is provided for large-scale production of the high-quality oudemansiella raphanipies and development and utilization of functional products of the high-quality oudemansiella raphanipies.

Description

technical field [0001] The invention relates to the technical field of agricultural production, in particular to the application of a cultivation medium for Gallium fruticosa. Background technique [0002] P. black skin, also known as the long-rooted chrysalis, dewy P. longiflorum, long-rooted Oddella, etc., belongs to Basidiomycotina, Phytomycetes, Agaricaceae, White Mushrooms, and Oddella genus. Moderately high-temperature mushrooms are rare edible fungi with high medicinal and edible value. They are listed as the four famous mushrooms in my country along with matsutake, morel, and black tiger palm, and are known as the crown of mushrooms. Different from seasonal edible fungus such as shiitake mushrooms and fungus, the black-skinned fungus can be picked all year round and is very popular in the market. Gallium fruticosa has a unique taste, tender meat, rich in protein, amino acids, vitamins, and trace elements. It can be eaten raw or cooked. It is favored by people and is...

Claims

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Application Information

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IPC IPC(8): A01G18/20A01G18/00
CPCA01G18/20A01G18/00Y02P60/87
Inventor 曹天旭杜萍赵豪黄延侨黄友举
Owner YANGTZE NORMAL UNIVERSITY
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