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BF839, bifidobacterium and facultative probiotic culture solution and preparation thereof

A bifidobacterium and probiotic technology, applied in the field of biotechnology and probiotic products, can solve the problems of increased cost, expensive and complicated products, and achieve the effects of reduced application cost, strong anti-corrosion ability and stable performance

Inactive Publication Date: 2022-01-11
优仕康生(天津)科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the invention uses broth and liver extract as the main base material, the direct formation of oral liquid preparations does not meet the taste of the public, and the preparation is complicated and the shelf life of meat products is limited. Although it can cultivate good strains, And through application practice, there are many functional effects, but because it is not suitable for directly making oral liquid with good taste, and the strain is cultivated in medium and then separated and purified to make applicable preparations, the cost will be greatly increased, making the product expensive
Therefore, this technology has not been widely used after more than 30 years

Method used

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  • BF839, bifidobacterium and facultative probiotic culture solution and preparation thereof
  • BF839, bifidobacterium and facultative probiotic culture solution and preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] A kind of batching and preparation method of BF839, bifidobacteria and facultative probiotic culture solution are the following steps:

[0050] 1. Ingredients The BF839, bifidobacterium and facultative probiotic culture solution is composed of animal protein nitrogen source, edible fungi dietary fiber nitrogen source, sugar carbon source and water according to the mass fraction ratio;

[0051] 2. Preparation Weigh 1.5-3 parts of animal protein nitrogen source, 1.5-3 parts of edible fungi dietary fiber nitrogen source, and 3-6 parts of sugar carbon source according to the mass fraction ratio, and add them together to the container and add 100-3 parts of purified water. 200 parts, boiled on high heat, decocted on low heat for 15-30 minutes, cooled to normal temperature, filtered to remove slag, and the culture medium was obtained.

[0052] The animal protein nitrogen source in the ingredients of the step 1 is the cuttlefish ink dry powder containing multiple nutrients suc...

Embodiment 2

[0056] This example is another preparation method of BF839, bifidobacteria and facultative probiotic culture solution, which is based on the ingredients in Example 1 by adding protein powder or protein peptides for nutritional enhancement, and the ratio of gram weight fraction is 0.5- 2.5%, the preparation steps are the same as in Example 1.

Embodiment 3

[0058] This embodiment is another preparation method of anaerobic and facultative probiotic culture solution, which is based on the ingredients in Example 1 or Example 2 to add probiotic cultivation promotion ingredients, the probiotic cultivation promotion ingredients and application grams The weight fraction ratio is 0.5-2.5% of casein peptone, 0.2-0.5% of oat malt, 0.2-0.5% of Nacl, and 0.2-0.5% of Nacl 2 HPO 4 0.1-0.3%, cysteine ​​0.001-0.5%, sodium thioglycolate 0.02-0.1%, yeast extract 0.1-0.8%, the preparation steps are the same as in Example 1.

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Abstract

The invention discloses a preparation method and application of a protein-philic anaerobic probiotic BF839, bifidobacterium and plant-philic nitrogen source facultative probiotic culture solution and a preparation thereof. According to the invention, cuttlefish juice and tricholoma matsutake are adopted as the main culture base materials to replace the traditional broth culture base material to prepare a culture medium solution suitable for protein-philic anaerobic probiotic fermentation and plant-philic nitrogen source facultative probiotic fermentation and can be directly prepared into a functional drink. The culture solution provided by the invention can be used as a functional drink with good stability, good taste and known primary effects of all the base materials, and can be packaged together with a live bacteria preparation after subpackaging to form a current inoculated circulating product with longer shelf life and better probiotic activity. The invention provides a preparation method of anaerobic probiotics, facultative probiotics and a probiotic oral liquid with the anaerobic probiotics and facultative probiotics. The probiotic oral liquid prepared by the method provided by the invention has the combined action of the contained probiotics and base materials, has the advantages of long shelf life, good taste and rich nutrition, and is more suitable for popularization and application.

Description

technical field [0001] The invention belongs to the field of biotechnology and probiotic products, and in particular relates to a preparation method of BF839, bifidobacterium and facultative probiotic culture solution and preparation thereof. Background technique [0002] The cultivation, isolation and utilization of human anaerobic probiotics is the most important research and development field of medicine and functional beverage biotechnology. In recent decades, my country has made remarkable achievements in this field. Among them, Professor Zhang Jijie had screened and isolated an unknown human anaerobic probiotic from the intestinal tract of well-developed infants or young animals in the 1990s, and found that it could be used in humans through experiments and clinical verification. many beneficial effects. These functions include promoting children's growth and development, preventing and treating acute and chronic enteritis, mediating flora imbalance, improving the bod...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/747A61K9/00A61P37/04A61P43/00C12N1/20C12N1/16C12Q1/04C12R1/02C12R1/01C12R1/645A61K35/745A61K35/741
CPCC12N1/20C12N1/16C12Q1/04A61K35/747A61K35/745A61K35/741A61K9/0095A61P37/04A61P43/00A61K2300/00
Inventor 刘金良
Owner 优仕康生(天津)科技发展有限公司
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