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Method for extracting high-purity yeast beta-glucan

A glucan, high-purity technology, applied in the biological field, can solve the problems of low purity, complex extraction process, low product yield, etc., and achieve the effects of good antibacterial properties, improved antibacterial properties, and improved purity and yield.

Pending Publication Date: 2022-01-07
唐山拓普生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention proposes a method for extracting high-purity yeast β-glucan, which solves the problems of complex extraction process and low product yield and low purity in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] S1. Add 2.3 times of water to the spent beer yeast, and heat up to 45°C;

[0027] S2. Add a self-solvent of 0.03 times the water quality, heat up to 80°C and incubate for 25 minutes to autolyze the yeast. The self-solvent is a mixture of sodium bromide, ethyl acetate, and methylated vegetable oil. The mass ratio of the three is 4:7:1 ;

[0028] S3. adding the autolyzed yeast into 0.2mol / L pH 7 phosphate buffer with a mass of 10% of the autolyzed yeast to resuspend, and extracting to obtain yeast insoluble matter;

[0029] S4. Mix the yeast insoluble matter with water to make a suspension with a mass concentration of 35%, add neutral protease, mannanase, and sorbitol, perform high-pressure homogenization after enzymatic hydrolysis for 10 hours, and then centrifuge and dry to obtain yeast β-glucose Polysaccharide, high pressure homogeneous pressure 1500bar;

[0030] The mass of neutral protease is 0.1% of the mass of dry matter in the suspension, the mass of mannanase i...

Embodiment 2

[0032] S1. Add 2 times of water to the spent beer yeast, and heat up to 46°C;

[0033] S2. Add a self-solvent of 0.04 times the water quality, heat up to 81°C and incubate for 30 minutes to autolyze the yeast. The self-solvent is a mixture of sodium bromide, ethyl acetate, and methylated vegetable oil. The mass ratio of the three is 3:7:1 ;

[0034] S3. adding the autolyzed yeast into 0.2mol / L pH 7 phosphate buffer with a mass of 10% of the autolyzed yeast to resuspend, and extracting to obtain yeast insoluble matter;

[0035] S4. Mix the yeast insoluble matter with water to make a suspension with a mass concentration of 38%, add neutral protease, mannanase, and sorbitol, perform high-pressure homogenization after enzymatic hydrolysis for 9 hours, and then centrifuge and dry to obtain yeast β-glucose Polysaccharide, high pressure homogeneous pressure 1550bar;

[0036] The mass of neutral protease is 0.2% of the mass of dry matter in the suspension, the mass of mannanase is 0...

Embodiment 3

[0038] S1. Add 2.5 times of water to the spent beer yeast, and heat up to 46°C;

[0039] S2. Add a self-solvent of 0.02 times the water quality, heat up to 82°C and incubate for 23 minutes to autolyze the yeast. The self-solvent is a mixture of sodium bromide, ethyl acetate, and methylated vegetable oil. The mass ratio of the three is 3:7:1 ;

[0040] S3. adding the autolyzed yeast into 0.2mol / L pH 7 phosphate buffer with a mass of 10% of the autolyzed yeast to resuspend, and extracting to obtain yeast insoluble matter;

[0041] S4. Mix the yeast insoluble matter with water to make a suspension with a mass concentration of 34%, add neutral protease, mannanase, and sorbitol, perform high-pressure homogenization after enzymatic hydrolysis for 12 hours, and then centrifuge and dry to obtain yeast β-glucose Polysaccharide, high pressure homogeneous pressure 1450bar;

[0042] The mass of neutral protease is 0.15% of the mass of dry matter in the suspension, the mass of mannanase ...

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Abstract

The invention relates to the technical field of biology, and provides a method for extracting high-purity yeast beta-glucan. The method comprises the following steps of: S1, adding water into beer waste yeast, and heating; S2, adding a self-solvent, and heating to obtain autolytic yeast; S3, adding the autolytic yeast into a buffer solution, resuspending, and extracting to obtain yeast insoluble; and S4, mixing the yeast insoluble with water to prepare a suspension, adding neutral protease, mannase and sorbitol for enzymolysis, performing high-pressure homogenization, and performing centrifugal washing and drying to obtain the yeast beta-glucan. Through the technical scheme, the problems of complex extraction process, low product yield and low purity in the prior art are solved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for extracting high-purity yeast β-glucan. Background technique [0002] my country's beer production ranks first in the world, so my country is expected to produce about 800,000-1,200,000 tons of waste beer yeast every year. However, the recycling of waste beer yeast is limited to simple processing, and even a large amount of waste yeast is directly discharged, which cannot be rationally utilized and even causes serious pollution. [0003] The yeast cell wall accounts for about 20% of the dry weight of the cell, and 85%-90% of the yeast cell wall is polysaccharide, 10%-13% is protein, and it also contains a small amount of fat and other substances. Yeast β-glucan accounts for about 30%-50% of polysaccharides, located in the innermost layer of the cell wall, and connected with the protoplast membrane. Its main physiological function is to maintain the mechanical structure ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/06C08B37/02C12R1/865
CPCC12N1/063C08B37/0003C08B37/0024
Inventor 徐婷婷江建梅姜峰李丹丹刘立娟周雪玲刘银生陈志颖
Owner 唐山拓普生物科技有限公司
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